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Genome-wide analysis of the Dof transcription factors in castor bean (Ricinus communis L.)

Abstract

DNA binding with one finger (Dof) transcription factors are unique in plants, characteristically composed of a Dof domain in structure, functionally involved in mediating many processes of plant growth and development. In this study, we report a genome-wide analysis for Dof gene family in castor bean (Ricinus communis L., Euphorbiaceae) genome. In total, 21 RcDof genes were identified and their constructional characterization, classes and phylogenetic relationships were documented. The expression profiles of the 21 RcDof genes among different tissues were inspected using the high throughput RNA-Seq data. Finally, SqRT-PCR analysis was performed to test the transcriptional responses of the 21 RcDof genes to abscisic acid (ABA) and/or gibberellic acid (GA) signals, resulting in the identification of 18 RcDof genes responsive to ABA and/or GA signals. Thus, this study would provide basic information in understanding the molecular basis of the RcDof family and their potential function in regulating the growth and development of castor bean.

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Acknowledgments

We gave many thanks to Zibo Academy of Agricultural Sciences, Shandong, China for providing the seeds of castor bean var. ZB306 elite inbred line. This work was supported by the “Hundreds of Talents” program of the Chinese Academy of Sciences (AL).

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All authors declared that there is no conflict of interest.

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Correspondence to Aizhong Liu.

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Jin, Z., Chandrasekaran, U. & Liu, A. Genome-wide analysis of the Dof transcription factors in castor bean (Ricinus communis L.). Genes Genom 36, 527–537 (2014). https://doi.org/10.1007/s13258-014-0189-6

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  • DOI: https://doi.org/10.1007/s13258-014-0189-6

Keywords

  • Dof transcription factor
  • Castor bean
  • Expression profile
  • SqRT-PCR
  • Abscisic acid
  • Gibberellic acid