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CACTA transposon-derived Ti-SCARs for cultivar fingerprinting in rapeseed

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Abstract

Transposon display (TD) is a technique for analyzing polymorphic insertions and excisions of transposable elements. We used the CACTA transposon to develop cultivar-specific transposon insertion-sequence characterized amplified regions (Ti-SCARs) for rapeseed (Brassica napus). Using 16 combinations of TD primers, we detected 19 cultivar-specific fragments among six rapeseed cultivars. Of the 16 primer pairs, 12 successfully amplified targets in B. napus and six amplified novel, cultivar-specific markers. Cultivar-specific markers can be used for cultivar fingerprinting and marker-assisted selection in the rapeseed breeding program. Moreover, because Ti-SCARs are based on transposon insertions, Ti-SCAR markers may be used in reverse genetic techniques for isolating novel genes in plants.

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Correspondence to Kyong-Cheul Park or Nam-Soo Kim.

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Lee, SI., Park, KC., Ha, MW. et al. CACTA transposon-derived Ti-SCARs for cultivar fingerprinting in rapeseed. Genes Genom 34, 575–579 (2012). https://doi.org/10.1007/s13258-012-0190-x

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  • DOI: https://doi.org/10.1007/s13258-012-0190-x

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