Genes & Genomics

, Volume 34, Issue 2, pp 125–131 | Cite as

Gene expression profiles analyzed by DNA sequencing of cDNA clones constructed from porcine preadipocytes and adipocytes

  • Dong-Mok Lee
  • Prati Bajracharya
  • Eun Jeong Jang
  • Eun Ju Lee
  • Sung Hwa Chae
  • Hong-Seog Park
  • Se Won Kang
  • Yong Seok Lee
  • Ki Ho Lee
  • Jongsoo Chang
  • Chung Soo Chung
  • Inho Choi
Research Article

Abstract

As a step towards understanding the molecular mechanism of adipogenesis in pigs, preadipocytes purified from the back fat of 1 day-old female piglets were used for in vitro culture. Normalized cDNA libraries were constructed with 1.6×107 and 1.1×107 independent clones from preadipocyte and mature adipocyte mRNAs, respectively. Polymerase chain reaction (PCR) result using primers T3 and T7 (universal primer) confirmed the presence of the insert in the vector. Sequencing of 2,112 randomly selected clones from each cDNA library identified 217 clusters, 1,169 singletons, and 216 contigs in preadipocytes and 231 clusters, 1,100 singletons, and 233 contigs in mature adipocytes. Expressed sequence tag (EST) identified 24 genes with known annotation highly expressed in adipocytes and 21 in preadipocytes by at least four EST number. Among those 45 genes, when analyzed by real time RT-PCR, 76% of the gene showed significant difference between preadipocytes and mature adipocytes. Highly expressed genes in mature adipocytes were related to adipogenesis, extracellular matrix control and oncogenes, whereas cytoskeleton-related genes were down-regulated. An interesting similarity found during gene profile studies indicated a correlation between cancer and adipogenesis.

Keywords

Porcine EST Adipogenesis Gene expression cDNA library 

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Copyright information

© The Genetics Society of Korea and Springer Netherlands 2012

Authors and Affiliations

  • Dong-Mok Lee
    • 2
  • Prati Bajracharya
    • 1
  • Eun Jeong Jang
    • 1
  • Eun Ju Lee
    • 2
  • Sung Hwa Chae
    • 3
  • Hong-Seog Park
    • 4
  • Se Won Kang
    • 5
  • Yong Seok Lee
    • 5
  • Ki Ho Lee
    • 6
  • Jongsoo Chang
    • 7
  • Chung Soo Chung
    • 8
  • Inho Choi
    • 1
  1. 1.Department of BiotechnologyYeungnam UniversityGyeongsanKorea
  2. 2.School of BiotechnologyYeungnam UniversityGyeongsanKorea
  3. 3.Research Institute of GnC BioDaejeonKorea
  4. 4.Bovine Resource CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  5. 5.Department of Parasitology, College of Medicine and UHRCInje UniversityBusanKorea
  6. 6.Department of Biochemistry and Molecular BiologyEulji Medical CollegeDaejeonKorea
  7. 7.Department of Agricultural SciencesKorea National Open UniversitySeoulKorea
  8. 8.Department of Animal ScienceChungbuk National UniversityCheongjuKorea

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