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Development and characterization of microsatellite markers for genomic analysis of yarrow (Achillea millefolium L.)

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Abstract

Yarrow (Achillea millefoilum L.) is an important medicinal plant with different medicinal and ornamental uses. In this study, SSR markers were developed for the first time from the genome A. millefolium using the slightly modified Hamilton protocol. Three yarrow genomic libraries were constructed, enriched for microsatellite motifs AG, AC and ATG. A total of 30 primer pairs were developed of which 16 were polymorphic in 26 A. millefolium accessions. One accession from A. tenuifolia species was also included to assess the transferability of new developed SSR primers in other species. The average allele number of SSR markers was 8.5 per locus and ranged from 2 to 14. The observed heterozygosity (H O) varied from 0 to 0.96 with an average of 0.52 and the expected heterozygosity (H E) ranged from 0.07 to 0.49 with an average of 0.39. Among primers, Am142 showed the highest polymorphic information content (PIC) and the lowest value was obtained from Am59 primer with an average of 0.33. Three loci (AmK59, AmK344 and AmK329) deviated significantly from Hardy-Wrinberg Equlibrium (HWE) and one locus (Am344) might have null alleles. Cluster and PCoA analyses classified A. millefolium accessions according to their geographical distribution and A. tenuifolia species completely separated from A. millefoliulm genotypes.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156 83111, Iran

    Mehdi Rahimmalek & Ahmad Arzani

  2. Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156 83111, Iran

    Badraldin Ebrahim Sayed Tabatabaei

  3. Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, 84156 83111, Iran

    Mojtaba Khorrami

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  1. Mehdi Rahimmalek
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  2. Badraldin Ebrahim Sayed Tabatabaei
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  3. Ahmad Arzani
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  4. Mojtaba Khorrami
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Correspondence to Badraldin Ebrahim Sayed Tabatabaei.

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Rahimmalek, M., Sayed Tabatabaei, B.E., Arzani, A. et al. Development and characterization of microsatellite markers for genomic analysis of yarrow (Achillea millefolium L.). Genes Genom 33, 475–482 (2011). https://doi.org/10.1007/s13258-011-0045-x

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