Genes & Genomics

, Volume 32, Issue 1, pp 71–77 | Cite as

Cloning and expression pattern of a hemolin homologue from the diamondback moth, Plutella xylostella

  • Rye Jin Kim
  • Yong Hun Jo
  • Seung Han Oh
  • Cheol Hong Park
  • Mi Young Noh
  • Hyo Jeong Lee
  • Seongon Kim
  • Yong Seok Lee
  • Iksoo Kim
  • Yeon Soo Han
Research Article

Abstract

Hemolin has been known to play a key role in insect innate immunity. In an attempt to examine expression pattern of the Hemolin gene in the diamondback moth, Plutellea xylostella, the full-length cDNA of Hemolin was cloned using 5′-RACE PCR technique. The cDNA contained a 5′ untranslated region of 48 nucleotides and a 3′ untranslated region of 198 nucleotides, including a stop codon (TAA) and a poly (A) tail. It consists of 1,401 bp with an open reading frame of 1,245 bp, encoding 414 amino acids. The deduced amino acid sequence of PxHemolin has relatively low identities (35−42%) to various insect Hemolins. However, it has high three-dimensional structural similarity to Hemolin. Interestingly, analysis of spatial expression pattern of PxHemolin shows that it was highly expressed in the Malpighian tubule and the silk gland although it was also detected in fat body and gut. Furthermore, PxHemolin mRNA was highly induced 3 hr after immune-challenging with lipopolysaccharide and was gradually up-regulated after laminarin treatment. These data suggest that PxHemolin may play a role in innate immune responses although it remains to further elucidate the precise biological functions in P. xylostella.

Keywords

Plutella xylostella Hemolin Gene cloning Laminarin LPS Expression 

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Copyright information

© The Genetics Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Rye Jin Kim
    • 1
  • Yong Hun Jo
    • 1
  • Seung Han Oh
    • 1
  • Cheol Hong Park
    • 1
  • Mi Young Noh
    • 1
  • Hyo Jeong Lee
    • 1
  • Seongon Kim
    • 2
  • Yong Seok Lee
    • 3
  • Iksoo Kim
    • 1
  • Yeon Soo Han
    • 1
  1. 1.Department of Agricultural Biology, College of Agriculture and Life ScienceChonnam National UniversityGwangjuKorea
  2. 2.Jeollanam-do Agricultural Research & Extension ServicesNajuKorea
  3. 3.Department of Parasitology, College of Medicine and Frontier Inje Research for Science and TechnologyInje UniversityBusanKorea

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