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Isolation and characterization of 12 microsatellite loci from Korean water deer (Hydropotes inermis argyropus) for population structure analysis in South Korea

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Abstract

The main goals of this study were to isolate microsatellites markers of Korean water deer (Hydropotes inermis argyropus) and understand the genetic status of the species in South Korea. Twelve new microsatellite loci were isolated and characterized to establish basic population genetic parameters for 45 H. i. argyropus specimens in South Korea. There were no significant regional or genetic structure differences between the mid-eastern and southwestern populations in South Korea according to the population genetic analyses. The number of alleles per locus ranged from two to 13 with an average of 6.08. Mean expected (H E) and observed heterozygosity (H O) were 0.622 and 0.533, respectively. Microsatellite variability was also not significant between the two regions (F ST=0.012). These new markers should facilitate the future population genetics studies of Korean water deer and other closely related species.

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Authors and Affiliations

  1. Conservation Genome Resource Bank for Korean Wildlife (CGRB), Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea

    Sang-In Kim, Mi-Sook Min, Kyung-Seok Kim & Hang Lee

  2. BK21 program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea

    Yun-Sun Lee, Sung-Kyoung Choi, Junghwa An, Han-Chan Park, Mi-Sook Min & Hang Lee

  3. Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, Korea

    Kyung-Seok Kim

Authors
  1. Yun-Sun Lee
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  2. Sung-Kyoung Choi
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  3. Junghwa An
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  4. Han-Chan Park
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  5. Sang-In Kim
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  6. Mi-Sook Min
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  7. Kyung-Seok Kim
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  8. Hang Lee
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Correspondence to Kyung-Seok Kim or Hang Lee.

Additional information

Y.-S. Lee and S.-K. Choi contributed equally to this work.

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Lee, YS., Choi, SK., An, J. et al. Isolation and characterization of 12 microsatellite loci from Korean water deer (Hydropotes inermis argyropus) for population structure analysis in South Korea. Genes Genom 33, 535–540 (2011). https://doi.org/10.1007/s13258-010-0166-7

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  • DOI: https://doi.org/10.1007/s13258-010-0166-7

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