Skip to main content
Log in

Genetic variation in populations of Populus davidiana Dode based on microsatellite marker analysis

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

Populus davidiana Dode, the only aspen in Korea, is useful for restoration because of its fast growth and vigorous reproduction. This study was conducted to estimate the genetic diversity and subdivision in populations of P. davidiana in South Korea using microsatellite markers. DNA from 113 individuals from five populations was amplified using five microsatellite primers. Fifty-three alleles were detected, and the expected heterozygosity was 0.603. The populations of P. davidiana in Korea have high genetic variation despite their peripheral distribution. RST (0.213) indicated a significant level of genetic subdivision among populations compared to the differentiation among other aspen populations. The high conservation value of the populations is attributed to its ecological and commercial importance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996–2004) GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5171, Université de Montpellier II, Montpellier, France.

    Google Scholar 

  • Braatne JH, Hinckley TM, Stettler RF (1992) Influence of soil water on the physiological and morphological components of plant water balance in Populus trichocarpa, Populus deltoides and their F1 hybrids. Tree Physiol. 11: 325–339.

    PubMed  Google Scholar 

  • Bradshaw HD, Ceulemans R, Davis J, Stettler R (2000) Emerging model systems in plant biology: Poplar (Populus) as a model forest tree. J. Plant Growth Regul. 19: 306–313.

    Article  CAS  Google Scholar 

  • Cain M, Milligan B, Strand A (2000) Long-distance and seed dispersal in plant populations. Am. J. Bot. 87: 1217–1227.

    Article  PubMed  Google Scholar 

  • Cavalli-Sforza LL, Edwards AWF (1967). Phylogenetic analysis: models and estimation procedures. Am. J. Hum. Genet. 19: 233–257.

    PubMed  CAS  Google Scholar 

  • Channell R, Lomolino MV (2000) Dynamic biogeography and conservation of endangered species. Nature 403: 84–86.

    Article  PubMed  CAS  Google Scholar 

  • Chapuis M-P, Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol. Biol. Evol. 24: 621–631.

    Article  PubMed  CAS  Google Scholar 

  • Cheliak WM, Dancik BP (1982) Genic diversity of natural populations of a clone-forming tree Populus tremuloides. Can. J. Genet. Cytol. 24: 611–616.

    Google Scholar 

  • Chen X (2002) Modeling the effects of global climatic change at the ecotone of boreal larch forest and temperate forest in northeast China. Climatic Change 55: 77–97.

    Article  CAS  Google Scholar 

  • Cole CT (2005) Allelic and population variation of microsatellite loci in aspen (Populus tremuloides). New Phytol. 167: 155–164.

    Article  PubMed  CAS  Google Scholar 

  • Dayanandan S, Rajora OP, Bawa KS (1998) Isolation and characterization of microstaellites in trembling aspen (Populus tremuloides). Theor. Appl. Genet. 96: 950–956.

    Article  CAS  Google Scholar 

  • De Innocentiis S, Sola L, Cataudella S, Bentzen P (2001) Allozyme and microsatellite loci provide discordant estimates of population differentiation in the endangered dusky grouper (Epinephelus marginatus) within the Mediterranean Sea. Mol. Ecol. 10: 2163–2175.

    Article  PubMed  Google Scholar 

  • Easton EP (1997) Genetic variation and conservation of the native aspen (Populus tremula L.) resource in Scotland. Ph.D. Thesis Dissertation, The University of Edinburgh, UK.

    Google Scholar 

  • Eckert CG, Samis KE, Lougheed SC (2008) Genetic variation across species’ geographical ranges: the central-marginal hypothesis and beyond. Mol. Ecol. 17: 1170–1188.

    Article  PubMed  CAS  Google Scholar 

  • eFloras (2008) Flora of China. Missouri Botanical Garden, St. Louis, MO&Havard University Herbaria. Cambridge, MA, USA.

    Google Scholar 

  • Etterson JR, Shaw RG (2001) Constraint to adaptive evolution in response to global warming. Science 294:151–154.

    Article  PubMed  CAS  Google Scholar 

  • Fossati T, Zapelli I, Bisoffi S, Micheletti A, Vietto L, Sala F, Castiglione S (2005) Genetic relationships and clonal identity in a collection of commercially relevant poplar cultivars assessed by AFLP and SSR. Tree Genet. Genomes 1: 11–19.

    Article  Google Scholar 

  • Goodman SJ (1997) RST CALC: a collection of computer programs for calculating unbiased estimates of genetic differentiation and determining their significance for microsatellite data. Mol. Ecol. 6: 881–885.

    Article  CAS  Google Scholar 

  • Hall D, Luquez V, Garcia VM, Onge KRS, Jasson S, Ingvarsson PK (2007) Adaptive population differentiation in phenology across a latitudinal gradient in European aspen (Populus tremula L.): A comparison of neutral markers, candidate genes and phenotypic traits. Evolution 61: 2849–2860.

    Article  PubMed  Google Scholar 

  • Hamrick JL, Godt MJW, Sherman-Broyles SL (1992) Factors influencing levels of genetic diversity in woody plant species. New Forest. 6: 95–124.

    Article  Google Scholar 

  • Hong KN (1997) Molecular phylogeny of section Leuce and genetic variation of natural pouplations of Populus davidiana in Korea based on RAPD marker analysis. Ph.D. Thesis Dissertation, Seoul National University, Korea.

    Google Scholar 

  • Hyun JO, Rajora OP, Zsufffa L (1987) Genetic variation in trembling aspne in Ontario based on isozyme studies. Can. J. Forest Res. 17: 1134–1138.

    Article  Google Scholar 

  • Imbert E, Lefèvre F (2003) Dispersal and gene flow of Populus nigra (Salicaceae) along a dynamic river system. J. Ecol. 91: 447–456.

    Article  Google Scholar 

  • Ingvarsson PK (2005) Nucleotide polymorphism and linkage disequilibrium within and among natural populations of European aspen (Populus tremula L., Salicaceae). Genetics 169: 945–953.

    Article  PubMed  CAS  Google Scholar 

  • Jelinski DE, Cheliak WM (1992) Genetic diversity and spatial subdivision of Populus tremuloides (Salicaceae) in a heterogeneous landscape. Am. J. Bot. 79: 728–736.

    Article  Google Scholar 

  • Kalinowski ST (2004) Counting alleles with rarefaction: Private alleles and hierarchical sampling designs. Conserv. Genet. 5: 539–543.

    Article  CAS  Google Scholar 

  • Kamiya K, Moritsuka E, Yoshida T, Yahara T, Tachida H (2008) High population differentiation and unusual haplotype structure in a shade-intolerant pioneer tree species, Zanthoxylum ailanthoides (Rutaceae) revealed by analysis of DNA polymorphism at four nuclear loci. Mol. Ecol. 17: 2329–2338.

    Article  PubMed  CAS  Google Scholar 

  • Komarova TA (1992) Post-fire forest successions in the southern part of Sikhote-Alin Region. Dalnauka, Vladivostok, Russia.

    Google Scholar 

  • Kramer P, Kozlowski T (1979) Physiology of woody plants. Academic press, New York, NY, USA.

    Google Scholar 

  • Latva-Karjanmaa T (2006) Reproduction and population structure in the European aspen. Doctoral Thesis Dissertation, University of Helsinki, Finland.

    Google Scholar 

  • Lenormand T (2002) Gene flow and the limits to natural selection. Trends Ecol. Evol. 17: 183–189.

    Article  Google Scholar 

  • Lhuillier E, Butaud JF, Bouvet J-M (2006) Extensive clonality and strong differentiation in the insular pacific tree Santalum insulare: Implications for its conservation. Ann. Bot. (Lond) 98: 1061–1072.

    Article  CAS  Google Scholar 

  • Li K, Zhang F, Bao G, Shi J (1999) Research advances in genetics and breeding of Populus davidiana Dode in China. J. Forest. Res. 10: 25–30.

    Article  CAS  Google Scholar 

  • Li Q, Kijima A (2002) Identification of novel microsatellite loci in the Pacific oyster (Crassostrea gigas) by magnetic bead hybridization selection. Tohoku J. Agric. Res. 53: 25–32.

    CAS  Google Scholar 

  • Liu K, Muse SV (2005) PowerMarker: Integrated analysis environment for genetic marker data. Bioinformatics 21: 2128–2129.

    Article  PubMed  CAS  Google Scholar 

  • Lowe A, Harris S, Ashton P (2004) Ecological Genetics: Design, Analysis, and Application. Blakwell Publishing, Malden, MA, USA.

    Google Scholar 

  • MacGowan I (1993) The entomological value of aspen in the Scottish highlands. Malloch Society Research Report No. 1. Malloch Society, UK.

    Google Scholar 

  • Mayr E (1970) Populations, species, and evolution. Harvard University Press, Cambridge, MA, USA.

    Google Scholar 

  • Mock KE, Rowe CA, Hooten MB, Dewoody J, Hipkins VD (2008) Clonal dynamics in western North American aspen (Populus tremuloides). Mol. Ecol. 17: 4827–4844.

    Article  PubMed  CAS  Google Scholar 

  • Namroud M-C, Park A, Tremblay F, Bergeron Y (2005) Clonal and spatial genetic structures of aspen (Populus tremuloides Michx.). Mol. Ecol. 14: 2969–2980.

    Article  PubMed  CAS  Google Scholar 

  • Noh ER, Lee SK, Koo YB, Chung KH (1988) A mass propagation method of aspen (Populus davidiana Dode) using tissue culture and juvenile cutting techniques. Rep. Inst. For. Gen. Korea. 24: 20–27.

    Google Scholar 

  • Noh ER, Lee SK, Koo YB, Shim SY, Park HR (1989) Growth patterns and variation of some economic characteristics in selected Korean aspen (Populus davidiana Dode). Rep. Inst. For. Gen. Korea. 25: 11–29.

    Google Scholar 

  • Noh ER, Lee SK, Koo YB (1990) Determination of early selection age using age-to-age correlation in Populus davidiana Dode. Rep. Inst. For. Gen. Korea. 26: 10–21.

    Google Scholar 

  • Parmesan C (2006) Ecological and evolutionary responses to recent climate change. Annu. Rev. Ecol. Evol. Syst. 3: 637–669.

    Article  Google Scholar 

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Resour. 6: 288–295.

    Google Scholar 

  • Rahman MH, Rajora OP (2002) Microsatellite DNA fingerprinting, differentiation, and genetic relationships of clones, cultivars, and varieties of six poplar species from three sections of the genus Populus. Genome 45: 1083–1094.

    Article  PubMed  CAS  Google Scholar 

  • Rousset F (2008) GENEPOP’ 007: a complete reimplementation of the GENEPOP software for Windows and Linux. Mol. Ecol. Resour. 8: 103–106.

    Article  Google Scholar 

  • Smith S, Hughes J, Wardell-Johnson G (2003) High population differentiation and extensive clonality in a rare mallee eucalypt: Eucalyptus curtisii. Conserv. Genet. 4: 289–300.

    Article  CAS  Google Scholar 

  • Smulders MJM, Shoot JVD, Arens P, Vosman B (2001) Trinucleotide repeat microsatellite markers for black poplar (Populus nigra L.). Mol. Ecol. 1: 188–190.

    CAS  Google Scholar 

  • Suvanto LI, Latva-Karjanmaa TB (2005) Clone identification and clonal structure of the European aspen. Mol. Ecol. 14: 2851–2860.

    Article  PubMed  CAS  Google Scholar 

  • Szpeich ZA, Jakobsson M, Rosenberg NA (2008) ADZE: a rarefaction approach for counting alleles private to combinations of populations. Bioinformatics 24: 2498–2504.

    Article  Google Scholar 

  • Takezaki N, Nei M (1996) Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics 144: 389–399.

    PubMed  CAS  Google Scholar 

  • Thrall PH, Burdon JJ, Murray BR (2000) The metapopulation paradigm: a fragmented view of conservation biology. In. Young, A. G., Clarke, G. M. (Eds.), Genetics, demography and viability of fragmented populations, Conservation Biology. Cambridge University Press, Cambridge, UK.

    Google Scholar 

  • Tuskan GA, Gunter LE, Yang ZK, Yin T, Sewell MM, DiFazio SP (2004) Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa. Can. J. Forest. Res. 34: 85–93.

    Article  CAS  Google Scholar 

  • Vucetich JA, Waite TA (2003) Spatial patterns of demography and genetic processes across the species’ range: Null hypotheses for landscape conservation genetics. Conser. Genet. 4: 639–645.

    Article  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38: 1358–1370.

    Article  Google Scholar 

  • Worrell R (1995) European aspen (Populus tremula L.): a review with particular reference to Scotland II. Values, silviculture and utilization. Forestry 68: 231–244.

    Article  Google Scholar 

  • Wyman J, Bruneau A, Tremblay M-F (2003) Microsatellite analysis of genetic diversity in four populations of Populus tremuloides in Quebec. Can. J. Botany 81: 360–367.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yong Yul Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, K.M., Kim, Y.Y. & Hyun, J.O. Genetic variation in populations of Populus davidiana Dode based on microsatellite marker analysis. Genes Genom 33, 163–171 (2011). https://doi.org/10.1007/s13258-010-0148-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-010-0148-9

Keywords

Navigation