Fabrication and Testing of Electrospun Polyurethane Blended with Chitosan Nanoparticles for Vascular Graft Applications
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In this study, a small vascular graft based on polyurethane (PU) blended with chitosan (Ch) nanoparticles was fabricated using electrospinning technique. Initially, the chitosan nanoparticles were synthesized using ionic gelation method. UV–Vis spectrophotometer confirmed the presence of synthesized Ch nanoparticles by exhibiting absorption peak at 288 nm and the Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the existence of the chitosan. Further, the synthesized Ch nanoparticles showed size diameter in the range of 134 ± 58 nm as measured using ImageJ. In the electrospun PU/chitosan graft, the fiber diameter and pore size diameter was found to be reduced compared to the pure PU owing to incorporation of chitosan into PU matrix. The FTIR spectrum revealed the presence of chitosan in the prepared nanocomposite membrane by the formation of the hydrogen bond and peak shift of CH and NH stretching. Moreover, the contact angle measurements revealed that the prepared graft showed decreased contact angle indicating hydrophilic nature compared to the pristine PU. The cytocompatibility studies revealed the non-toxic behavior of the fabricated graft. Hence, the prepared graft exhibiting significant physiochemical and non-toxic properties may be a plausible candidate for cardiovascular graft applications.
KeywordsPolyurethane/chitosan membrane Ionic gelation method Electrospinning Physiochemical characterization Vascular graft
This work was supported by the Ministry of Higher Education Malaysia with the Grant Number Q.J130000.2545.14H59.
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Alhosseini, S. N., F. Moztarzadeh, M. Mozafari, S. Asgari, M. Dodel, A. Samadikuchaksaraei, S. Kargozar, and N. Jalali. Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering. Int. J. Nanomed. 7:25, 2012.Google Scholar
- 2.Anicuta, S. G., L. Dobre, M. Stroescu, and I. Jipa. Fourier transform infrared (FTIR) spectroscopy for characterization of antimicrobial films containing chitosan. Analele Universită Ńii din Oradea Fascicula: Ecotoxicologie, Zootehnie şi Tehnologii de Industrie Alimentară 1234–1240, 2010.Google Scholar
- 12.Jaganathan, S. K., M. P. Mani, M. Ayyar, N. P. Krishnasamy, and G. Nageswaran. Blood compatibility and physicochemical assessment of novel nanocomposite comprising polyurethane and dietary carotino oil for cardiac tissue engineering applications. J. Appl. Polym. Sci. 2017. https://doi.org/10.1002/app.45691.Google Scholar
- 21.Mano, J. F., G. A. Silva, H. S. Azevedo, P. B. Malafaya, R. A. Sousa, S. S. Silva, L. F. Boesel, J. M. Oliveira, T. C. Santos, A. P. Marques, and N. M. Neves. Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J. R. Soc. Interface 4(17):999–1030, 2007.CrossRefGoogle Scholar
- 28.Subramaniam, A., and S. Sethuraman. Biomedical applications of nondegradable polymers. Nat. Synth. Biomed. Polym. 2014. https://doi.org/10.1016/B978-0-12-396983-5.00019-3.Google Scholar
- 29.Syazana, N., and I. Sukmana. Electrospun-based fibrous scaffold for cardiovascular engineering applications: a review. J. Eng. Appl. Sci. 11(7):4778–4781, 2006.Google Scholar
- 33.Wang, H., Y. Feng, B. An, W. Zhang, M. Sun, Z. Fang, W. Yuan, and M. Khan. Fabrication of PU/PEGMA crosslinked hybrid scaffolds by in situ UV photopolymerization favoring human endothelial cells growth for vascular tissue engineering. J. Mater. Sci. 23(6):1499–1510, 2012.Google Scholar