Cardiovascular Engineering and Technology

, Volume 5, Issue 1, pp 119–131 | Cite as

Cardiac Fibroblast-Derived 3D Extracellular Matrix Seeded with Mesenchymal Stem Cells as a Novel Device to Transfer Cells to the Ischemic Myocardium

  • Eric G. Schmuck
  • Jacob D. Mulligan
  • Rebecca L. Ertel
  • Nicholas A. Kouris
  • Brenda M. Ogle
  • Amish N. Raval
  • Kurt W. Saupe
Article

Abstract

Demonstrate a novel manufacturing method to generate extracellular matrix scaffolds from cardiac fibroblasts (CF-ECM) as a therapeutic mesenchymal stem cell-transfer device. Rat CF were cultured at high-density (~1.6 × 105/cm2) for 10–14 days. Cell sheets were removed from the culture dish by incubation with EDTA and decellularized with water and peracetic acid. CF-ECM was characterized by mass spectrometry, immunofluorescence and scanning electron microscopy. CF-ECM seeded with human embryonic stem cell derived mesenchymal stromal cells (hEMSCs) were transferred into a mouse myocardial infarction model. 48 h later, mouse hearts were excised and examined for CF-ECM scaffold retention and cell transfer. CF-ECM scaffolds are composed of fibronectin (82%), collagens type I (13%), type III (3.4%), type V (0.2%), type II (0.1%) elastin (1.3%) and 18 non-structural bioactive molecules. Scaffolds remained intact on the mouse heart for 48 h without the use of sutures or glue. Identified hEMSCs were distributed from the epicardium to the endocardium. High density cardiac fibroblast culture can be used to generate CF-ECM scaffolds. CF-ECM scaffolds seeded with hEMSCs can be maintained on the heart without suture or glue. hEMSC are successfully delivered throughout the myocardium.

Keywords

Cardiac fibroblast Extracellular matrix Stem cell Cardiac Regeneration Heart failure Myocardial infarction 

Supplementary material

Supplementary material 1 (MPEG 11638 kb)

13239_2013_167_MOESM2_ESM.mpeg (11.4 mb)
Supplementary material 2 (MPEG 1174 kb)

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Copyright information

© Biomedical Engineering Society 2013

Authors and Affiliations

  • Eric G. Schmuck
    • 1
    • 3
  • Jacob D. Mulligan
    • 1
  • Rebecca L. Ertel
    • 1
  • Nicholas A. Kouris
    • 2
  • Brenda M. Ogle
    • 2
  • Amish N. Raval
    • 1
    • 2
  • Kurt W. Saupe
    • 1
  1. 1.Department of MedicineUniversity of Wisconsin at MadisonMadisonUSA
  2. 2.Department of Biomedical EngineeringUniversity of Wisconsin at MadisonMadisonUSA
  3. 3.H6/385 Clinical Science CenterMadisonUSA

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