Cryo-EM structures of the mammalian endo-lysosomal TRPML1 channel elucidate the combined regulation mechanism
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TRPML1 channel is a non-selective group-2 transient receptor potential (TRP) channel with Ca2+ permeability. Located mainly in late endosome and lysosome of all mammalian cell types, TRPML1 is indispensable in the processes of endocytosis, membrane trafficking, and lysosome biogenesis. Mutations of TRPML1 cause a severe lysosomal storage disorder called mucolipidosis type IV (MLIV). In the present study, we determined the cryo-electron microscopy (cryo-EM) structures of Mus musculus TRPML1 (mTRPML1) in lipid nanodiscs and Amphipols. Two distinct states of mTRPML1 in Amphipols are added to the closed state, on which could represent two different confirmations upon activation and regulation. The polycystin-mucolipin domain (PMD) may sense the luminal/extracellular stimuli and undergo a “move upward” motion during endocytosis, thus triggering the overall conformational change in TRPML1. Based on the structural comparisons, we propose TRPML1 is regulated by pH, Ca2+, and phosphoinositides in a combined manner so as to accommodate the dynamic endocytosis process.
KeywordsmTRPML1 mucolipidosis type IV structual comparisons combined regulation mechanism
Mucolipidosis type IV (MLIV), first identified in 1974 (Berman et al., 1974; Bundey et al., 1974; Venkatachalam et al., 2015), is a severe autosomal recessive lysosomal storage disorder disease, with around one occurrence in every 40,000 people and 70 percent of the affected individuals are Ashkenazi Jewish (Bach, 2005; Bargal et al., 2001; Puertollano and Kiselyov, 2009). Classified as a mucolipidosis, MLIV exhibits a simultaneous lysosomal accumulation of granulated water-soluble materials and membranous lipid substances in the tissue and organelles of these patients (Bach, 2005; Zeevi et al., 2007). Children afflicted with MLIV often show psychomotor retardation, iron deficiency, retinal degeneration, and motor deficits in the early stage of their life and those who survive to adulthood usually have a shortened lifespan (Sun et al., 2000; Weitz and Kohn, 1988). Mutations in MCOLN1, a gene that encodes TRPML1 (mucolipin-1), have been found to cause MLIV (Bargal et al., 2000; Sun et al., 2000). TRPML1 is characterized as a non-selective, cation-permeable channel (Venkatachalam et al., 2015), which might mediate the release of Ca2+ from lysosomal or late endosomal lumen into the cytosol (Cheng et al., 2010; Cheng et al., 2015), thus regulating membrane trafficking, lysosomal biogenesis, and signal transduction (Colletti and Kiselyov, 2011; Samie et al., 2013; Wang et al., 2014).
TRPML1 belongs to the family of transient receptor potential mucolipin channels (TRPML channels) (Benemei et al., 2015; Clapham, 2003; Clapham et al., 2001; Flockerzi, 2007; Gees et al., 2012) and this family is the only cation channels known so far to be localized and function in the digestive tract (Colletti and Kiselyov, 2011). TRP channels consist of six sub-families, TRPA, TRPC, TRPM, TRPP, TRPV, and TRPML, classified by sequence homology (Han and Wang, 2008; Minke, 2006; Pedersen et al., 2005; Ramsey et al., 2006). Meanwhile, according to topology similarities, they are divided into two distinct groups (group 1 and group 2) (Venkatachalam and Montell, 2007). Group 2 contains two members, TRPP and TRPML (Qian and Noben-Trauth, 2005), as they have a large extracellular loop between transmembrane segments 1 and 2, which differs significantly from group 1 TRP subfamily (Grieben et al., 2017; Shen et al., 2016; Wilkes et al., 2017). Structural studies empowered by the great improvement of single particle cryo-EM method have revealed significant differences between the two groups. Recently, three individual research groups have reported the structures of TRPP2 determined by cryo-EM with three different states named TRPP2CL (closed) (Grieben et al., 2017; Shen et al., 2016), TRPP2MI (multi-ion), and TRPP2SI (single-ion) (Wilkes et al., 2017) and these distinct structures were presented to elucidate the possible gating and regulatory mechanisms of group 2 channels.
Unlike other plasma ion channels, TRPML1 predominately resides on late endosome and lysosome membrane during endocytosis, and also temporarily resides on cell surface plasma during exocytosis (Cheng et al., 2010). The di-leucine motifs in both N- and C-terminus mediate its localization to late endosome and lysosome (Venkatachalam et al., 2015; Vergarajauregui and Puertollano, 2006). Two models of regulatory mechanism were proposed in terms of how to ensure proper level of the channel activity. One model is the subcellular-compartment-dependent regulation by phosphoinositide. In this model, different phosphoinositide isoform may regulate the channel activity through direct binding to the N-terminus lipid-interacting domain (spanning amino acid residues 42–62). PI(3,5)P2, a specific late endosome/lysosome phosphoinositide, may activate TRPML1 channel during membrane trafficking and lysosome biogenesis (Dong et al., 2010a; Waller-Evans and Lloyd-Evans, 2015). On the contrary, a plasma membrane specific phosphoinositide, PI(4,5)P2 may function to inhibit TRPML1, thus shutting down excess Ca2+ influx from extracellular matrix to cytosol, which in turn could lead to cell death (Zhang et al., 2012). The other model of regulatory mechanism is the pH and Ca2+ dual regulation of the PMD. This dual regulation is achieved through different pH and Ca2+ levels in different organelles. Late endosome have 0.5 mmol/L Ca2+, which is about 1000 fold to the concentration of Ca2+ in cytosol, and an acidic pH of 5.5–6.0. Meanwhile, lysosomes have a more acidic pH of 4.5–5.0 to supply acid environment for native hydrolases (Appelqvist et al., 2013) and 0.5–0.6 mmol/L Ca2+. The extracellular side of the plasma membrane has a neutral pH of 7.2 to 7.4 and an obviously higher concentration of Ca2+ (1.8–2.0 mmol/L) (Dong et al., 2010b). High concentration of Ca2+ was shown to inhibit the current of TRPML1 (V432P) channel in whole-cell patch-clamp recording (Li et al., 2017; Xu et al., 2007). In late endosome and lysosome, luminal Ca2+ inhibition of TRPML1 attenuates following the pH decrease from 7.4 to 5.5 and 4.5, respectively. Minghui Li et al. solved the structures of this PMD at different pH conditions, which provides structural basis of TRPML1’s regulation by proton and Ca2+ and suggested that the protonation of 12 aspartates in the luminal pore at different pH conditions is responsible for the attenuation (Li et al., 2017).
However, elucidating how TRPML1 responds to different pH, Ca2+, and phosphatidylinositol during endocytosis and exocytosis has been hampered by a lack of overall structure information (Waller-Evans and Lloyd-Evans, 2015; Zhang et al., 2012). Here, using single-particle electron cryo-microscopy (cryo-EM) method, we determined the structure of mTRPML1 in lipid nanodiscs at a resolution of 5.4 Å. Moreover, in order to mock the subcellular locations of TRPML1 and interpret compartment regulation mechanism, we solved the structures of mTRPML1 in Amphipols at resolutions of 5.8 (state 1), 7.4 (state 2), and 7.7 Å (state 3), respectively. Structure comparisons between these three conformations of TRPML1 in Amphipols have revealed the potential channel gating mechanism and sheds light on the regulation of compartment specificity of TRPML1 channel.
Structure determination of mTRPML1
As TRPML1 mainly functions in the dynamically endocytosis/exocytosis process, the structure and function of the channel might be different in order to accommodate to various environment. With the aim of seizing possible different states of TRPML1, we reconstituted mTRPML1 in Amphipols after purification with n-dodecyl-β-D-maltoside (DDM). Interestingly, after rounds of 3D classification with sufficient tests, three conformational states of mTRPML1 were identified at resolutions of 5.8 (state 1), 7.4 (state 2), and 7.7 Å (state 3) (Fig. S4), respectively. These different states led us to speculate that those might result from different subcellular locations of mTRPML1 during endocytosis/exocytosis pathway and could shed light on the regulation mechanism of TRPML1.
Overall structure of mTRPML1
Structural comparison between mTRPML1 in lipid nanodiscs and in Amphipols (state 1) (Figs. 1B, 1D, S4, and S5) reveals that these two structures exhibit indistinguishable channel features due to lower resolution, except that the mTRPML1 in Amphipols lacks the densities for N-terminus, C-terminus and S2–S3 loop. The emerging N-terminus (36–58) contains the phosphatidylinositol binding sites (Zhang et al., 2012). It appears that soy extract polar lipid helps to stabilize the channel integrity and helps us obtain the higher resolution structure. Four mTRPML1 subunits form a tetrameric architecture with each subunit consisting of six transmembrane helices and a re-entrant pore loop intervening transmembrane helix S5 and S6 (Fig. 1A and 1C). The voltage-sensing domain (VSD) consists of the S1 to S4 helices and is similar to other voltage-gated ion channels (VGICs) (Long et al., 2005) and TRP channels (Cao et al., 2013; Huynh et al., 2016; Paulsen et al., 2015) (Fig. S6).
Apart from this canonical and conserved transmembrane region, mTRPML1 contains a large luminal/extracellular PMD between the transmembrane helix S1 and S2 (Fig. 1A and 1C). The PMD resides upon the lipid bilayer of the plasma membrane, endosome membrane and lysosome membrane. This tightly packed tetrameric ring structure consists of two long α-helices (α1 and α2), two short α-helices (α3 and α4), eight β-strands and three loops (Fig. 1A and 1C) (Li et al., 2017). Accounting for 1/3 of the ion channel mass, this PMD only exists in TRPML and TRPP families, and it contributes not only to the channel assembly by homotypic interaction but also to the allosterically regulation and channel gating. Furthermore, the linker between S2 and S3 protrudes toward intracellular, which is different from TRPP2 (Grieben et al., 2017; Shen et al., 2016; Wilkes et al., 2017). Interestingly, this long extension lays close to the N-terminus phosphatidylinositol binding sites (36–58) and C-terminus helix (536–543). High resolution structure will elaborate the possible function of this intracellular interaction.
Glycosylation of TRPML1 is an indispensable process in channel function (Hofherr et al., 2014; Kiselyov et al., 2005; Zeevi et al., 2007). mTRPML1 has four proposed N-glycosylation sites (Asn159, Asn179, Asn220, Asn230) and the modification occurs at the consensus NX(T/S) motif (X stands for any amino acid) (Wang et al., 2014). The density shows that two asparagine residues (Asn159, Asn230) are obviously N-glycosylated (Fig. S7). Asn179 and Asn220 are adjacent to the disordered region and are not resolved in this structure. It remains intriguing whether N-glycosylation will trigger the conformational change or whether different states will adopt different patterns of N-glycosylation.
Continuous connection between S1 and PMD
Ion translocation pathway in mTRPML1
Three different states of mTRPML1 in Amphipols
To gain further insight into the differences between these three states, we transform the constructed model structures into density maps and slice a vertical section across the transmembrane regions (Fig. 4B). Intuitively, compared with state 1, state 2 and 3 harbor a longer structure in the vertical direction. An amphipathic tunnel between pore filter region and PMD enlarges the cave pocket and contributes to the vertical extension (Fig. 4C). This tunnel enlargement was due to the movement of the outer PMD and accompanied by a twist between VSD region and pore filter region. The main difference between state 2 and state 3 lies in the transmembrane filter region with a slight twist occurring in the helix S5 and S6 (Fig. 4C).
Similar to other TRP channel structures, the central ion permeation pathway of the TRPML1 exhibits two major canonical constrictions gates. We have discussed the difference in lower gate between state 1 mTRPML1 channel and other TRPP2 channels above. Measurement of the upper pore helix gate in three mTRPML1 states shows that state 1 seems to adopt a narrow upper gate and state 2 has the widest upper gate. Continuing down the pore, when measured the distances between Cα-Cα in the same residue of the lower gate, state 1 still exhibit a narrow gate whereas state 3 has the widest lower gate. Taken together, state 1 seems to be the narrowest ion permeation state and represents the close state. State 3 has a wider lower filter gate, while state 2 has a wider upper pore restriction (Fig. 5D). This comparison is consistent with the three states of TRPP2, yet the more opened state TRPP2-SI has a wider lower gate than TRPP2-MI but exhibits a narrow pore helix upper gate to TRPP2-MI (Grieben et al., 2017; Shen et al., 2016; Wilkes et al., 2017). Superposition of state 1 and 3 in the transmembrane helix region resulted in a conformational twist and shift in both VSD and filter region, and this swivel is accompanied with conformational change in the outer PMD (Fig. 5C). In addition, comparison of the S1 and α1 helix region between all three states is consistent with our hypothesis that the continuous helix twist could mediate conformational change transduction through the outer PMD to the transmembrane region (Fig. 5C).
Structure interpretation of mucolipidosis type IV pathogenesis
MLIV is a severe neurodegenerative lysosome storage disorder disease caused by mutations in MCOLN1 gene, which locates on chromosome 19p13.2-p13.3 (Zeevi et al., 2007). There are more than 20 MCOLN1 mutations identified in MLIV patients. Among them, two major founded MCOLN1 mutations in Ashkenazi Jewish patients account for 95% of disease-associated alleles (Bargal et al., 2001; Venkatachalam et al., 2015). The most common one is a splice site mutation g.5534A→G that comprises 72% patients in Ashkenazim Jewish population resulting in aberrant splicing and truncated unstable mRNA species (Wakabayashi et al., 2011). Another common mutation comprising 23% patients is caused by a ∼6 kb deletion spanning the first six exons as well as a 12 bp deletion of exon 7 (Zeevi et al., 2007). Other mutations include nonsense mutations, missense mutations and in-frame deletion (Everett, 2011).
Although biological studies of TRPML1 have been extensively carried out in the past (Ahuja et al., 2016; Cheng et al., 2010; Colletti and Kiselyov, 2011; Waller-Evans and Lloyd-Evans, 2015; Wang et al., 2014), the molecular mechanism of how the TRPML1 channel gating remains elusive, largely due to the lack of detailed structural information. In the present study, we embarked on solving the structure of mTRPML1 and obtained the different conformational states in nanodiscs and Amphipols. In vitro reconstruction of mTRPML1 in lipid nanodiscs could represent the native and comprehensive cellular environment to a large extent. Soy polar extract is a natural product generated from soybean and this hockey-puck-like structure mimic the native lipid environment (Gao et al., 2016; Ritchie et al., 2009). Phosphatidylinositol, especially the PI(4,5)P2 from the soybean polar could inhibit the channel activity and helps stabilize the structure through the N-terminus binding site (Zhang et al., 2012). In addition, we added 5 mmol/L CaCl2 during protein purification and nanodiscs reconstruction. Therefore, we speculated this lipid chimeric structure represents a channel-closed state. Whereas in the reconstruction of mTRPML1 in Amphipols A8–35, we did not add extra CaCl2 and the purification buffer was maintained at pH 7.4, which could not provide a sustaining Ca2+ inhibition on PMD. High-level overexpression of TRPML1 will also drive TRPML1 from endo-lysosome to plasma membrane (Zhang et al., 2012). Hence, in the initial detergent (DDM) extraction step, mTRPML1 in different organelles with different conformations were obtained. It is possible that the three different structural states represent different “energy” states, or intermediate states, which can be converted to different functional states by additional regulatory factors, such as extracellular pH, phosphatidylinositol and Ca2+. We speculate therefore that State 1 may represent the channel-closed state in the environment of pH 7.4 when TRPML1 resides on the plasma membrane and State 2, 3 adopt relatively opened conformation, which exhibited as transition states involved in the endocytosis trafficking process.
The heterogeneity of TRPML1 combines the cellular organelle compartment with different regulatory factors, including pH, Ca2+ and phosphoinositide. Our structural analyses indicate that the TRMPL1 protein is regulated in a combined manner with these regulatory factors. High-resolution structure of TRPML1 will further replenish the regulation mechanism during endocytosis and provide a chance to better understand their roles in mucolipidosis pathogenesis.
Materials and Methods
HEK293F cells were cultured in SMM 293-TI medium (Sino Biological Inc.) supplemented with 1× penicillin/streptomycin (Solarbio) at 37°C with 8% CO2.
For protein expression, mTRPML1 was cloned in frame with a C-terminus strep-tag II (WSHPQFEK) into the plasmid pcDNA3.1 (-). Transient transfection was performed to heterogeneously express the target protein. In brief, for one liter culture of HEK293F cells, 1 mg plasmids was pre-incubated with 4 mg 25-kDa linear polyethylenimine (PEI) (Polysciences) in 50 mL fresh medium for 30 min prior to adding the mixture to cells. The transfected cells were cultured for 48 h before harvesting.
Protein purification and reconstructed in Amphipols A8–35
For each batch of protein purification, two liters of transfected HEK293F cells were harvested by centrifugation at 3,000 ×g. Cell pellets were resuspended in lysis buffer containing 20 mmol/L Hepes, pH 7.4, and 150 mmol/L NaCl, 1 μg/mL leupeptin, 1.5 μg/mL pepstatin, 0.84 μg/mL aprotinin, 0.3 mmol/L PMSF and lysed by sonication for 5 min. Cell membrane was pelleted after a 100,000 ×g ultracentrifugation for 1 h. Membrane was resuspended in buffer containing 20 mmol/L Hepes, pH 7.4, 150 mmol/L NaCl, 2 mmol/L DTT, and 1% (w/v) DDM (Anatrace) for 2 h with gentle rotation at 4°C. After ultra-centrifugation at 100,000 ×g for 20 min, the supernatant was incubated with Strep-Tactin Sepharose (IBA) for 1 h with gentle rotation at 4°C. The resin was washed extensively with wash buffer containing 20 mmol/L Hepes, pH 7.4, 150 mmol/L NaCl, 2 mmol/L DTT, 0.02% (w/v) DDM. The target mTRPML1 protein was eluted with wash buffer plus 5 mmol/L D-Desthiobiotin (IBA). The eluted protein was then mixed with Amphipols A8–35 at 1:3 (w/w) with gentle agitation for another 4 h. Detergent was removed with Bio-Beads SM-2 at 4°C overnight (15 mg per 1 mL channel/detergent/amphipols mixture). The reconstitution mixture was cleared by centrifugation before applied to size-exclusion chromatography (Superpose-6 10/300 GL, GE Healthcare) in buffer containing 20 mmol/L Hepes, pH 7.4, 150 mmol/L NaCl, 2 mmol/L DTT). The peak corresponding to tetrameric mTRPML1 channel reconstituted in Amphipols A8–35 was collected for further cryo-microscopy analysis.
Protein purification and reconstructed in lipid nanodiscs
A similar protocol was used to purify mTRPML1 in nanodiscs during membrane extraction and affinity chromatography, except that 5 mmol/L CaCl2 was added in all buffers throughout purification since calcium was reported to stabilize TRPML1 channels (Li et al., 2017). Membrane scaffold protein MSP2N2 was expressed and purified from E. coli (Civjan et al., 2003; Ritchie et al., 2009) and lipid nanodiscs was reconstructed as described (Gao et al., 2016). In brief, soybean polar lipid extract (Avanti) dissolved in chloroform was dried under an argon stream and residual chloroform was further removed by vacuum desiccation (3 h). 10 mmol/L lipids stock was prepared by resuspending dried lipids in a buffer containing 20 mmol/L HEPES (pH 7.4), 150 mmol/L NaCl, 14 mmol/L DDM via bath sonication. Purified mTRPML1 protein (0.7 mg/mL) solubilized in 0.5 mmol/L DDM was mixed with MSP2N2 (3 mg/mL) and the soybean lipid stock at a molar ratio of 1:2:200, followed by incubate on ice for 30 min. Bio-beads SM2 (30mg per 1mL reconstitution mixture; Bio-Rad) were added to remove detergents and initiate lipid nanodiscs reconstitution at 4°C for 1 h with gentle rotation. Bio-beads were then removed and the reconstitution mixture was cleared by centrifugation before subsequent separation on a Superose 6 column (GE Heath Care) in buffer (20 mmol/L HEPES, 150 mmol/L NaCl, pH 7.4, 5 mmol/L CaCl2). The peak corresponding to mTRPML1 channel reconstituted in lipid nanodiscs was collected for electron cryo-microscopy analyses.
The cryo-EM grids were prepared using Vitrobot Mark IV (FEI) at 4°C and 100% humidity. For samples of both mTRPML1 amphipols and nanodiscs, 4 μL aliquots of samples at concentrations of 0.5–0.7 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). After a waiting time of 3 s, the grids were blotted for 1–2 s and plunged into liquid ethane for quick freezing.
The grids were screened on a Tecnai Arctica microscope (FEI) operated at 200 kV using a Falcon II direct electron detector (FEI). The qualified grids were transferred into a Titan Krios microscope (FEI) operated at 300 kV for data acquisition. Images were recorded using a K2 submit direct electron detector (Gatan) in a counting mode at a nominal magnification of 22,500×, corresponding to a calibrated pixel size of 1.32 Å at object scale, and with defocus ranging from 1.7–2.6 μm. Date acquisition were performed semi-automatically using UCSF-Image4 (Li et al., 2015) in a movie mode, with a dose rate of 8.2 counts (10.9 electrons) per pixel size per second for a total exposure time of 8 s. Each micrographs were stored as a movie stack with 32 frames.
2,565 and 3,944 micrographs (movie stacks) are collected for mTRPML1 amphipols and mTRPML1 nanodiscs, respectively (Figs. S2 and S4). For both batches of micrographs, global motion correction was first applied to all 32 frames using MOTIONCORR1 (Li et al., 2013), resulting global motion-corrected frame stacks and summed micrographs. The global motion-corrected stacks (the first 2 frames were discarded) were further processed by sub-region motion correction and dose weighting using MOTIONCORR2 (Zheng et al., 2017), generating summed micrographs with or without dose weighting. CTFFIND4 (Rohou and Grigorieff, 2015) was used to estimate the contrast transfer function (CTF) parameters and produce the CTF power spectrum on basis of summed micrographs from MOTIONCORR2. 2,565 and 3,678 micrographs were selected for further processing by screening summed micrographs and power spectra using SPIDER (Shaikh et al., 2008). Particles were auto-picked on summed micrographs from MOTIONCORR1 using RELION2.0 (Kimanius et al., 2016). For dataset of mTRPML1 amphipols, ~4,000 particles were manually picked in advance, and processed by 2D classification using RELION1.4 (Scheres, 2012). The resulting 2D averages were served as the templates for particle picking. For dataset of mTRPML1 nanodiscs, selected 2D averages from final 2D classification of mTRPML1 amphipols were used as the templates for particle auto-picking. For both batches of samples, two rounds of 2D classifications were performed to exclude noise and other bad particles. 783 K and 874 K particles from qualified 2D averages were selected for further 3D analysis. Ahead of 3D classification, a round of refinement was applied on the whole particles sets using RELION1.4. The particles were re-centered and processed by several rounds of 3D classification with tighter particle mask applied and C4 symmetry imposed using RELION1.4 (Figs. S2 and S4). To separate particles with different conformations and further exclude noise and bad particles more thoroughly, a cascade of 3D unsupervised or supervised classification with different combinations of parameters were tested.
For sample of mTRPML1 amphipols, three conformational states were identified, state 1 (~200 K, 26%), state 2 (~197 K, 25%) and state 3 (~167 K, 21%). Particles corresponding to state 2 and 3 were refined to 7.4 Å and 7.7 Å, respectively, with global mask applied and C4 symmetry imposed using RELION2.0. To further improve homogeneity and push resolution, further classification were performed onto particles corresponding to state 1, and the dominant group was refined to 5.8 Å finally. The map was sharpened using post-processing option of RELION with a B-factor of −400 Å2. For dataset of mTRPML1 nanodiscs, the particles were mainly composed by two sub-groups, particles with mTRPML1 inserting into nanodiscs (~242 K, 28%) and without nanodiscs (~376 K, 43 %). Though including less particles, the homogeneity and resolution of particle group with TRPML1 inserting into nanodiscs were obviously higher than particles without nanodiscs. The particle group with TRPML1 inserting into nanodiscs was further refined to 5.4 Å with global mask applied and C4 symmetry imposed using RELION2.0. The map was sharpened with a B-factor of −400 Å2. The local resolution map was calculated using ResMap (Kucukelbir et al., 2014), and displayed in Chimera (Pettersen et al., 2004).
Model of mTRPML1 transmembrane domain was predicted on I-TASSER server (Zhang, 2008). The predicted model of transmembrane domain and crystal structure of I-II linker (5TJA) were merged, docked into the two cryo-EM maps with higher resolutions (5.8 and 5.4 Å) from mTRPML1 amphipols and mTRPML1 nanodiscs sample respectively in Chimera, and manually adjusted in Coot (Emsley et al., 2010) to acquire the atom models of state 1 from mTRPML1 amphipols and mTRPML1 nanodiscs sample. Sequence alignment and secondary structure prediction of human TRPML1 were used to aid the model building. Model refinement was performed on the main chain of the two atom models using phenix.real_space_refine. Atom model of state 1 from mTRPML1 amphipols sample was docked into cryo-EM map of state 2 and 3, and each domain of the atom model were rigid-body fitted into the two density map respectively in Chimera (Pettersen et al., 2004) to acquire the atom models of state 2 and 3.
Quantification and statistical analysis
All reported resolutions are based on the gold-standard FSC = 0.143 criteria (Scheres and Chen, 2012), and the final FSC curve were corrected for the effect of a soft mask using high-resolution noise substitution (Chen et al., 2013). Final density maps were sharpened with a B-factor of −400 Å2 using RELION. Local resolution map was calculated using ResMap (Kucukelbir et al., 2014).
Data and software availability
The coordinates of the mTRPML1 in Amphipols and Nanodiscs have been deposited in the Worldwide Protein Data Bank (http://www.rcsb.org) with the accession codes 5YDZ, 5YE1, 5YE2 and 5YE5, respectively. The corresponding maps have been deposited in the Electron Microscopy Data Bank (http://emdatabank.org) with the accession codes EMD-6823, EMD-6824, EMD-6825, and EMD-6826, respectively.
Contact for reagent and resource sharing
For reagents in this paper or more information about resource sharing, please contact the corresponding author Maojun Yang (email@example.com)
We thank the Tsinghua University Branch of China National Center for Protein Sciences (Beijing) for providing the facility support. The computation was completed on the “Explorer 100” cluster system of Tsinghua National Laboratory for Information Science and Technology. This work was supported by funds from the National Key R&D Program of China (2017YFA0504600 and 2016YFA0501100, 2016YFA0500700 and 2017YFA0505800). The National Science Fund for Distinguished Young Scholars (31625008), the National Natural Science Foundation of China (Grant Nos. 21532004, 31570733, 31630087, and 31770936), and Beijing Natural Science Foundation (5172016).
cryo-EM, cryo-electron microscopy; MLIV, mucolipidosis type IV; PMD, polycystin-mucolipin domain; TRP, transient receptor potential; VGICs, voltage-gated ion channels; VSD, voltage-sensing domain
Compliance with Ethics Guidelines
Sensen Zhang, Ningning Li, Wenwen Zeng, Ning Gao, and Maojun Yang declare no competing financial interests. This article does not contain any studies with human or animal subjects performed by any of the authors.
MY. conceived, designed and supervised the project. S.Z. did the protein purification and detergent screening. N.L. W.Z. and S.Z. performed EM sample preparation, data collection. N.L. and N.G. performed the structural determination. All authors discussed the data of the manuscript and wrote the manuscript.
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