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Structural flexibility and functional interaction of mediator Cdk8 module

Protein & Cell volume 4, pages 911–920 (2013)Cite this article

Abstract

Mediator is a highly conserved large protein complex (25 proteins, >1000 kDa) and preeminently responsible for eukaryotic transcription, which contains a dissociable ‘Cdk8 module’. Although increasing evidence demonstrates that Cdk8 module plays both positive and negative roles in transcription regulation, the detailed structure, and subunit organization, molecular mechanism how it regulates transcription remain elusive. Here we used single-particle electron microscopy to characterize the structure and subunit organization of the Cdk8 module and illuminated the substantial mobility of the Med13 subunit results in the structural flexibility. The Cdk8 module interaction with core Mediator is concurrent with active transcription in vivo. An interaction with the Cdk8 module induces core Mediator into very extended conformation in vitro, which is presumed to be an active functional state of Mediator. Taken together, our results illuminated the detailed architecture of Cdk8 module, and suggested the Cdk8 module could positively regulate transcription by modulating Mediator conformation.

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Affiliations

  1. School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China

    Xuejuan Wang, Jianye Wang, Zhenrui Ding, Jinhua Ji, Qianqian Sun & Gang Cai

  2. Hefei National Laboratory for Physical Sciences at the Microscale, Center for Integrative Imaging, Hefei, 230026, China

    Gang Cai

Authors
  1. Xuejuan Wang
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  2. Jianye Wang
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  3. Zhenrui Ding
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  4. Jinhua Ji
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  5. Qianqian Sun
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  6. Gang Cai
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Correspondence to Gang Cai.

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These authors contributed equally to the work.

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Wang, X., Wang, J., Ding, Z. et al. Structural flexibility and functional interaction of mediator Cdk8 module. Protein Cell 4, 911–920 (2013). https://doi.org/10.1007/s13238-013-3069-y

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  • DOI: https://doi.org/10.1007/s13238-013-3069-y

Keywords

  • Mediator
  • Cdk8 kinase module
  • transcription regulation