Protein & Cell

, Volume 4, Issue 3, pp 220–230 | Cite as

Dynamic roles of angiopoietin-like proteins 1, 2, 3, 4, 6 and 7 in the survival and enhancement of ex vivo expansion of bone-marrow hematopoietic stem cells

  • Shahina Akhter
  • Md. Mashiar Rahman
  • Hyun Seo Lee
  • Hyeon-Jin Kim
  • Seong-Tshool Hong
Research Article

Abstract

Recent advances in hematopoietic stem cells (HSCs) expansion by growth factors including angiopoietin-like proteins (Angptls) have opened up the possibility to use HSCs in regenerative medicine. However, the unavailability of true in vitro HSCs expansion by these growth factors has limited the understanding of the cellular and molecular mechanism of HSCs expansion. Here, we report the functional role of mouse Angptls 1, 2, 3, 4, 6 and 7 and growth factors SCF, TPO, IGF-2 and FGF-1 on purified mouse bone-marrow (BM) LineageSca-1+(Lin-Sca-1+) HSCs. The recombinant retroviral transduced-CHO-S cells that secrete Angptls in serum-free medium were used alone or in combination with growth factors (SCF, TPO, IGF-2 and FGF-1). None of the Angptls stimulated HSC proliferation, enhanced or inhibited HSCs colony formation, but they did support the survival of HSCs. By contrast, any of the six Angptls together with saturating levels of growth factors dramatically stimulated a 3- to 4.5-fold net expansion of HSCs compared to stimulation with a combination of those growth factors alone. These findings lead to an understanding of the basic function of Angptls on signaling pathways for the survival as well as expansion of HSCs in the bone marrow niche.

Keywords

hematopoietic stem cells angiopoietin-like proteins growth factors survival ex vivo expansion cell culture 

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Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Shahina Akhter
    • 1
  • Md. Mashiar Rahman
    • 1
  • Hyun Seo Lee
    • 1
  • Hyeon-Jin Kim
    • 2
  • Seong-Tshool Hong
    • 1
  1. 1.Department of Microbiology and Genetics and Institute for Medical ScienceChonbuk National University Medical SchoolJeonjuSouth Korea
  2. 2.JINIS BDRD InstituteJINIS Biopharmaceuticals Co.JeollabukdoSouth Korea

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