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CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection

Protein & Cell volume 2, pages 463–469 (2011)Cite this article

Abstract

Murine gammaherpesvirus 68 (MHV-68), a member of the gammaherpesvirus family, replicates robustly in permissive cell lines and is able to infect laboratory mice. MHV-68 has emerged as a model for studying the basic aspects of viral replication and host-virus interactions of its human counterparts. Herpesvirus genome replication is mediated through a cis-element in the viral genome called the origin of lytic replication (oriLyt). A family of transcription factors, CCAAT/enhancer binding proteins (C/EBPs), assists in oriLyt-mediated DNA replication during gammaherpesvirus reactivation. In this study, we examined the role of C/EBPs in gammaherpesvirus DNA replication during de novo infection, using MHV-68 as a model. We found that C/EBP α and β bind to the CCAAT boxes in the MHV-68 oriLyt core region both in vitro and in vivo, as demonstrated by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. A dominant negative form of C/EBPs significantly impaired the lytic replication efficiency of MHV-68 on both the plasmid and genome levels in a replication assay, indicating that functional C/EBPs are required for maximal MHV-68 genome DNA replication. Collectively, our data demonstrate that C/EBPs interact with the oriLyt core region and play an important role in MHV-68 lytic DNA replication during de novo infection.

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Affiliations

  1. CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Jing Qi, Danyang Gong & Hongyu Deng

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100080, China

    Jing Qi & Danyang Gong

Authors
  1. Jing Qi
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  2. Danyang Gong
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  3. Hongyu Deng
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Correspondence to Hongyu Deng.

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Qi, J., Gong, D. & Deng, H. CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection. Protein Cell 2, 463–469 (2011). https://doi.org/10.1007/s13238-011-1060-z

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  • DOI: https://doi.org/10.1007/s13238-011-1060-z

Keywords

  • C/EBPs
  • murine gammaherpesvirus 68
  • oriLyt
  • lytic replication