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Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain

Protein & Cell volume 2, pages 470–476 (2011)Cite this article

Abstract

Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, the relationship between Hrs and citron kinase in HIV-1 production remains elusive. Here, we report that Hrs interacts with citron kinase via its FYVE domain. Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production. Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase. These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis.

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Affiliations

  1. Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Jiwei Ding & Guangxia Gao

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100039, China

    Jiwei Ding

  3. The University of North Carolina, Chapel Hill, NC, 27599-7295, USA

    Lishan Su

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  1. Jiwei Ding
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  2. Lishan Su
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  3. Guangxia Gao
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Correspondence to Guangxia Gao.

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Ding, J., Su, L. & Gao, G. Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain. Protein Cell 2, 470–476 (2011). https://doi.org/10.1007/s13238-011-1053-y

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  • DOI: https://doi.org/10.1007/s13238-011-1053-y

Keywords

  • citron kinase
  • Hrs
  • HIV-1 budding