Assessments of genetic diversity in Iranian flax populations using retrotransposon microsatellite amplification polymorphisms (REMAP) markers
Flax (Linum usitatissimum L., 2n = 30), is an annual self-pollinated crop. It is used in oil and fiber industries, and also valued for pharmaceutical and nutraceutical applications. Characterization of flax gene pool and evaluation of genetic diversity are crucial for germplasm conservation and breeding. In present study, eighteen retrotransposon microsatellite amplified polymorphism markers were used to evaluate the genetic diversity of cultivated flax within and among populations. Thirty samples of flax plants from three different climates and geographic regions in Iran were used for present investigation. Analysis of molecular variance test showed significant genetic difference (PhiPT = 0.595, P = 0.001) among the studied populations in L. usitatissimum. 41% of total genetic variability was due to within population diversity, whereas 59% was due to among population genetic differentiation. The PCoA plot and cluster analysis grouped plants of Saveh population in a cluster entirely separated from the other two populations and showed high genetic affinity between the two Shiraz and Orumieh populations. STRUCTURE plot identified two distinct gene pools for flax, so that the population of Saveh is different from other populations, but some degree of shared alleles occurred between them.
KeywordsFlax Genetic variability Germplasm REMAP Gene flow
We gratefully acknowledge Mr. Reza Assareh and Mrs. Bahareh Ghasemzadeh for their aids in Science and Research Branch of Islamic Azad University and Shahid Beheshti University.
- 1.Bickel CL, Gadani S, Lukacs M, Cullis CA. SSR markers developed for genetic mapping in flax (Linum usitatissimum L.). Res Rep Biol. 2011;2:23–9.Google Scholar
- 5.Cloutier S, Miranda E, Ward K, Radovanovic N, Reimer E, Walichnowski A, et al. Simple sequence repeat marker development from bacterial artificial chromosome end sequences and expressed sequence tags of flax (Linum usitatissimum L.). Theor Appl Genet. 2012;125:685–94.CrossRefPubMedPubMedCentralGoogle Scholar
- 7.Deng X, Long S, He D, Li X, Wang Y, Hao D, et al. Isolation and characterization of polymorphic microsatellite markers from flax (Linum usitatissimum L.). Afr J Biotechnol. 2011;10:734–9.Google Scholar
- 21.Križman M, Jakše J, Baričevič D, Javornik B, Prošek M. Robust CTAB-activated charcoal protocol for plant DNA extraction. Acta Agric Slov. 2006;87:427–33.Google Scholar
- 30.Sheidai M, Seif E, Nouroozi M, Noormohammadi Z. Cytogenetic and molecular diversity of Cirsium arvense (Asteraceae) populations in Iran. J Jpn Bot. 2012;87:193–205.Google Scholar
- 40.Zohary D, Pulses Hopf M. Domestication of plants in the old world: the origin and spread of cultivated plants in West Asia, Europe, and the Nile Valley. Oxford: Oxford Press; 2000. p. 316.Google Scholar