The Nucleus

, Volume 61, Issue 1, pp 69–73 | Cite as

Preliminary karyotype analysis of Amolops panhai and Sylvirana nigrovittata (Anura, Ranidae) from southern Thailand

  • Waranee Pradit
  • Chatmongkon Suwannapoom
  • Kotchaphan Bowonchairit
  • Kittisak Buddhachat
  • Opas Tantithakura
  • Siriwadee Chomdej
Original Article
  • 106 Downloads

Abstract

Karyotype and ideogram analyses of Somsak’s cascade frog (Amolops panhai) and dark-sided frog (Sylvirana nigrovittata), collected from Nakhon Si Thammarat Province, southern Thailand, were performed. Chromosomes were prepared from the intestinal epithelium and stained by conventional Giemsa staining. From this study, we found that A. panhai possesses the diploid chromosome number of 26 (2n = 26), while the chromosome number of S. nigrovittata is 24 (2n = 24). This is the first report on karyotype of A. panhai, containing 10 pairs of metacentric (m), 2 pairs of submetacentric (sm) and 1 pair of subtelocentric (st) chromosomes with the arm number of 52. The S. nigrovittata’s karyotype contains 12 pairs of chromosomes with the arm number of 48 in only the metacentric form. The karyotype formulae could be represented as 2n (26) = 10m + 2sm + 1st and 2n (24) = 12m for A. panhai and S. nigrovittata, respectively.

Keywords

Somsak’s cascade frog Dark-sided frog Karyotype Chromosome 

Notes

Acknowledgements

The financial support was granted from the National Research Council of Thailand. We thank Dr. Maslin Osathanunkul and Phuping Sucharitakul for their help and advice.

Compliance with ethical standards

Conflict of interest

There is no conflict of interests.

References

  1. 1.
    Amphibian Species of the World: an Online Reference. Version 6.0 (Dec. 22, 2016). Electronic Database accessible at http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA.
  2. 2.
    Campos JR, Martins IA, Haddad CF, Kasahara S. The karyotype of Holoaden luederwaldti (Anura, Strabomantidae), with report of natural triploidy. Folia Biol (Praha). 2012;58:144–50.Google Scholar
  3. 3.
    Cestari MM, Galetti PM. Chromosome evolution in the genus Serrasalmus and cytotaxonomic considerations about Serrasalminae (Characidae, Pisces). Rev Bras Genet. 1992;15:555–67.Google Scholar
  4. 4.
    Chen L, Murphy RW, Lathrop A, Ngo A, Orlov NL, Ho CT, et al. Taxonomic chaos in Asian ranid frogs: an initial phylogenetic resolution. Herpetol J. 2005;15:231–43.Google Scholar
  5. 5.
    Chuaynkern Y, Chuaynkern C. Checklist of amphibians in Thailand. J Wildl Thail. 2012;19:163–211.Google Scholar
  6. 6.
    Dever JA, Fuiten AM, Konu Ö, Wilkinson JA. Cryptic torrent frogs of Myanmar: an examination of the Amolops marmoratus species complex with the resurrection of Amolops afghanus and the identification of a new species. Copeia. 2012;2012:57–76.CrossRefGoogle Scholar
  7. 7.
    Frost DR, Grant T, Faivovich J, Bain RH, Haas A, Haddad CFB, et al. The amphibian tree of life. Bull Am Mus Nat Hist. 2006;297:370.CrossRefGoogle Scholar
  8. 8.
    Frost DR, Grant T, Faivovich J, Bain RH, Haas A, Haddad CFB, et al. Is the amphibian tree of life really fatally flawed? Cladistics. 2008;24:385–95.CrossRefGoogle Scholar
  9. 9.
    Jiang K, Yan F, Suwannapoom C, Chomdej S, Che J. A new species of the genus Leptolalax (Anura: Megophryidae) from northern Thailand. Asian Herpetol Res. 2013;4:100–8.CrossRefGoogle Scholar
  10. 10.
    Kuramoto M, Yong H-S. Karyotypes of several frog species from Peninsular Malaysia. Herpetologica. 1992;48:434–8.Google Scholar
  11. 11.
    Levan A, Fredga K, Sandberg AA. Nomenclature for centromeric position on chromosomes. Hereditas. 1964;52:201–20.CrossRefGoogle Scholar
  12. 12.
    Li B. An analysis of the karyotype of Amolops wuyiensis. Acta Herpetol Sin. 1987;6:42–5.Google Scholar
  13. 13.
    Li S, Hu J. The karyotypes, C-banding and Ag-NORs on two species of genus Hylarana from Xishuang Banna of Yunnan. Hereditas (Beijing). 1994;16:21–3.Google Scholar
  14. 14.
    Li S. Study on the karyotype, C-banding and Ag-NORs of Amolops mantzorum from Jingdong County of Yunnan Province. Chin J Zool. 1995;30:12–5.Google Scholar
  15. 15.
    Liu W, Yang D. A comparative study on karyotype and chromosome banding of three species of Amolops from southeastern China. Zool Res. 1994;15:158–65.Google Scholar
  16. 16.
    Liu W, Yang D. A new species of Amolops (Anura: Ranidae) from Yunnan, China, with a discussion of karyological diversity in Amolops. Herpetologica. 2000;56:231–8.Google Scholar
  17. 17.
    Matsui M, Ota H, Lau MW, Bogadek A. Cytotaxonomic studies of three ranid species (Amphibia: Anura) from Hong Kong. Jpn J Herpetol. 1995;16:12–8.CrossRefGoogle Scholar
  18. 18.
    Matsui M, Nabhitabhata J. A new species of Amolops from Thailand (Amphibia, Anura, Ranidae). Zool Sci. 2006;23:727–32.CrossRefPubMedGoogle Scholar
  19. 19.
    Rujirawan A, Stuart BL, Aowphol A. A new tree frog in the genus polypedates (Anura: Rhacophoridae) from southern Thailand. Zootaxa. 2013;3702:545–65.CrossRefPubMedGoogle Scholar
  20. 20.
    Sakamoto Y, Zacaro AA. LEVAN, an ImajeJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes. Initial version. An open source Java plugin distributed over the internet from http://rsbweb.nih.gov/ij/. 2009.
  21. 21.
    Siqueira S, Aguiar O, Pansonato A, Giaretta AA, Strüssmann C, Martins I, et al. The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species. Genet Mol Biol. 2009;32:470–6.CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Supaprom T, Udomsirichakorn K, Chantree P. Chromosome complements and standard karyotypes of two frog species: Occidozygo lima (Gravenhorst) 1829 and Rana cubitalis Smith 1917. The 24th Congress on Science and Technology of Thailand; 1998 19th–21st October; Bangkok.Google Scholar
  23. 23.
    Wu G, Zhao E. Two rare karyotypes of anurans, the karyotypes of Staurois mantzorum and S. liangshanensis. Acta Herpetol Sin. 1984;3:5–10.Google Scholar
  24. 24.
    Wu G, Zhao E. Preliminary studies on karyotypes of the genus Amolops of the Hengduan mountains. Acta Herpetol Sin. 1985;4:276–82.Google Scholar
  25. 25.
    Yang Y. Karyological studies on amphibians in China. Asiat Herpetol Res. 2004;10:298–305.Google Scholar
  26. 26.
    Zhang C, Yuan S, Xia Y, Zeng X. Species delimitation of Amolops kangtingensis. Sichuan J Zool. 2015;34:801–9.Google Scholar

Copyright information

© Archana Sharma Foundation of Calcutta 2017

Authors and Affiliations

  • Waranee Pradit
    • 1
  • Chatmongkon Suwannapoom
    • 2
  • Kotchaphan Bowonchairit
    • 3
  • Kittisak Buddhachat
    • 4
  • Opas Tantithakura
    • 5
  • Siriwadee Chomdej
    • 3
  1. 1.Science and Technology Research InstituteChiang Mai UniversityChiang MaiThailand
  2. 2.Department of Fishery, School of Agriculture and Natural ResourcesUniversity of PhayaoPhayaoThailand
  3. 3.Department of Biology, Faculty of ScienceChiang Mai UniversityChiang MaiThailand
  4. 4.Department of Biology, Faculty of ScienceNaresuan UniversityPhitsanulokThailand
  5. 5.School of ScienceWalailak UniversityNakorn Si ThammaratThailand

Personalised recommendations