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Karyotypic analysis of parents and F1 hybrids of intra-specific crosses in Momordica charantia L.

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Abstract

A detailed karyotypic study of the most commonly cultivated and the medicinally important species Momordica charantia var. charantia along with its wild variety M. charantia var. muricata (two accessions) and their F1 hybrids were analysed to find out the interrelationships of the taxa. It has been observed that the somatic chromosome number of the parents and hybrids was 2n = 22, and they can be differentiated by their karyotype formula and quantitative parameters. The parents involved in the cross combinations belonged to the karyotpic category 4A whereas the F1 hybrids were grouped under the karyotype 3A. The TF% and intra chromosomal asymmetry indices along with the asymmetrical karyotype pointed out the advanced nature of the cultivated variety. Chromosome polymorphism was observed both at the intraspecific and intravarietal level. The Principal Component Analysis suggested that the TF%, total chromosome length and short arm length were the karyotypic features that distinguished the parents and the hybrids. Unweighed Pair Group Method with Arithmetic Mean (UPGMA) dendrogram differentiated the wild accessions at the intra varietal level. Close relationship of the F1 hybrids with the wild accession W2 was also evident. UPGMA clustering indicates that the wild accession W1 may be the immediate ancestor of the cultivated variety. The karyotypic data can be utilized to differentiate the wild and the two cultivated varieties of Momordica charantia and to clarify their intra specific relationships. The findings may provide useful information on the systematics of Momordica charantia.

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References

  1. Abraham Z, Prasad PN. A system of chromosome classification and nomenclature. Cytologia. 1983;48:95–101.

    Article  Google Scholar 

  2. Anuradha T, Reddy GLK. D2 analysis in sesame (Sesamum indicum L.). J Oil Seeds Res. 2005;22:174–5.

    Google Scholar 

  3. Beevy SS. Biosystematics studies on the south Indian Cucurbitaceae. Ph.D.thesis. 1994; Trivandrum: Kerala University.

  4. Beevy SS, Bai HN. Intra specific diversity analysis in Momordica charantia L.(Cucurbitaceae). International Symposium and XXII Annual Conference of Indian Association for Angiosperm Taxonomy on Innovative Prospects in AngiospermTaxonomy (ISIPAT- 2012). 2012; Abstract, Sant Gadge Baba Amravati University, pp 96.

  5. Bai HN, Beevy SS. Characterization of intra specific F1 hybrids of Momordica charantia L. based on morphology, cytology and palynology. Cytologia. 2012;77:1–10.

    Google Scholar 

  6. Bhaduri PN, Bose PC. Cytogenetical investigations in some common cucurbits, with special reference to fragmentation of chromosome as a physical basis of speciation. J Genet. 1947;48:237–56.

    Article  PubMed  CAS  Google Scholar 

  7. Bharathi LK, Munshi AD, Behera TK, Das AB. Cytotaxonomical analysis of M.charantia L. species of Indian occurrence. J Cytogenetics. 2011;90(1):21–9.

    CAS  Google Scholar 

  8. Bharathi LK, Vinod, Munshi AD, Behera TK, Chandrashekaran S, Kattukunnel J. Cytomorphological evidence for segmental allopolyploid origin of teasle gourd(Momordica subangulata subsp. renigera). Euphytica. 2010;176:79–85.

    Article  Google Scholar 

  9. Bharathi LK, Vinod, Das AB, Ghosh N, Behera TK, Nak G, et al. Cytomorphological and molecular characterization of inter specific F1 hybrid of Momordica dioicaRoxb. X Momordica subangulata subsp. Renigera (G. Don) de Wilde. Afr J Agric Res. 2011;6:2982–90.

    Google Scholar 

  10. Chakravarthy HL. Momordica charantia L. In: Fascicles of flora India. Cucurbitaceae. 1990; Botanical Survey of India, Culcutta pp. 87–95.

  11. Gawali CW, Bhoitre KD, Pardeshi SR. Genetic divergence in sesame (Sesamum indicum L.). Res Crops. 2006;7:758–9.

    Google Scholar 

  12. Huziwara Y. Karyotype analysis in some genera of Compositae VIII. Further studies in the chromosomes of Aster. Am J Bot. 1962;49:116–9.

    Article  Google Scholar 

  13. Imazu T, Fujishita N. Cucumbers. In: Kihara H, editor. Land and crops of Nepal Himalaya 1952–53 Vol.II. Fauna and Flora research society. Kyoto: Kyoto University; 1959. p. 218–28.

    Google Scholar 

  14. Kazem Y, Houshmand S, Madani B, Gómez PM. Karyotypic studies in Iranian almond species. Caryologia. 2010;63:117–23.

    Google Scholar 

  15. Kho YO, den Nijs APM, Franken J. Intra specific hybridization in Cucumis L. The crossability of species, an investigation of in vivo pollen tube growth. Euphytica. 1980;29:661–72.

    Article  Google Scholar 

  16. Malik A, Srivastava AK. Karyotypic analysis of different populations of Carthamus tinctorius Linnaeus (Asteraceae). Comp Cytogen. 2009;3:51–5.

    Google Scholar 

  17. Maria FJ, Lauhinghouse D, Silva ACFD, Tedesco SB. Variability of the chromosomal number and meiotic behaviour in populations of Bidens pilosa L. (Asteraceae) from southern Brazil. Caryologia. 2008;61:164–9.

    Google Scholar 

  18. Mukherjee A, Roy SC. Karyotype analysis of five species of Allium. Indian J Funda Appl Life Sci. 2012;2:374–83.

    Google Scholar 

  19. Naruhashi N, Iwatsubo Y. Cytotaxonomic study on two putative hybrids in the genus Duchesnea (Rosaceae). Bot Mag Tokyo. 1991;104:137–43.

    Article  Google Scholar 

  20. Satija CK, Vikal Y. Crossability and morphological studies among wild and cultivated species of Vigna. Cytol Genet. 1993;28:173–9.

    Google Scholar 

  21. Shan F, Yan G, Plummer JA. Karyotype evolution in the genus Boronia (Rutaceae). Bot J Linn Soc. 2003;142:309–20.

    Article  Google Scholar 

  22. Sharma AK, Sharma A. Recent advances in the study of chromosomal alterations with relation to speciation. Bot Rev. 1959;25:514–44.

    Article  Google Scholar 

  23. Sheidai M, Dokhanchei A, Noormohammadi Z. Karyotype and chromosome pairing analysis in some Iranian upland cotton (Gossypium hirsutum) cultivars. Cytologia. 2008;73:275–81.

    Article  Google Scholar 

  24. Stebbins GL. Chromosomal evolution in higher plants. London: Edward Arnold Ltd; 1971.

    Google Scholar 

  25. Stebbins GL. Flowering plants evolution above the species level. Massachusetts: Harvard University Press Cambridge; 1974.

    Google Scholar 

  26. Trivedi RN. Cytogenetical studies in triploid M.dioica. Proc 65th Indian Sci Congr. 1978;Part 3:116.

  27. Trivedi RN, Roy RP. Cytogenetics of Momordica charantia and its polyploids. Cytologia. 1973;38:317–25.

    Article  Google Scholar 

  28. Ujjainkar VV, Datke SB, Rathod TH. Genetic divergence studies in sesame (Sesamum indicumL.). Ann Plant Physiol. 2002;16:18–22.

    Google Scholar 

  29. Wang LQZ, Hood E, Wang M, Scalzo R. Chromosome karyotypes of Echinacea angustifolia var. angustifolian and E. Purpurea. Hort Sci. 2004;39:368–70.

    Google Scholar 

  30. Yazdani M, Sarrami M, Zeinali H. Karyotype study of seven safflower (Carthamus tinctorious L.) populations native to Iran. Nucleus. 2013;56:31–6.

    Article  Google Scholar 

  31. Zaman MY, Alam SS. Karyotype diversity in three cultivars of M.charantia L. Cytologia. 2009;74:473–8.

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the Department of Science and Technology for the financial support and Dr. Ashalatha S. Nair, Professor & Head, Department of Botany, University of Kerala, for providing the facilities.

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Authors and Affiliations

  1. Department of Botany, University of Kerala, Kariavattom, Thiruvananthapuram, 695 581, India

    S. Suhara Beevy & N. Haseena Bai

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  1. S. Suhara Beevy
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  2. N. Haseena Bai
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Correspondence to S. Suhara Beevy.

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Beevy, S.S., Bai, N.H. Karyotypic analysis of parents and F1 hybrids of intra-specific crosses in Momordica charantia L.. Nucleus 56, 99–105 (2013). https://doi.org/10.1007/s13237-013-0087-3

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