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Sodium azide (NaN3) induced genetic variation of Psoralea corylifolia L. and analysis of variants using RAPD markers

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Abstract

The effect of sodium azide (NaN3) was studied in Psoralea corylifolia L (2n = 22). Three distinct phenotypic variants one at 0.50 % and two at 0.75 % concentrations of NaN3 were selected for analysis. Besides morphological variations in the form of size, height, number of branches, number of flowers and seeds, cytological anomalies in the form of pollen fertility, univalents, multivalents, laggards, bridges, micronuclei, were also observed in all variants and the anomalies showed decreasing order at the final stages of both meiosis and mitosis. These variants were further analysed using randomly amplified polymorphic DNA (RAPD) technique, which differentiated the variants genotypically, on the basis of occurrence of polymorphic DNA bands. The percentage of polymorphism was found to be highest (70 %) using primer OPH-12 followed by OPC-02 having 66.66 % of polymorphism. Among 31 DNA bands 19 were found to be polymorphic which showed great variation created by NaN3 in the genome of the experimental plant. Therefore the above concentrations of sodium azide are best suited for the induction of genetic variations in Psoralea corylifolia.

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Authors and Affiliations

  1. Cytogenetics and mutation Breeding Laboratory: Department of Botany Aligarh, Muslim University Aligarh, Aligarh, 202002, India

    Towseef Mohsin Bhat, Mohd Yunus Khalil Ansari,  Alka & Rumana Aslam

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  1. Towseef Mohsin Bhat
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  2. Mohd Yunus Khalil Ansari
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  3. Alka
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Correspondence to Towseef Mohsin Bhat.

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Bhat, T.M., Ansari, M.Y.K., Alka et al. Sodium azide (NaN3) induced genetic variation of Psoralea corylifolia L. and analysis of variants using RAPD markers. Nucleus 55, 149–154 (2012). https://doi.org/10.1007/s13237-012-0069-x

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