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Evaluation of pollen and productive parameters, their interrelationship and clustering of eight Corchorus spp. (Tiliaceae)

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Abstract

Pollen characteristics namely, pollen fertility (2% acetocarmine), viability (stain tests: Aniline blue, Lugol’s iodine, x-gal and Amido black), pollen production/flower (direct count using improved Neubauer Hemocytometer), pollen nuclei composition (following DAPI staining - 4′,6-diamidino-2-phenylindole) and pollen size along with parameters like capsule/plant, capsule length and seed set/capsule were studied in two cultivated (Corchorus capsularis L. and C. olitorius L.) and six wild (C. aestuans L., C. fascicularis Lamk., C. pseudocapsularis L., C. pseudoolitorius I. and Z., C. tridens L. and C. trilocularis L.) species of Corchorus under the Experimental field conditions of Kalyani University (West Bengal plains; latitude 22°50′–24°11′ N, longitude 88°09′–88°48′ E, elevation 48 ft above sea level, sandy loamy soil, organic carbon 0.76%, soil pH 6.85). Results obtained were statistically analyzed and the information accumulated may provide necessary impetus for formulating efficient breeding strategies and crop improvement. Further, clustering of the species on the basis of the attributes analyzed by UPGMA will be helpful for designing crossing experiments.

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Acknowledgement

The Research is grant aided by University Grants Commission (India).

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Authors and Affiliations

  1. Department of Botany, Cytogenetics and Plant Breeding Section, University of Kalyani, Kalyani, 741235, West Bengal, India

    Aninda Mandal, Animesh K. Datta & Arnab Bhattacharya

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  1. Aninda Mandal
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  2. Animesh K. Datta
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Correspondence to Animesh K. Datta.

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Mandal, A., Datta, A.K. & Bhattacharya, A. Evaluation of pollen and productive parameters, their interrelationship and clustering of eight Corchorus spp. (Tiliaceae). Nucleus 54, 147–152 (2011). https://doi.org/10.1007/s13237-011-0044-y

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