The Nucleus

, Volume 53, Issue 1–2, pp 51–54 | Cite as

Molecular cytogenetics of Astyanax ribeirae (Teleostei, Characidae), an endemic characin of the Atlantic rainforest

  • Karine Frehner KavalcoEmail author
  • Rubens Pazza
  • Lurdes Foresti de Almeida-Toledo
Original Article


Astyanax ribeirae is a characin fish endemic to Ribeira de Iguape river basin, Brazil. Karyotypic analysis of the specimen from distinct geographic regions depicted similar karyotypic features, i.e. 2n = 50 constituting the 4 M + 10SM + 6ST + 30A and a fundamental number (FN) equal to 70. Further analysis on linear differentiation using silver nitrate staining, c-banding and rDNA localization revealed single NOR on ST/A chromosomes, heterochromatin blocks in the pericentromeric region of some chromosomes, and four 18S rDNA sites and six 5S rDNA sites, all located on ST/A chromosomes. FISH with As-51 satellite DNA probe presented no homology signals in chromosomes of A. ribeirae, indicating uniqueness of species within the genus Astyanax. The chromosomal and molecular cytogenetic data obtained on A. ribeirae specimens collected from Atlantic rainforest areas are discussed to elucidate relationship between this species and other species in the genus vis-à-vis chromosomal evolution of the group. This is the first report on cytogenetic analysis of Astyanax ribeirae.


Characiformes Ecological genetics FISH Karyotype Chromosome evolution 



The authors wish to thank LR Malabarba LR and V Bertaco for the taxonomical identification, CE Lopes for the help with the samples, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for the financial support. The experiments comply with the current laws in Brazil.


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Copyright information

© Archana Sharma Foundation of Calcutta 2010

Authors and Affiliations

  • Karine Frehner Kavalco
    • 1
    Email author
  • Rubens Pazza
    • 1
  • Lurdes Foresti de Almeida-Toledo
    • 2
  1. 1.Laboratório de Genética Ecologica e EvolutivaUniversidade Federal de ViçosaRio ParanaíbaBrazil
  2. 2.Instituto de Biociências, Departamento de Genética e Biologia EvolutivaUniversidade de São PauloSão PauloBrazil

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