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Bioactive polymers for cosmetics: unleashing the potential of Chromohalobacter canadensis 28

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Abstract

The growing demand for natural products in the cosmetic industry has led to increased interest in natural polysaccharides that offer biocompatibility, biodegradability, and bioactivity. Microorganisms have emerged as a promising source for large-scale production of these polysaccharides under controlled conditions. Among them, the halophilic bacterium Chromohalobacter canadensis 28 stands out as it produces an exopolymer (Cc EP) consisting of exopolysaccharides (EPS) and poly-gamma-glutamic acid (γ-PGA), making it the first halophilic microorganism known for PGA production. This study explores the potential of crude Cc EP and purified γ-PGA for improving the skin barrier, stimulating collagen and hyaluronic acid production, and facilitating wound closure. Through in-vitro experiments using human epidermal keratinocyte cells (HaCaT) and dermal fibroblast cells (PCS-201-012), both the exopolymers were found to exhibit positive effects on cellular proliferation and the expression of genes related to collagen, hyaluronic acid, involucrin, and filaggrin. Furthermore, in-vitro scratch models demonstrated their ability to enhance wound closure. Based on these promising findings, we propose that Cc EP and purified γ-PGA hold potential as active ingredients in cosmeceutical products. Their ability to promote cell proliferation, improve collagen and hyaluronic acid expression, and enhance wound closure makes them valuable assets for skincare applications.

Graphical abstract

Production and effect of Chromohalobacter canadensis 28 exopolymer on human skin cells

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All data generated or analyzed during this study are included in this published article.

References

  1. I. Aranaz et al., Polymers 10, 213 (2018)

    Article  PubMed  PubMed Central  Google Scholar 

  2. B. Haney, Springer 2020.

  3. M.E. Hasköylü and E.T. Öner, in The Book of Fructans, Elsevier2023, pp 275–294.

  4. J. Kottner, et al., in Innovations and Emerging Technologies in Wound Care, Elsevier2020, pp 183–196.

  5. K. Vig et al., Int. J. Mol. Sci. 18, 789 (2017)

    Article  PubMed  PubMed Central  Google Scholar 

  6. S. Agrawal et al., Phytochem. Lett. 23, 15 (2018)

    Article  CAS  Google Scholar 

  7. Y. Gu et al., Ageing Res. Rev. 59, 101036 (2020)

    Article  CAS  PubMed  Google Scholar 

  8. V. Gupta et al., Gels 8, 173 (2022)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. P. Morganti et al., Cosmetics 8, 65 (2021)

    Article  CAS  Google Scholar 

  10. C.G. Sotelo et al., Cosmetics 8, 48 (2021)

    Article  CAS  Google Scholar 

  11. A. Adrien et al., Carbohyd. Polym. 157, 1306 (2017)

    Article  CAS  Google Scholar 

  12. Z.I. Gazitaeva, et al., Clin. Cosmet. Investig. Dermatol., 11 (2017).

  13. J.K. Nguyen, N. Masub, J. Jagdeo, J. Cosmet. Dermatol. 19, 1555 (2020)

    Article  PubMed  Google Scholar 

  14. I. Priyan Shanura Fernando et al., Crit. Rev. Biotechnol. Rev. Biotechnol. 39, 99 (2019)

    Article  CAS  Google Scholar 

  15. M.V. Setshego et al., BMC Complement. Med. Ther. 20, 1 (2020)

    Article  Google Scholar 

  16. F. Freitas, V.D. Alves, and M.A. Reis, in Polysaccharides, Springer2015, pp 2017–2043.

  17. S.S. Rao, Athmika, and P. Rekha, in Biopolymers: Recent Updates, Challenges and Opportunities, Springer2022, pp 223–244.

  18. E.T. Öner, L. Hernández, J. Combie, Biotechnol. Adv. 34, 827 (2016)

    Article  PubMed  Google Scholar 

  19. N. Radchenkova et al., Appl. Microbiol. Biotechnol. 102, 4937 (2018)

    Article  CAS  PubMed  Google Scholar 

  20. N. Radchenkova et al., Microorganisms 8, 1935 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. P. Nair, G.R. Navale, M.S. Dharne, Biomass Convers. Biorefinery 13, 4555 (2023)

    Article  Google Scholar 

  22. N. Ben-Zur and D.M. Goldman, Cosmetics and toiletries, 122 (2007).

  23. D.P. Tiwari et al., FEMS Microbiol. Lett. Microbiol. Lett. 365, fny248 (2018)

    CAS  Google Scholar 

  24. G.A. Birrer, A.-M. Cromwick, R.A. Gross, Int. J. Biol. Macromol. 16, 265 (1994)

    Article  CAS  PubMed  Google Scholar 

  25. J. Feng et al., Metab. Eng. 32, 106 (2015)

    Article  CAS  PubMed  Google Scholar 

  26. G.H. Ho et al., J. Chin. Chem. Soc. 53, 1363 (2006)

    Article  CAS  Google Scholar 

  27. W.F. Liu et al., Mater. Sci. Forum Trans. Tech. Publ. 932, 124–128 (2018)

    Article  Google Scholar 

  28. J. Meerak et al., J. Gen. Appl. Microbiol. 54, 159 (2008)

    Article  CAS  PubMed  Google Scholar 

  29. K. Shimizu, H. Nakamura, M. Ashiuchi, Appl. Environ. Microbiol. 73, 2378 (2007)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. H. Zwartouw, H. Smith, Biochem. J. 63, 437 (1956)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. N. Radchenkova et al., Eng. Life Sci. 20, 357 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. M. DuBois et al., Anal. Chem. 28, 350 (1956)

    Article  CAS  Google Scholar 

  33. B.I. Pinto, et al., JoVE (Journal of Visualized Experiments), e58838 (2019).

  34. M. Erginer et al., Int. J. Biol. Macromol. 240, 124418 (2023)

    Article  CAS  PubMed  Google Scholar 

  35. Z. Li et al., RSC Adv. 7, 11085 (2017)

    Article  CAS  Google Scholar 

  36. A. Ogunleye et al., Microbiology 161, 1 (2015)

    Article  CAS  PubMed  Google Scholar 

  37. Q. Xu et al., Clin Exp Allergy 36, 94 (2006)

    Article  CAS  PubMed  Google Scholar 

  38. W. Liu et al., Int. J. Biol. Macromol. 65, 446 (2014)

    Article  CAS  PubMed  Google Scholar 

  39. Z. Guo et al., Bioorg. Med. Chem. Lett. 15, 4600 (2005)

    Article  CAS  PubMed  Google Scholar 

  40. C. Wang, D. Hua, C. Yan, Ind. Crops Prod. 70, 427 (2015)

    Article  CAS  Google Scholar 

  41. N.T. Quach et al., World J. Microbiol. Biotechnol. 38, 173 (2022)

    Article  CAS  PubMed  Google Scholar 

  42. J.M. Lee et al., Int. J. Biol. Macromol. 153, 616 (2020)

    Article  CAS  PubMed  Google Scholar 

  43. S.R. Varma et al., J. Tradit. Complement. Med. 9, 5 (2019)

    Article  PubMed  Google Scholar 

  44. H. Iizuka et al., J. Dermatol. 31, 271 (2004)

    Article  PubMed  Google Scholar 

  45. J. Jean et al., J. Dermatol. Sci. 53, 19 (2009)

    Article  CAS  PubMed  Google Scholar 

  46. D. Gutowska-Owsiak et al., Exp. Dermatol. 21, 104 (2012)

    Article  CAS  PubMed  Google Scholar 

  47. M.-K. Kang et al., Int. J. Mol. Sci. 22, 6137 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. K. Motoyama, et al., Occupational and Environmental Skin Disorders: Epidemiology, Current Knowledge and Perspectives for Novel Therapies, 79 (2018).

  49. M. Furukawa et al., J. Dermatol. Sci. 98, 137 (2020)

    Article  CAS  PubMed  Google Scholar 

  50. R. Miyake et al., J. Dermatol. 49, 1148 (2022)

    Article  CAS  PubMed  Google Scholar 

  51. J. Rossert and B. de Crombrugghe, in Principles of bone biology, Elsevier 2002, pp. 189–XVIII.

  52. R. Gref et al., Sci. Rep. 10, 16883 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. M. Gunnarsson et al., J. Colloid Interface Sci. 604, 480 (2021)

    Article  CAS  PubMed  Google Scholar 

  54. M.M. van Mierlo et al., Clin Exp Allergy 51, 1510 (2021)

    Article  PubMed  PubMed Central  Google Scholar 

  55. Y. Zhao et al., J. Mech. Behav. Biomed. Mater. 103, 103552 (2020)

    Article  CAS  PubMed  Google Scholar 

  56. J.-Y. Berthon et al., Free Radic. Res. 51, 555 (2017)

    Article  CAS  PubMed  Google Scholar 

  57. G. Bou-Gharios, D. Abraham, and B. de Crombrugghe, in Principles of bone biology, Elsevier2020, pp. 295–337.

  58. H.K. Kim, Molecules 21, 70 (2016)

    Article  PubMed  PubMed Central  Google Scholar 

  59. C.-Y. Su et al., J. Dental Sci. 14, 192 (2019)

    Article  Google Scholar 

  60. J. Hua et al., Mater. Sci. Eng. C 61, 879 (2016)

    Article  CAS  Google Scholar 

  61. M. Zhang et al., React. Funct. Polym. 122, 131 (2018)

    Article  CAS  Google Scholar 

  62. Z. Uddin et al., Macromol. Biosci. 20, 2000132 (2020)

    Article  CAS  Google Scholar 

  63. R. Wang et al., ACS Appl. Mater. Interfaces 11, 37502 (2019)

    Article  CAS  PubMed  Google Scholar 

  64. S. Datta et al., Int. J. Polym. Mater. Polym. Biomater.Polym. Mater. Polym. Biomater. 69, 65 (2020)

    Article  CAS  Google Scholar 

  65. A. Sun et al., NPG Asia Mater. 12, 25 (2020)

    Article  CAS  Google Scholar 

  66. Y. Fang et al., Eur. Polymer J. 116, 30 (2019)

    Article  CAS  Google Scholar 

  67. C.T. Tsao et al., Carbohyd. Polym. 84, 812 (2011)

    Article  CAS  Google Scholar 

  68. L. Zhu et al., Mater. Sci. Eng. C 129, 112374 (2021)

    Article  CAS  Google Scholar 

  69. J. Zheng et al., Int. J. Pharm. 450, 296 (2013)

    Article  CAS  PubMed  Google Scholar 

  70. Y. Ouyang, J. Zhao, S. Wang, Int. J. Biol. Macromol. 227, 505 (2023)

  71. R. Wang et al., Adv. Func. Mater. 32, 2113034 (2022)

    Article  CAS  Google Scholar 

  72. Z. Chen et al., Int. J. Biol. Macromol. 225, 1235 (2023)

    Article  CAS  PubMed  Google Scholar 

  73. Y. Wang et al., Nanomaterials 8, 324 (2018)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. W.-C. Liu et al., Mater. Sci. Eng. C 101, 630 (2019)

    Article  CAS  Google Scholar 

  75. M.H. Sung, et al., Google Patents (2014)

  76. S.S. Selvi, M.E. Hasköylü, and E.T. Öner, in Tissue Eng., Apple Academic Press 2022, pp. 159–202.

  77. N. Sahiner et al., Int. J. Biol. Macromol. 82, 150 (2016)

    Article  CAS  PubMed  Google Scholar 

  78. W. Zhang et al., Adv. Healthcare Mater. 6, 1700121 (2017)

    Article  Google Scholar 

  79. L. Fan et al., RSC Adv. 2, 4110 (2012)

    Article  CAS  Google Scholar 

  80. S. Iriyama et al., Sci. Rep. 12, 795 (2022)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  81. W. Cao, Y. Feng, Biol. Res. (2019). https://doi.org/10.1186/s40659-019-0260-5

    Article  PubMed  PubMed Central  Google Scholar 

  82. Y. Kawano et al., Pharmaceuticals 14, 301 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  83. A. Pilloni, et al., J. Periodontol. 94(3), 354 (2023)

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Funding

This study was funded by a Bilateral Agreement between Bulgarian Academy of Sciences (BAS) Bulgaria (Grant Number B02/26) and TUBITAK, Turkey (Grant Number 118M797).

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Authors and Affiliations

  1. Institute of Nanotechnology and Biotechnology, Istanbul University-Cerrahpaşa, Istanbul, Turkey

    Merve Erginer

  2. Department of Bioengineering, Istanbul Medeniyet University, 34700, Istanbul, Turkey

    Songül Yaşar Yıldız

  3. Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

    Margarita Kambourova

  4. Department of Bioengineering, Industrial Biotechnology and Systems Biology (IBSB), Marmara University, Istanbul, Turkey

    Ebru Toksoy Öner

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  1. Merve Erginer
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  2. Songül Yaşar Yıldız
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  3. Margarita Kambourova
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  4. Ebru Toksoy Öner
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Contributions

MEH, SYY, and ETO conceived and designed the study. MEH and SYY conducted experiments. MEH, SYY, MK, and ETO analyzed the data and wrote the manuscript. ETO managed the laboratory and provided the resources and funding. All the authors have read and approved the manuscript.

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Correspondence to Songül Yaşar Yıldız.

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Erginer, M., Yaşar Yıldız, S., Kambourova, M. et al. Bioactive polymers for cosmetics: unleashing the potential of Chromohalobacter canadensis 28. Macromol. Res. 32, 325–336 (2024). https://doi.org/10.1007/s13233-023-00236-4

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