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Direct Solvent-Free Modification of the Inner Wall of the Microchip for Rapid DNA Extraction with Enhanced Capturing Efficiency

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Abstract

Nucleic acid (NA) extraction and purification are one of the crucial steps for NA-based molecular diagnosis. However, the currently developed methods are still suffering from many issues including long process time, complicated steps, requirement of trained personnel and potential inhibition caused by chaotropic agents and/ or residual reagents. Herein, a surface-modified NA extraction microchip (SNC) is newly fabricated by introducing poly(2-dimethylaminomethyl styrene) (pDMAMS) film engaged directly on the microchip surface via initiated chemical vapor deposition (iCVD) process. The positively charged SNC inner surface could directly capture the negatively charged NA efficiently and its performance is confirmed by fluorescence microscopy and X-ray photoelectron spectroscopy. The developed SNC exhibits the deoxyribonucleic acid (DNA) capture efficiency higher than 92% regardless of initial DNA concentration in range of 20 ng/µL to 0.01 ng/µL. With this versatile DNA-capturing surface, the genomic DNAs of Escherichia Coli O157:H7 (E. coli O157:H7) is successfully extracted directly from cell lysate in the SNC with higher than 90% of efficiency within 30 min. The extraction time can be reduced to at least of 10 min maintaining extraction efficiency higher than 50%. Furthermore, the genomic DNAs are directly extracted using the SNC without loss from various real samples including juice, milk and blood serum. The proposed SNC enables us to perform an one-step NA extraction for molecular diagnosis and has the potential to be integrated into a micro-total analysis in the fields of point-of-care diagnosis.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Korea

    Yunho Choi, Eunjung Lee & Sung Gap Im

  2. Department of Chemical Engineering & Biotechnology, Korea Polytechnic University, 237 Sangidaehak-ro, Siheung-si, Gyeonggi-do, 15073, Korea

    Yong Tae Kim

  3. Nano-bio Application Team, National Nanofab Center, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Korea

    Seok Jae Lee & Kyoung G. Lee

Authors
  1. Yunho Choi
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  2. Yong Tae Kim
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  3. Seok Jae Lee
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  4. Eunjung Lee
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  5. Kyoung G. Lee
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  6. Sung Gap Im
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Correspondence to Kyoung G. Lee or Sung Gap Im.

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Acknowledgments: This work was supported by National Research Foundation of Korea (NRF) grants 2017R1A2B3007806 funded by the Ministry of Science and ICT, Republic of Korea and National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (Grant No. 2015M1A2A2056605, Development of encapsulation technology for stable perovskite solar cells). This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (grant number)(2018R1C1B3001553). This work was supported by Nano Open Innovation Lab Cooperation Project of NNFC.

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Choi, Y., Kim, Y.T., Lee, S.J. et al. Direct Solvent-Free Modification of the Inner Wall of the Microchip for Rapid DNA Extraction with Enhanced Capturing Efficiency. Macromol. Res. 28, 249–256 (2020). https://doi.org/10.1007/s13233-020-8028-x

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  • DOI: https://doi.org/10.1007/s13233-020-8028-x

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