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Phosphate-Functionalized Stabilized F127 Nanoparticles: Introduction of Discrete Surface Charges and Electrophoretic Determination of Aggregation Number

Macromolecular Research volume 27, pages 657–662 (2019)Cite this article

Abstract

Pluronic F127 forms spherical micelle with a defined number of molecules aggregated in aqueous environments. Such self-assembled micelles dissociate into unimers below certain concentration and temperature. We stabilized the micelles by semi-interpenetrating network (sIPN) formation within the hydrophobic core in the presence of a fluorescent dye. Additionally, by varying the mixing ratio of negatively charged and pristine F127s we prepared thermally stable polymeric nanoparticles with discrete surface charges within nearly same sizes. Using the nanomaterials with prescribed number of charges, we demonstrate that the electrophoretic mobility of nanoparticles is solely depending on number of surface charges. Finally, the aggregation number (Nagg) of F127 was further determined by electrophoresis.

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Authors and Affiliations

  1. Department of Chemistry, Marine-Integrated Bionics Research Center, Pukyong National University, Busan, 48513, Korea

    Juyoung Hwang, Mingyeong Kang, Mila Irva Sari & Minseok Kwak

  2. Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, Korea

    Tae Hyeong Lee & Peter C. W. Lee

  3. Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Korea

    Kibeom Kim & Ja-Hyoung Ryu

  4. Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Korea

    Yeol Kyo Choi & Dong June Ahn

  5. School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, 61005, Korea

    Eunji Lee

  6. Department of Physics, Pukyong National University, Busan, 48513, Korea

    Seung Ki Baek

Authors
  1. Juyoung Hwang
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  2. Mingyeong Kang
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  4. Tae Hyeong Lee
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  10. Dong June Ahn
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  11. Peter C. W. Lee
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Corresponding authors

Correspondence to Peter C. W. Lee or Minseok Kwak.

Additional information

Footnote: The image from this article is used as the cover image of the Volume 27, Issue 7

Acknowledgments: This work was supported by Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Republic of Korea (20150220) and by the Korea Polar Research Institute (PAP 2018).

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Hwang, J., Kang, M., Sari, M.I. et al. Phosphate-Functionalized Stabilized F127 Nanoparticles: Introduction of Discrete Surface Charges and Electrophoretic Determination of Aggregation Number. Macromol. Res. 27, 657–662 (2019). https://doi.org/10.1007/s13233-019-7100-x

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  • DOI: https://doi.org/10.1007/s13233-019-7100-x

Keywords

  • self-assembly
  • encapsulation
  • polymer aggregates
  • Pluronic
  • sIPNs