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Bioactive Antibacterial Modification of Orthodontic Microimplants Using Chitosan Biopolymer

Macromolecular Research volume 27, pages 504–510 (2019)Cite this article

Abstract

Titanium alloy (Ti6Al4V)-based microimplants are extensively used as anchorage units in orthodontic treatment; however, they have a high failure rate (10 to 30%). This study aimed to enhance the survivability of these small implants by improving their bioactive and antibacterial properties, via modifying their surface with chitosan. For a stable covalent bond, microimplant surfaces were first functionalized with 3-triethoxysilylpropylamine (APTES) and then grafted either with a succinic acid or polyacrylic acid spacer, followed by attachment with chitosan. Validity tests were conducted on titanium alloy disk (TI) specimens treated with the same chemical process (TI specimens were used to avoid the practical difficulties in testing the small and complex-shaped microimplants). The results confirmed successful APTES functionalization and grafting of succinic- or polyacrylic acid. Anchoring of chitosan was confirmed via X-ray photoelectron spectroscopy (XPS) and confocal laser scanning microscopy (CLSM); furthermore, the anchoring ability of succinic acid, as a spacer, for chitosan was superior to that of polyacrylic acid. Chitosan-modified TI specimens (TI-SA-Ch) showed better biocompatibility with pre-osteoblastic MC3T3-E1 cells, as confirmed from their enhanced adhesion, proliferation, and from cell viability analysis. Biofilm formation of Streptococcus mutans and Streptococcus sobrinus was effectively reduced by 53% and 31%, respectively, on the surface of TI-SA-Ch. These results clearly indicated that chitosan modification might be a promising approach to enhance the bioactive- and antibacterial properties of orthodontic microimplants, and thus, increase their stability in the jaw bone in clinical practice.

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References

  1. R. Kanomi, J. Clin. Orthod, 36, 763 (1997).

    Google Scholar 

  2. Y.-C. Park, S.-Y. Lee, D.-H. Kim, and S.-H. Lee, Am. J. Orthod. Dentofacial Orthop, 690, 123 (2003).

    Google Scholar 

  3. V. A. Barao, M. T. Mathew, W. G. Assuncao, J. C. Yuan, M. A. Wimmer, and C. Sukotjo, J. Dent. Res., 90, 613 (2011).

    Article  CAS  PubMed  Google Scholar 

  4. S. Karimi and A. M. Alfantazi, Mater. Sci. Eng. C: Mater. BiolAppl., 40, 435 (2014).

    Article  CAS  Google Scholar 

  5. L. N. Wang, X. Q. Huang, A. Shinbine, and J. L. Luo, J. Mater. Sci.-Mater. Medicine, 24, 295 (2013).

    Article  CAS  Google Scholar 

  6. J. Pan, D. Thierry, and C. Leygraf, J. Biomed. Mater. Res., 30, 393 (1996).

    Article  CAS  PubMed  Google Scholar 

  7. D. Cadosch, E. Chan, O. P. Gautschi, and L. Filgueira, J. Biomed. Mater. Res. Part A, 91A, 1252 (2009).

    Article  CAS  Google Scholar 

  8. J. Liao, M. Anchun, Z. Zhu, Y. Quan, Int.J. Nanomedicine, 5, 337(2010).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. O. Addison, A. J. Davenport; R. J. Newport, S. Kalra, M. Monir, J.F.W. Mosselmans, D. Proops, and R. A. Martin, J. R. Soc. Interface, 9, 3161 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. M. Uo, K. Asakura, A. Yokoyama, M. Ishikawa, K. Tamura, Y. Totsuka, T. Akasaka, and F. Watari, Dent Mater. J., 26, 268 (2007).

    Article  CAS  PubMed  Google Scholar 

  11. M. Quirynen, M. De Soete, and D. van Steenberghe, Clin. Oral Implants Res., 13, 1 (2002).

    Article  PubMed  Google Scholar 

  12. J. Lindhe and J. Meyle, J. Clin. Periodontal., 35 (8 Suppl), 282 (2008).

    Article  Google Scholar 

  13. I. Hauser-Gerspach, E. M. Kulik, R Weiger, E.-M. Decker, C. Von Ohle, and J. Meyer, Dent Mater. J., 26, 361 (2007).

    Article  CAS  PubMed  Google Scholar 

  14. D. W. Paquette, N. Brodala, and R. C. Williams, Dent Clin. North Am., 50, 361 (2006).

    Article  PubMed  Google Scholar 

  15. J. C. M. Souza, P. Ponthiaux, M. Henriques, R. Oliveira, W. Teughels, J. P. Celis, and L. A. Rocha, J. Dent., 41, 528 (2013).

    Article  CAS  PubMed  Google Scholar 

  16. D. G. Olmedo, G. Nalli, S. Verdu, M. L. Paparella, and R L. Cabrini, J. Periodontal., 84, 78 (2013).

    Article  Google Scholar 

  17. N. U. Zitzmann and T. Berglundh, J. Clin. Periodontal, 35 (8 Suppl), 286 (2008).

    Article  Google Scholar 

  18. Y. Okazaki, E. Gotoh, T. Manabe, and K. Kobayashi, Biomaterials, 25, 5913 (2004).

    Article  CAS  PubMed  Google Scholar 

  19. C. Sedarat, M. F. Harmand, A. Naji, and H. Nowzari, J. Periodontal Res., 36, 269 (2001).

    Article  CAS  PubMed  Google Scholar 

  20. C. Dawes, J.Am. Dent.Assoc., 139 Supp, 18S (2008).

    Google Scholar 

  21. M. S. Jeon, Y. G. Kang, S. S. Mo, K. H. Lee, Y. A. Kook, and S. H. Kim, J. Orthod., 38, 328 (2008).

    Google Scholar 

  22. J. A. Shibli, C. Mangano, S. D’avila, A. Piattelli, G. E. Pecora, F. Mangano, T. Onuma, L. A. Cardoso, D. S. Ferrari, K.C. Aguiar, and G. Iezzi, J. Biomed. Mater. Res. B: Appl. Biomater, 80, 377 (2007).

    Article  CAS  Google Scholar 

  23. A. G. Rodriguez-Hernandez, A. Juarez, E. Engel, and F. J. Gil, J. Mater. Sci. Mater. Medicine, 22, 1913 (2011).

    Article  CAS  Google Scholar 

  24. A. G. Rodriguez-Hernandez, J. A. Munoz-Tabares, M. Godoy-Gallardo, A. Juarez, and F.-J. Gil, Mater. Sci. Eng. C: Mater. Biol. Appl., 33, 714 (2013).

    Article  CAS  Google Scholar 

  25. T. Wadström, J. Invest. Surg., 2, 353 (1989).

    Article  PubMed  Google Scholar 

  26. S. Hwangbo, J. Heo, X. Lin, M. Choi, and J. Hong, Sci. Rep., 6, 19178 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. H. Jeong Y. A. Cho, Y. Cho, E. Kang, H.-W. Ahn, and J. Hong, ACS Biomater. Sci. Eng., 2, 344 (2016).

    Article  CAS  Google Scholar 

  28. L. Peng, L. Chang, X. Liu, J. Lin, H. Liu, B. Han, and S. Wang, ACS Appl. Mater. Interfaces, 9, 17096 (2017).

    Article  CAS  Google Scholar 

  29. S. Park, H.-H. Kim, S. B. Yang, J.-H. Moon, H.-W. Ahn, and J. Hong, ACS Appl. Mater. Interfaces, 10, 17714 (2018).

    Article  CAS  PubMed  Google Scholar 

  30. M. Choi, L. Xiangde, J. Park, D. Choi, J. Heo, M. Chang, C. Lee, and J. Hong, Chem. Eng. J., 309, 463 (2017).

    Article  CAS  Google Scholar 

  31. S. Hwangbo, H. Jeong, J. Heo, X. Lin, Y. Kim, M. Chang, and J. Hong, React. Funct Polym., 102, 27 (2016).

    Article  CAS  Google Scholar 

  32. W. J. Loesche, Microbiol. Rev., 50, 353 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  33. T.-H. Koo, J. Borah, Z-C. Xing, S.-M. Moon, Y. Jeong, and I.-K Kang, Nanoscale Res. Lett., 8, 1 (2013).

    Article  CAS  Google Scholar 

  34. G. A. O’Toole, J. Vis. Expt, JoVE 47, 1 (2011).

    Google Scholar 

  35. S. A. Gilan, Adv. Microbiol., 3, 543 (2013).

    Article  CAS  Google Scholar 

  36. Z. Shi, K. G. Neoh, E. T. Kang, C. Poh, and W. Wang, J. Biomed. Mater. Res. A, 86, 865 (2008).

    Article  CAS  PubMed  Google Scholar 

  37. Z. Wang, X. Zhang, J. Gu, H. Yang, J. Nie, and G. Ma, Carbohydr. Polym., 103, 38 (2014).

    Article  CAS  PubMed  Google Scholar 

  38. H. Marie, A. Barrere, F. Schoenstein, M. H. Chavanne, B. Grosgogeat, and L. Mora, PLoS One, 7, 30 (2012).

    Google Scholar 

  39. P. H. Chua, K. G. Neoh, E. T. Kang, and W. Wang, Biomaterials, 29, 1412 (2008).

    Article  CAS  PubMed  Google Scholar 

  40. J. D. Bumgardner, R Wiser, P. D. Gerard, P. Bergin, B. Chesnutt M. Marini, V. Ramsey, S. H. Elder, and J. A. Gilbert, J. Biomater. Sci. Polym. Ed., 14, 423 (2003).

    Article  CAS  PubMed  Google Scholar 

  41. K. C. Gupta and F. H. Jabrail, Carbohydr. Polym., 66, 43 (2006).

    Article  CAS  Google Scholar 

  42. K. C. Gupta and F. H. Jabrail, Polym. Adv. Technol, 19, 432 (2008).

    Article  CAS  Google Scholar 

  43. M. B. Haddada, J. Blanchard, and S. Kasale, Gold Bull., 46, 235 (2013).

    Google Scholar 

  44. S. M. Kanan, W. T. Y. Tze, and C. P. Tripp, Langmuir, 18, 6623 (2002).

    Article  CAS  Google Scholar 

  45. S. Renvert and I. Polyzois, J. Clin. Periodontal, 42 (Suppl. 16), S172 (2014).

    Google Scholar 

  46. X. Lin, S. Hwangbo, H. Jeong, Y. A. Cho, H.-W. Ahn, and J. Hong, J. Ind. Eng. Chem., 36, 30 (2016).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Orthodontics, School of Dentistry, Kyungpook National University, 2177 Dalgubeoldae-ro, Jung-gu, Daegu, 41940, Korea

    Nguyen Thi Khanh Ly, Kailash Chandra Gupta & Wonjae Yu

  2. Department of Polymer Science and Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu, 41940, Korea

    Hanbyeol Shin, Kailash Chandra Gupta & Inn Kyu Kang

  3. Polymer Research Laboratory, Department of Chemistry, Indian Institute of Technology, Roorkee, Roorkee, 247 667, India

    Kailash Chandra Gupta

Authors
  1. Nguyen Thi Khanh Ly
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  2. Hanbyeol Shin
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  3. Kailash Chandra Gupta
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  4. Inn Kyu Kang
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  5. Wonjae Yu
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Correspondence to Wonjae Yu.

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Ly, N.T.K., Shin, H., Gupta, K.C. et al. Bioactive Antibacterial Modification of Orthodontic Microimplants Using Chitosan Biopolymer. Macromol. Res. 27, 504–510 (2019). https://doi.org/10.1007/s13233-019-7069-5

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  • DOI: https://doi.org/10.1007/s13233-019-7069-5

Keywords

  • titanium alloy
  • chitosan
  • microimplant
  • antibacterial
  • bone cells interaction