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Surface Charge Modification of Polyethyleneimine for Enhanced Renal Clearance and Bioimaging

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Abstract

Polyethyleneimine (PEI) has emerged as a potentially effective carrier for gene delivery. To overcome the cytotoxicity of PEI in vivo, the biodistribution and clearance of PEI conjugated with near-infrared fluorophores (NIRF) were imaged in mice. To confirm the effect of surface charge on enhanced renal clearance, PEI was conjugated with three different types of NIRF: positively charged PEI-NIRF+, negatively charged PEI-NIRF-, and zwitterionic PEI-NIRF± conjugates, which were intravenously injected into mice. The biodistribution and body clearance were monitored under an NIR fluorescence imaging system at 4 h and 24 h postinjection. All organs and tissues were resected to further compare the distribution of each PEI-NIRF conjugate in two mice at 4 h and 24 h. The results showed that the zwitterionic PEI-NIRF± conjugate was most rapidly cleared, primarily through the kidneys of the mice within 24 h postinjection. In conclusion, the biodistribution of zwitterionic PEI-NIRF± was found to be significantly different from that of positively and negatively charged PEI-NIRF conjugates with increased renal clearance. Thus, zwitterionic PEI-NIRF± could be a promising gene delivery carrier that can potentially reduce the side effects of conventional cationic polymers such as cytotoxicity and non-specific binding in vivo.

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Author notes

  1. These authors equally contributed to this work.

Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Gwangju, 61469, Korea

    Sungsu Lee

  2. Department of Premedical Program, School of Medicine, Chosun University, Gwangju, 61452, Korea

    Wonbong Lim

  3. Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, 61469, Korea

    Jin Seok Jung, Danbi Jo, Gayoung Jo & Hoon Hyun

  4. Department of Surgery, Chonnam National University Medical School, Gwangju, 61469, Korea

    Min Ho Park

Authors
  1. Sungsu Lee
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  2. Wonbong Lim
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  3. Jin Seok Jung
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  4. Danbi Jo
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  5. Gayoung Jo
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  6. Min Ho Park
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  7. Hoon Hyun
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Corresponding authors

Correspondence to Min Ho Park or Hoon Hyun.

Additional information

Acknowledgment: This study was supported by research funding from Chosun University awarded in 2017.

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Lee, S., Lim, W., Jung, J.S. et al. Surface Charge Modification of Polyethyleneimine for Enhanced Renal Clearance and Bioimaging. Macromol. Res. 26, 1251–1256 (2018). https://doi.org/10.1007/s13233-019-7020-9

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  • DOI: https://doi.org/10.1007/s13233-019-7020-9

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