Skip to main content
SpringerLink
Log in

Osteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5)

  • Article
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

The purpose of this research was to examine the effect of biphasic hydroxyapatite and b-tricalcium phosphate (HAp-bTCP) immobilized GDF-5 to assess the hypothesis that osteoblast is enhanced and bone formation is promoted when compared to biphasic HAp-bTCP in vitro and in vivo. First, we characterized the release profiles from HAp-bTCP and tested their cell viability, differentiation. After that, we loaded GDF-5 to HAp-bTCP. In the animal study, 4 full thickness critical-size calvarial defects were prepared in a total of 16 male rabbits. The cranial defects were filled with HAP-bTCp, or heparized HAP-bTCP. At 5 and 10 weeks, dissected specimens were processed for histological and histochemical analysis. GDF-5 was considered to be released in a sustained mode from HAp-bTCP. In vitro results provided that the proliferation and differentiation of MG-63 cells to HAp-bTCP-GDF-5 was higher than that of the control group. In addition, in vivo animal studies showed that HAp-bTCP-GDF-5 has a significant improvement to bone formation. HAp-bTCP-GDF-5 is thought to be an excellent biomaterial that delivers osteogenic differentiation factors such as GDF-5, and GDF-5 can be possibly useful as an effective alternative to avail new bone formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. Orsini, T. Traini, A. Scarano, M. Degidi, V. Perrotti, M. Piccirilli, and A. Piattelli, J. Biomed. Mater. Res. Part. B: Appl. Biomater., 74: 448 (2005).

    Article  CAS  Google Scholar 

  2. M. R. Urist, Science, 150: 893 (1965).

    Article  CAS  PubMed  Google Scholar 

  3. J. M. Wozney, V. Rosen, A. J. Celeste, L. M. Mitsock, M. J. Whitters, R. W. Kriz, R. M. Hewick, and E. A. Wang, Science, 242: 1528 (1988).

    Article  CAS  PubMed  Google Scholar 

  4. A. H. Reddi, Nat. Biotechnol., 16: 247 (1998).

    Article  CAS  PubMed  Google Scholar 

  5. F. J. Hughes, W. Turner, G. Belibasakis, and G. Martuscelli, Periodontol. 2000: 41, 48 (2006).

    Article  Google Scholar 

  6. P. J. Boyne, R. E. Marx, M. Nevins, G. Triplett, E. Lazaro, L. C. Lilly, M. Alder, and P. Nummikoski, Int. J. Periodontics Restorative Dent., 17: 11 (1997).

    CAS  PubMed  Google Scholar 

  7. T. H. Howell, J. Fiorellini, A. Jones, M. Alder, P. Nummikoski, M. Lazaro, L. Lilly, and D. Cochran, Int. J. Periodontics Restorative Dent., 17: 124 (1997).

    CAS  PubMed  Google Scholar 

  8. H. Nishitoh, H. Ichijo, M. Kimura, T. Matsumoto, F. Makishima, A. Yamaguchi, H. Yamashita, S. Enomoto, and K. Miyazono, J. Biol. Chem., 271: 21345 (1996).

    Article  CAS  PubMed  Google Scholar 

  9. S. C. Chang, B. Hoang, J. T. Thomas, S. Vukicevic, F. P. Luyten, N. J. Ryba, C. A. Kozak, A. H. Reddi, and M. Moos, Jr., J. Biol. Chem., 269: 28227 (1994).

    CAS  PubMed  Google Scholar 

  10. Y. Morotome, M. Goseki-Sone, I. Ishikawa, and S. Oida, Biochem. Biophys. Res. Commun., 244: 85 (1998).

    Article  CAS  PubMed  Google Scholar 

  11. P. Buxton, C. Edwards, C. W. Archer, and P. Francis-West, J. Bone Joint Surg. Am., 83–A Suppl 1, S23 (2001).

    Google Scholar 

  12. F. H. Shen, Q. Zeng, Q. Lv, L. Choi, G. Balian, X. Li, and C. T. Laurencin, Spine J., 6: 615 (2006).

    Article  PubMed  Google Scholar 

  13. Q. Zeng, X. Li, G. Beck, G. Balian, and F. H. Shen, Bone, 40: 374 (2007).

    Article  CAS  PubMed  Google Scholar 

  14. R. M. Gruber, A. Ludwig, H. A. Merten, M. Achilles, S. Poehling, and H. Schliephake, Clin. Oral Implants Res., 19: 522 (2008).

    Article  PubMed  Google Scholar 

  15. H.-J. Moon, Y.-P. Yun, C.-W. Han, M.-S. Kim, S.-E. Kim, M.-S. Bae, G.-T. Kim, Y.-S. Choi, E.-H. Hwang, J.-W. Lee, J.-M. Lee, C.-H. Lee, D.-S. Kim, and I.-K. Kwon, Biochem. Biophys. Res. Commun., 413: 194 (2011).

    Article  CAS  PubMed  Google Scholar 

  16. S.-E. Kim, S.-H. Song, Y.-P. Yun, B.-J. Choi, I.-K. Kwon, M.-S. Bae, H.-J. Moon, and Y.-D. Kwon, Biomaterials, 32: 366 (2011).

    Article  CAS  PubMed  Google Scholar 

  17. R. Sasisekharan, S. Ernst, and G. Venkataraman, Angiogenesis, 1: 45 (1997).

    Article  CAS  PubMed  Google Scholar 

  18. S. R. Bryant, Int. J. Prosthodont., 11: 470 (1998).

    CAS  PubMed  Google Scholar 

  19. J. A. Porter and J. A. von Fraunhofer, Gen. Dent., 53: 423 (2005).

    PubMed  Google Scholar 

  20. G. Cricchio and S. Lundgren, Clin. Implant Dent. Relat. Res., 5: 161 (2003).

    Article  PubMed  Google Scholar 

  21. R. E. Jung, D. S. Thoma, and C. H. Hammerle, J. Clin. Periodontol., 35: 255 (2008).

    Article  CAS  PubMed  Google Scholar 

  22. R. Gruber, C. Mayer, W. Schulz, W. Graninger, M. Peterlik, G. Watzek, F. P. Luyten, and L. Erlacher, Cytokine, 12: 1630 (2000).

    Article  CAS  PubMed  Google Scholar 

  23. D. A. Puleo, J. Cell. Physiol., 173: 93 (1997).

    Article  CAS  PubMed  Google Scholar 

  24. A. Yamaguchi, T. Ishizuya, N. Kintou, Y. Wada, T. Katagiri, J.M. Wozney, V. Rosen, and S. Yoshiki, Biochem. Biophys. Res. Commun., 220: 366 (1996).

    Article  CAS  PubMed  Google Scholar 

  25. F. P. Koch, C. Weinbach, E. Hustert, B. Al-Nawas, and W. Wagner, Int. J. Periodontics Restorative Dent., 32: 285 (2012).

    PubMed  Google Scholar 

  26. F. P. Koch, J. Becker, H. Terheyden, B. Capsius, W. Wagner, and R. G. M. C. Trial, Clin. Oral Implants Res., 21: 1301 (2010).

    Article  PubMed  Google Scholar 

  27. H. Kuniyasu, H. Yukito, A. Yajima, M. Ochi, H. Kagami, T. Shigino, K. Sakaguchi, and J. Pohl, in 3rd World Congress of Osseointegration, Italy, 2001.

    Google Scholar 

  28. A. Stavropoulos, J. Becker, B. Capsius, Y. Acil, W. Wagner, and H. Terheyden, J. Clin. Periodontol., 38: 966 (2011).

    Article  CAS  PubMed  Google Scholar 

  29. D. P. Magit, T. Maak, N. Trioano, B. Raphael, Q. Hamouria, G. Polzhofer, I. Drespe, T. J. Albert, and J. N. Grauer, Spine (Phila Pa 1976), 31: 2180 (2006).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyung Hee University, Seoul, 02453, Korea

    Joo-Young Ohe, Byung-Joon Choi, Junho Jung & Yong-Dae Kwon

  2. Department of Oral & Maxillofacial Surgery, Graduate School, Kyung Hee University, Seoul, 02453, Korea

    Jeewan Chun, Youngjae Yoon & Yong-Dae Kwon

Authors
  1. Joo-Young Ohe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Byung-Joon Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Junho Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jeewan Chun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Youngjae Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yong-Dae Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yong-Dae Kwon.

Additional information

Acknowledgment: This research was supported by a grant of the Korea Health Technology R&D project through the KHIDI, funded by the Ministry of Health & Welfare, Republic of Korea (HI14C0175).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ohe, JY., Choi, BJ., Jung, J. et al. Osteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5). Macromol. Res. 26, 1011–1018 (2018). https://doi.org/10.1007/s13233-018-6142-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-018-6142-9

Keywords

Search

Navigation