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Development of novel photopolymerizable hyaluronic acid/heparin-based hydrogel scaffolds with a controlled release of growth factors for enhanced bone regeneration

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Abstract

In this study, the effect of novel BMP-2-loaded photo-cured hydrogels on in vitro and in vivo osteogenesis were explored. The well-defined HA-heparin hydrogel was prepared by a photopolymerization of aminoethyl methacrylate (AEMA) grafted hyaluronic acid (HA) and AEMA-grafted heparin. Subsequently, the resulting hydrogels contained BMP-2 at different concentrations. First, a series of BMP-2-loaded hydrogels were characterized to determine their mechanical properties and release profiles. The results from rheology, swelling ratio, and release kinetics suggest that BMP-2 contained HA-g-Hhep gel (B/HA-g-Hhep gel) had sustained release during 4 weeks with minimal initial burst. Furthermore, the released BMP-2 maintained its biological activity. MTT and live/dead assays showed that the B/HA-g-Hhep gel had good biocompatibility to be used as a scaffold for bone regeneration. In vivo studies of B/HA-g-Hhep gel exhibited significantly enhanced osteogenesis, compared with other types of HA-heparin hydrogels. These excellent results indicated that B/HA-g-Hhep gel has a great potential for a controlled release of growth factor, leading to a good bone regeneration.

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Authors and Affiliations

  1. Department of Dental Materials & Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea

    Min Soo Bae, Na Re Ko, Sang Jin Lee, Jung Bok Lee, Dong Nyoung Heo, Wook Byun & Il Keun Kwon

  2. Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea

    Byung-Joon Choi

  3. Department of Biomedical Engineering, College of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea

    Hoon Bong Jeon

  4. Department of Obstetrics & Gynecology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea

    Hee-Jae Jang, Ji-Yoon Ahn & Deok-Sang Hwang

  5. Department of Advanced General Dentistry, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 03722, Korea

    Bock-Young Jung

Authors
  1. Min Soo Bae
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  2. Na Re Ko
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  3. Sang Jin Lee
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Correspondence to Bock-Young Jung or Il Keun Kwon.

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Bae, M.S., Ko, N.R., Lee, S.J. et al. Development of novel photopolymerizable hyaluronic acid/heparin-based hydrogel scaffolds with a controlled release of growth factors for enhanced bone regeneration. Macromol. Res. 24, 829–837 (2016). https://doi.org/10.1007/s13233-016-4112-7

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  • DOI: https://doi.org/10.1007/s13233-016-4112-7

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