Skip to main content
SpringerLink
Log in

In vitro evaluation of lysyl oxidase antibody conjugated nanoparticles

  • Articles
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

In this study, we prepared poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) conjugated to lysyl oxidase-inhibiting antibodies (LOXab) as an anticancer therapy in order to efficiently inhibit the growth of mammary cancer cells. Scanning electron microscopy and dynamic light scattering analysis showed that the antibody-conjugated NPs tested were stable and round, with a mean diameter of 165 nm and surface charge of -15 mV, compared with values of 143 nm and -17 mV for pristine NPs. The pristine NPs at concentrations less than 1,000 ug/ml showed low cytotoxicity and were effectively internalized into cancer cells. Our in vitro studies showed that the LOXab conjugated NPs were two to three times more suppressive of lung, colorectal and breast cancer cells than was the soluble antibody at the same dose. Taken together, our results suggest that NPs conjugated to LOXab constitute a promising nano-therapeutic candidate for inhibiting the growth of mammary cancer cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. Baskar, K. A. Lee, R. Yeo, and K.-W. Yeoh, Int. J. Med. Sci., 9, 193 (2012).

    Article  Google Scholar 

  2. L. Zitvogel, L. Galluzzi, M. J. Smyth, and G. Kroemer, Immunity, 39, 74 (2013).

    Article  CAS  Google Scholar 

  3. Z. Cheng, A. Al Zaki, J. Z. Hui, V. R. Muzykantov, and A. Tsourkas, Science, 338, 903 (2012).

    Article  CAS  Google Scholar 

  4. P. Parhi, C. Mohanty, and S. K. Sahoo, Drug Discov. Today, 17, 1044 (2012).

    Article  CAS  Google Scholar 

  5. Y. M. Kim, S. K. Lim, and M. S. Yoon, Polym. Korea, 39, 967 (2015).

    Article  CAS  Google Scholar 

  6. S.-H. Kim, S.-M. Choi, B.-S. Youn, and H.-S. Kim, Tissue Eng. Regen. Med., 12, 324 (2015).

    Article  CAS  Google Scholar 

  7. L. Brannon-Peppas and J. O. Blanchette, Adv. Drug Deliv. Rev., 64, 206 (2012).

    Article  Google Scholar 

  8. C. Deng, Y. Jiang, R. Cheng, F. Meng, and Z. Zhong, Nano Today, 7, 467 (2012).

    Article  CAS  Google Scholar 

  9. M. Babaei, M. Ardjmand, A. Akbarzadeh, and A. Seyfkordi, Tissue Eng. Regen. Med., 11, 350 (2014).

    Article  CAS  Google Scholar 

  10. J. S. Seo, Y. J. Jeong, Y. J. Lee, Y. G. Kim, T. T. N. Van, L. Li, E. Lee, H. C. Kang, and K. M. Huh, Polym. Korea, 40, 54 (2016).

    Article  CAS  Google Scholar 

  11. N. Kamaly, Z. Xiao, P. M. Valencia, A. F. Radovic-Moreno, and O. C. Farokhzad, Chem. Soc. Rev., 41, 2971 (2012).

    Article  CAS  Google Scholar 

  12. F. Fay and C. J. Scott, Immunotherapy, 3, 381 (2011).

    Article  CAS  Google Scholar 

  13. S. D. Steichen, M. Caldorera-Moore, and N. A. Peppas, Eur. J. Pharm. Sci., 48, 416 (2013).

    Article  CAS  Google Scholar 

  14. Y. M. Kim, S. K. Lim, and M. S. Yoon, Polym. Korea, 39, 967 (2015).

    Article  CAS  Google Scholar 

  15. D. Bernard, Cancer Cell Microenviron., 1, 10 (2014).

    Google Scholar 

  16. L. Wei, X.-R. Song, J.-J. Sun, X.-W. Wang, L. Xie, and L.-Y. Lv, Cancer Biother. Radiopharm., 27, 672 (2012).

    Article  CAS  Google Scholar 

  17. A.-M. Baker, D. Bird, J. C. Welti, M. Gourlaouen, G. Lang, G. I. Murray, A. R. Reynolds, T. R. Cox, and J. T. Erler, Cancer Res., 73, 583 (2013).

    Article  CAS  Google Scholar 

  18. S. Acharya and S. K. Sahoo, Adv. Drug Deliv. Rev., 63, 170 (2011).

    Article  CAS  Google Scholar 

  19. F. Danhier, E. Ansorena, J. M. Silva, R. Coco, A. Le Breton, and V. Preat, J. Control. Release, 161, 505 (2012).

    Article  CAS  Google Scholar 

  20. M. Chen, H. Ouyang, S. Zhou, J. Li, and Y. Ye, Cell. Immunol., 287, 91 (2014).

    Article  CAS  Google Scholar 

  21. R. A. Rosalia, L. J. Cruz, S. van Duikeren, A. T. Tromp, A. L. Silva, W. Jiskoot, T. de Gruijl, C. Lowik, J. Oostendorp, and S. H. van der Burg, Biomaterials, 40, 88 (2015).

    Article  CAS  Google Scholar 

  22. M. Trif, P. E. Florian, A. Roseanu, M. Moisei, O. Craciunescu, C. E. Astete, and C. M. Sabliov, J. Biomed. Mater. Res. A, 103, 3599 (2015).

    Article  CAS  Google Scholar 

  23. T.-G. Iversen, T. Skotland, and K. Sandvig, Nano Today., 6, 176 (2011).

    Article  CAS  Google Scholar 

  24. I. Canton and G. Battaglia, Chem. Soc. Rev., 41, 2718 (2012).

    Article  CAS  Google Scholar 

  25. H. E. Barker, T. R. Cox, and J. T. Erler, Nat. Rev. Cancer, 12, 540 (2012).

    Article  CAS  Google Scholar 

  26. Q. Xiao and G. Ge, Cancer Microenviron., 5, 261 (2012).

    Article  CAS  Google Scholar 

  27. C. C.-L. Wong, H. Zhang, D. M. Gilkes, J. Chen, H. Wei, P. Chaturvedi, M. E. Hubbi, and G. L. Semenza, J. Mol. Med., 90, 803 (2012).

    Article  CAS  Google Scholar 

  28. A. Bondareva, C. M. Downey, F. Ayres, W. Liu, S. K. Boyd, B. Hallgrimsson, and F. R. Jirik, PLoS One, 4, e5620 (2009).

    Article  Google Scholar 

  29. L.-C. Chen, S.-H. Tu, C.-S. Huang, C.-S. Chen, C.-T. Ho, H.-W. Lin, C.-H. Lee, H.-W. Chang, C.-H. Chang, and C.-H. Wu, Breast Cancer Res. Treat., 134, 989 (2012).

    Article  CAS  Google Scholar 

  30. H. M. Rodriguez, M. Vaysberg, A. Mikels, S. McCauley, A. C. Velayo, C. Garcia, and V. Smith, J. Biol. Chem., 285, 20964 (2010).

    Article  CAS  Google Scholar 

  31. M. Kanapathipillai, A. Mammoto, T. Mammoto, J. H. Kang, E. Jiang, K. Ghosh, N. Korin, A. Gibbs, R. Mannix, and D. E. Ingber, Nano Lett., 12, 3213 (2012).

    Article  CAS  Google Scholar 

  32. Q. Liu, X. Shao, J. Chen, Y. Shen, C. Feng, X. Gao, Y. Zhao, J. Li, Q. Zhang, and X. Jiang, Toxicol. Appl. Pharm., 251, 79 (2011).

    Article  CAS  Google Scholar 

  33. H. K. Makadia and S. J. Siegel, Polymers, 3, 1377 (2011).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul, 01812, Korea

    Min Sup Kim, Sang Jun Park, Bon Kang Gu & Chun-Ho Kim

  2. Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, 01812, Korea

    Hyun-Jin Shin & Kee-Ho Lee

  3. Department of Surgery, Korea Institute of Radiological and Medical Sciences, Seoul, 01812, Korea

    Sang Bum Kim

Authors
  1. Min Sup Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sang Jun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bon Kang Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hyun-Jin Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kee-Ho Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sang Bum Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chun-Ho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chun-Ho Kim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, M.S., Park, S.J., Gu, B.K. et al. In vitro evaluation of lysyl oxidase antibody conjugated nanoparticles. Macromol. Res. 24, 748–755 (2016). https://doi.org/10.1007/s13233-016-4092-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-016-4092-7

Keywords

Search

Navigation