Macromolecular Research

, Volume 24, Issue 2, pp 131–142 | Cite as

Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a co-culture system

  • Boram Lee
  • Muhammad Shafiq
  • Youngmee Jung
  • Jong-Chul Park
  • Soo Hyun KimEmail author


Tissue-engineered vascular scaffolds provide a promising solution for the replacement of diseased vascular structures. However, a major challenge lies in enhancing endothelialization, host cell ingrowth, and angiogenesis. In this study, we investigated the feasibility of developing a bio-tubular scaffold from human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVEC) co-cultured on electrospun poly(L-lactide-co-ε-caprolactone) membranes to address these issues. Confluent layers of HDFs stimulated the organization of HUVECs into capillary-like networks in an indirect contact (two-dimensional) co-culture on membranes. Bio-tubular scaffolds fabricated from co-cultured membranes were either grown statically in vitro or implanted subcutaneously in severe combined immunodeficient mice for up to 4 weeks for biocompatibility evaluation and functional performance. In vitro examination of co-cultures on scaffolds showed collagen remodeling and an improvement in biomechanical properties up to day 14. Morphological analysis of in vitro grown bio-tubular scaffolds revealed good attachment and growth of both cell types. After one month, co-cultured scaffolds in vivo showed higher infiltration of host cells and collagen remodeling as compared to the HDF-seeded grafts. After 4 weeks, thin continuous layers of endothelial cells and smooth muscle cells were formed as shown by staining with an antibody specific for CD31and α-actin (α-SMA). On the contrary, HDF-seeded scaffolds remained free of α-SMA-positive cells at all time points, whereas few CD31+ cells appeared after 4 weeks. Thus, co-cultured membranes provide a solution for enhancing endothelialization, tissue regeneration, and growth in bio-tubular scaffolds and may have broader applications in regenerative medicine.


angiogenesis endothelial cells electrospinning vascular scaffolds PLCL 


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Copyright information

© The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht 2016

Authors and Affiliations

  • Boram Lee
    • 1
    • 2
  • Muhammad Shafiq
    • 1
    • 4
  • Youngmee Jung
    • 1
    • 4
  • Jong-Chul Park
    • 2
  • Soo Hyun Kim
    • 1
    • 3
    • 4
    Email author
  1. 1.Center for Biomaterials, Biomedical Research InstituteKorea Institute of Science and TechnologyCheongryang, SeoulKorea
  2. 2.Department of Medical ScienceYonsei UniversitySeoulKorea
  3. 3.KU-KIST Graduate School of Converging Science and TechnologyKorea UniversitySeoulKorea
  4. 4.Korea University of Science and TechnologyDaejeonKorea

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