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Macromolecular Research

, Volume 23, Issue 12, pp 1168–1173 | Cite as

Bone-regenerative activity of parathyroid hormone-releasing nano-hydroxyapatite/poly(L-lactic acid) hybrid scaffolds

  • Ahn Na Koo
  • Joo-Young Ohe
  • Deok-Won Lee
  • Jeewan Chun
  • Hong Jae Lee
  • Yong-Dae KwonEmail author
  • Sang Cheon LeeEmail author
Article

Abstract

We developed a bone-regenerative scaffold based on systematic combination of porous organic-inorganic hybrid scaffolds and recombinant human parathyroid hormone (rhPTH). The hybrid scaffold was fabricated by immobilization of polyphosphate-functionalized nano-hydroxyapatite (PP-n-HAp) on the surface of porous poly(L-lactic acid) (PLLA) scaffolds, which was followed by rhPTH loading on the polyphosphates of n-HAp surfaces. The surface polyphosphate functionalities of PP-n-HAp enabled the stable chemical immobilization of n-HAp on the amine-treated pore surface of the PLGA scaffolds. rhPTH with a positive charge was bound at a high efficiency of 98.1~99.5% onto the anionic polyphosphates of PP-n-HAp immobilized on PLLA surfaces and was sustainably released for up to 50 days. The release rate was manipulated by adjusting the amount of loaded rhPTH, and the release data were moderately fitted to the Higuchi’s diffusion model. Four types of scaffolds were tested in rabbit calvarias models (PLLA only, PP-n-HAp-PLLA, rhPTH (2 µg) loaded PP-n-HAp-PLLA, and rhPTH (10 µg) loaded PP-n-HAp-PLLA). After 5 weeks, rhPTH-loaded PP-n-HAp-PLLA (2 and 10 µg of rhPTH) displayed higher bone growth than the control (PLLA only) group. Nano-HAp and sustained release of rhPTH might be synergistically able to enhance the bone healing in the animal model.

Keywords

parathyroid hormone nano-hydroxyapatite sustained release poly(L-lactic acid) surface immobilization 

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Copyright information

© The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht 2015

Authors and Affiliations

  • Ahn Na Koo
    • 1
  • Joo-Young Ohe
    • 2
  • Deok-Won Lee
    • 2
  • Jeewan Chun
    • 2
  • Hong Jae Lee
    • 1
  • Yong-Dae Kwon
    • 2
    Email author
  • Sang Cheon Lee
    • 1
    Email author
  1. 1.Department of Maxillofacial Biomedical Engineering & Institute of Oral Biology, School of DentistryKyung Hee UniversitySeoulKorea
  2. 2.Department of Oral & Maxillofacial Surgery, School of DentistryKyung Hee UniversitySeoulKorea

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