Skip to main content
SpringerLink
Log in

Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing

  • Articles
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

This study focused on the development of biomedicated electrospun nanofiber mats for preventing wound infections and accelerating wound healing. Polycaprolactone (PCL) nanofibers-loaded curcumin (Cur) and polyethylene glycol (PEG) were generated by an electrospinning technique. The change in surface morphology of the electrospun nanofibers to porous surface after immersion was obtained by field emission scanning electron microscopy (FE-SEM). The biological characteristics of the Cur-loaded PCL-PEG nanofiber mats such as cell viability, cell attachment, anti-inflammatory and antibacterial properties, and in vivo wound healing capability were examined. The blending of PEG with PCL resulted in the formation of pores on the nanofibers after immersion, which supports cell viability and proliferation. The mouse myoblast cell line C2C12 showed about 80% viability on the Cur-loaded PCL-PEG nanofiber mat. SEM images showed that the cells could extremely attach and spread out over the surface of the Cur-loaded PCL-PEG nanofiber mat. The inclusion of 0.5 wt% Cur (with respect to PCL) in both the PCL and PCL-PEG blended nanofiber mats inhibited excessive production of nitric oxide (NO) in RAW264.7 mouse macrophages and exhibited good antibacterial activity against Staphylococcus aureus (S. aureus). In vivo wound healing showed that the treatment using Cur-loaded PCL-PEG nanofiber mat significantly increased the rate of wound closure (99%) on day 10 as compared that using PCL nanofiber mat (59%). These results suggest that the PCL nanofiber matrix containing Cur and PEG can facilitate wound healing with cell proliferation and anti-inflammatory properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. Zahedi, I. Rezaeian, S.-O. Ranaei-Siadat, S.-H. Jafari, and P. Supaphol, Polym. Adv. Technol., 21, 77 (2010).

    Article  CAS  Google Scholar 

  2. D. Queen, H. Orsted, H. Sanada, and G. Sussman, Int. Wound J., 1, 59 (2004).

    Article  Google Scholar 

  3. M. Jannesari, J. Varshosaz, M. Morshed, and M. Zamani, Int. J. Nanomedicine, 6, 993 (2011).

    CAS  Google Scholar 

  4. H. J. Rutten and P. H. Nijhuis, Eur. J. Surg. Suppl., 31 (1997).

    Google Scholar 

  5. H. El-Sherif, M. El-Masry, and A. Kansoh, Macromol. Res., 19, 1157 (2011).

    Article  CAS  Google Scholar 

  6. P. Sikareepaisan, U. Ruktanonchai, and P. Supaphol, Carbohydr. Polym., 83, 1457 (2011).

    Article  CAS  Google Scholar 

  7. M. S. Kim, K. D. Hong, H. W. Shin, S. H. Kim, S. H. Kim, M. S. Lee, W. Y. Jang, G. Khang, and H. B. Lee, Int. J. Biol. Macromol., 36, 54 (2005).

    Article  CAS  Google Scholar 

  8. H. Al-Youssef, M. Amina, S. Hassan, T. Amna, J. Jeong, K.-T. Nam, and H. Kim, Macromol. Res., 21, 589 (2013).

    Article  CAS  Google Scholar 

  9. J. Zeng, X. Xu, X. Chen, Q. Liang, X. Bian, L. Yang, and X. Jing, J. Control. Release, 92, 227 (2003).

    Article  CAS  Google Scholar 

  10. J. Lee, J. Kim, Y. Chang, and S. Park, Macromol. Res., 15, 205 (2007).

    Article  CAS  Google Scholar 

  11. T. Nguyen, C. Ghosh, S.-G. Hwang, L. Tran, and J. Park, J. Mater. Sci., 48, 7125 (2013).

    Article  CAS  Google Scholar 

  12. H. R. Pant, M. P. Neupane, B. Pant, G. Panthi, H. J. Oh, M. H. Lee, and H. Y. Kim, Colloids Surf. B: Biointerfaces, 88, 587 (2011).

    Article  CAS  Google Scholar 

  13. X. Xu, L. Yang, X. Xu, X. Wang, X. Chen, Q. Liang, J. Zeng, and X. Jing, J. Control. Release, 108, 33 (2005).

    Article  CAS  Google Scholar 

  14. M. S. Khil, D. I. Cha, H. Y. Kim, I. S. Kim, and N. Bhattarai, J. Biomed. Mater. Res. B: Appl. Biomater., 67, 675 (2003).

    Article  Google Scholar 

  15. J. Ko, H. Yin, J. An, D. Chung, J.-H. Kim, S. Lee, and D. Pyun, Macromol. Res., 18, 137 (2010).

    Article  CAS  Google Scholar 

  16. H. Au, L. Pham, T. Vu, and J. Park, Macromol. Res., 20, 51 (2012).

    Article  CAS  Google Scholar 

  17. E. Luong-Van, L. Grøndahl, K. N. Chua, K. W. Leong, V. Nurcombe, and S. M. Cool, Biomaterials, 27, 2042 (2006).

    Article  CAS  Google Scholar 

  18. T. K. Dash and V. B. Konkimalla, J. Control. Release, 158, 15 (2012).

    Article  CAS  Google Scholar 

  19. J. G. Merrell, S. W. McLaughlin, L. Tie, C. T. Laurencin, A. F. Chen, and L. S. Nair, Clin. Exp. Pharmacol. Physiol., 36, 1149 (2009).

    Article  CAS  Google Scholar 

  20. S. O. Han, W. K. Son, J. H. Youk, T. S. Lee, and W. H. Park, Mater. Lett., 59, 2998 (2005).

    Article  CAS  Google Scholar 

  21. T. T. Nguyen, C. Ghosh, S. G. Hwang, N. Chanunpanich, and J. S. Park, Int. J. Pharm., 439, 296 (2012).

    Article  CAS  Google Scholar 

  22. Y. Z. Zhang, Y. Feng, Z.-M. Huang, S. Ramakrishna, and C. T. Lim, Nanotechnology, 17, 901 (2006).

    Article  CAS  Google Scholar 

  23. R. L. Thangapazham, A. Sharma, and R. K. Maheshwari, Adv. Exp. Med. Biol., 595, 343 (2007).

    Article  Google Scholar 

  24. K.-Y.L. Yang, Lei-Chwen, T.-Y. Tseng, S.-C. Wang, and T.-H. Tsai, J. Chromatogr. B, 853, 183 (2007).

    Article  CAS  Google Scholar 

  25. J. S. Jurenka, Altern. Med. Rev., 14, 141 (2009).

    Google Scholar 

  26. M. Panchatcharam, S. Miriyala, V. Gayathri, and L. Suguna, Mol. Cell. Biochem., 290, 87 (2006).

    Article  CAS  Google Scholar 

  27. G. S. Sidhu, A. K. Singh, D. Thaloor, K. K. Banaudha, G. K. Patnaik, R. C. Srimal, and R. K. Maheshwari, Wound Repair Regen., 6, 167 (1998).

    Article  CAS  Google Scholar 

  28. D. Gopinath, M. R. Ahmed, K. Gomathi, K. Chitra, P. K. Sehgal, and R. Jayakumar, Biomaterials, 25, 1911 (2004).

    Article  CAS  Google Scholar 

  29. X. Wang, Y. Jiang, Y.-W. Wang, M.-T. Huang, C.-T. Ho, and Q. Huang, Food Chem., 108, 419 (2008).

    Article  CAS  Google Scholar 

  30. M. Gou, K. Men, H. Shi, M. Xiang, J. Zhang, J. Song, J. Long, Y. Wan, F. Luo, X. Zhao, and Z. Qian, Nanoscale, 3, 1558 (2011).

    Article  CAS  Google Scholar 

  31. P. Ratanajiajaroen, A. Watthanaphanit, H. Tamura, S. Tokura, and R. Rujiravanit, Eur. Polym. J., 48, 512 (2012).

    Article  CAS  Google Scholar 

  32. O. Suwantong, P. Opanasopit, U. Ruktanonchai, and P. Supaphol, Polymer, 48, 7546 (2007).

    Article  CAS  Google Scholar 

  33. T. T. T. Nguyen, O. H. Chung, and J. S. Park, Carbohydr. Polym., 86, 1799 (2011).

    Article  CAS  Google Scholar 

  34. C.-M. Hsu and S. Shivkumar, Macromol. Mater. Eng., 289, 334 (2004).

    Article  CAS  Google Scholar 

  35. J. Zeng, L. Yang, Q. Liang, X. Zhang, H. Guan, X. Xu, X. Chen, and X. Jing, J. Control. Release, 105, 43 (2005).

    Article  CAS  Google Scholar 

  36. J. H. Sung, M. R. Hwang, J. O. Kim, J. H. Lee, Y. I. Kim, J. H. Kim, S.W. Chang, S. G. Jin, J. A. Kim, W. S. Lyoo, S. S. Han, S. K. Ku, C. S. Yong, and H. G. Choi, Int. J. Pharm., 392, 232 (2010).

    Article  CAS  Google Scholar 

  37. M. Notoya, H. Nishimura, J. T. Woo, K. Nagai, Y. Ishihara, and H. Hagiwara, Eur. J. Pharmacol., 534, 55 (2006).

    Article  CAS  Google Scholar 

  38. A. Scharstuhl, H. A. Mutsaers, S. W. Pennings, W. A. Szarek, F. G. Russel, and F. A. Wagener, J. Cell. Mol. Med., 13, 712 (2009).

    Article  CAS  Google Scholar 

  39. Y. K. Rao, S. H. Fang, and Y. M. Tzeng, J. Ethnopharmacol., 114, 78 (2007).

    Article  CAS  Google Scholar 

  40. J. E. Dunphy, L. C. Edwards, and K. N. Udupa, Ann. Surg., 144, 304 (1956).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering and Research Center of Chemical Technology, Hankyong National University, Gyeonggi, 456-749, Korea

    Hieu Trung Bui & Jun Seo Park

  2. Department of Physics, Sunchon National University, Chunnam, 540-950, Korea

    Ok Hee Chung

  3. College of Veterinary Medicine University of the Philippines Los Banos, Manila, Philippines

    Joseph Dela Cruz

Authors
  1. Hieu Trung Bui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ok Hee Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joseph Dela Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jun Seo Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jun Seo Park.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bui, H.T., Chung, O.H., Dela Cruz, J. et al. Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing. Macromol. Res. 22, 1288–1296 (2014). https://doi.org/10.1007/s13233-014-2179-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-014-2179-6

Keywords

Search

Navigation