Advertisement

Macromolecular Research

, Volume 22, Issue 12, pp 1288–1296 | Cite as

Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing

  • Hieu Trung Bui
  • Ok Hee Chung
  • Joseph Dela Cruz
  • Jun Seo ParkEmail author
Articles

Abstract

This study focused on the development of biomedicated electrospun nanofiber mats for preventing wound infections and accelerating wound healing. Polycaprolactone (PCL) nanofibers-loaded curcumin (Cur) and polyethylene glycol (PEG) were generated by an electrospinning technique. The change in surface morphology of the electrospun nanofibers to porous surface after immersion was obtained by field emission scanning electron microscopy (FE-SEM). The biological characteristics of the Cur-loaded PCL-PEG nanofiber mats such as cell viability, cell attachment, anti-inflammatory and antibacterial properties, and in vivo wound healing capability were examined. The blending of PEG with PCL resulted in the formation of pores on the nanofibers after immersion, which supports cell viability and proliferation. The mouse myoblast cell line C2C12 showed about 80% viability on the Cur-loaded PCL-PEG nanofiber mat. SEM images showed that the cells could extremely attach and spread out over the surface of the Cur-loaded PCL-PEG nanofiber mat. The inclusion of 0.5 wt% Cur (with respect to PCL) in both the PCL and PCL-PEG blended nanofiber mats inhibited excessive production of nitric oxide (NO) in RAW264.7 mouse macrophages and exhibited good antibacterial activity against Staphylococcus aureus (S. aureus). In vivo wound healing showed that the treatment using Cur-loaded PCL-PEG nanofiber mat significantly increased the rate of wound closure (99%) on day 10 as compared that using PCL nanofiber mat (59%). These results suggest that the PCL nanofiber matrix containing Cur and PEG can facilitate wound healing with cell proliferation and anti-inflammatory properties.

Keywords

polycaprolactone curcumin polyethylene glycol electrospinning wound dressing 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Supplementary material

13233_2014_2179_MOESM1_ESM.pdf (460 kb)
Supplementary material, approximately 460 KB.

References

  1. (1).
    P. Zahedi, I. Rezaeian, S.-O. Ranaei-Siadat, S.-H. Jafari, and P. Supaphol, Polym. Adv. Technol., 21, 77 (2010).CrossRefGoogle Scholar
  2. (2).
    D. Queen, H. Orsted, H. Sanada, and G. Sussman, Int. Wound J., 1, 59 (2004).CrossRefGoogle Scholar
  3. (3).
    M. Jannesari, J. Varshosaz, M. Morshed, and M. Zamani, Int. J. Nanomedicine, 6, 993 (2011).Google Scholar
  4. (4).
    H. J. Rutten and P. H. Nijhuis, Eur. J. Surg. Suppl., 31 (1997).Google Scholar
  5. (5).
    H. El-Sherif, M. El-Masry, and A. Kansoh, Macromol. Res., 19, 1157 (2011).CrossRefGoogle Scholar
  6. (6).
    P. Sikareepaisan, U. Ruktanonchai, and P. Supaphol, Carbohydr. Polym., 83, 1457 (2011).CrossRefGoogle Scholar
  7. (7).
    M. S. Kim, K. D. Hong, H. W. Shin, S. H. Kim, S. H. Kim, M. S. Lee, W. Y. Jang, G. Khang, and H. B. Lee, Int. J. Biol. Macromol., 36, 54 (2005).CrossRefGoogle Scholar
  8. (8).
    H. Al-Youssef, M. Amina, S. Hassan, T. Amna, J. Jeong, K.-T. Nam, and H. Kim, Macromol. Res., 21, 589 (2013).CrossRefGoogle Scholar
  9. (9).
    J. Zeng, X. Xu, X. Chen, Q. Liang, X. Bian, L. Yang, and X. Jing, J. Control. Release, 92, 227 (2003).CrossRefGoogle Scholar
  10. (10).
    J. Lee, J. Kim, Y. Chang, and S. Park, Macromol. Res., 15, 205 (2007).CrossRefGoogle Scholar
  11. (11).
    T. Nguyen, C. Ghosh, S.-G. Hwang, L. Tran, and J. Park, J. Mater. Sci., 48, 7125 (2013).CrossRefGoogle Scholar
  12. (12).
    H. R. Pant, M. P. Neupane, B. Pant, G. Panthi, H. J. Oh, M. H. Lee, and H. Y. Kim, Colloids Surf. B: Biointerfaces, 88, 587 (2011).CrossRefGoogle Scholar
  13. (13).
    X. Xu, L. Yang, X. Xu, X. Wang, X. Chen, Q. Liang, J. Zeng, and X. Jing, J. Control. Release, 108, 33 (2005).CrossRefGoogle Scholar
  14. (14).
    M. S. Khil, D. I. Cha, H. Y. Kim, I. S. Kim, and N. Bhattarai, J. Biomed. Mater. Res. B: Appl. Biomater., 67, 675 (2003).CrossRefGoogle Scholar
  15. (15).
    J. Ko, H. Yin, J. An, D. Chung, J.-H. Kim, S. Lee, and D. Pyun, Macromol. Res., 18, 137 (2010).CrossRefGoogle Scholar
  16. (16).
    H. Au, L. Pham, T. Vu, and J. Park, Macromol. Res., 20, 51 (2012).CrossRefGoogle Scholar
  17. (17).
    E. Luong-Van, L. Grøndahl, K. N. Chua, K. W. Leong, V. Nurcombe, and S. M. Cool, Biomaterials, 27, 2042 (2006).CrossRefGoogle Scholar
  18. (18).
    T. K. Dash and V. B. Konkimalla, J. Control. Release, 158, 15 (2012).CrossRefGoogle Scholar
  19. (19).
    J. G. Merrell, S. W. McLaughlin, L. Tie, C. T. Laurencin, A. F. Chen, and L. S. Nair, Clin. Exp. Pharmacol. Physiol., 36, 1149 (2009).CrossRefGoogle Scholar
  20. (20).
    S. O. Han, W. K. Son, J. H. Youk, T. S. Lee, and W. H. Park, Mater. Lett., 59, 2998 (2005).CrossRefGoogle Scholar
  21. (21).
    T. T. Nguyen, C. Ghosh, S. G. Hwang, N. Chanunpanich, and J. S. Park, Int. J. Pharm., 439, 296 (2012).CrossRefGoogle Scholar
  22. (22).
    Y. Z. Zhang, Y. Feng, Z.-M. Huang, S. Ramakrishna, and C. T. Lim, Nanotechnology, 17, 901 (2006).CrossRefGoogle Scholar
  23. (23).
    R. L. Thangapazham, A. Sharma, and R. K. Maheshwari, Adv. Exp. Med. Biol., 595, 343 (2007).CrossRefGoogle Scholar
  24. (24).
    K.-Y.L. Yang, Lei-Chwen, T.-Y. Tseng, S.-C. Wang, and T.-H. Tsai, J. Chromatogr. B, 853, 183 (2007).CrossRefGoogle Scholar
  25. (25).
    J. S. Jurenka, Altern. Med. Rev., 14, 141 (2009).Google Scholar
  26. (26).
    M. Panchatcharam, S. Miriyala, V. Gayathri, and L. Suguna, Mol. Cell. Biochem., 290, 87 (2006).CrossRefGoogle Scholar
  27. (27).
    G. S. Sidhu, A. K. Singh, D. Thaloor, K. K. Banaudha, G. K. Patnaik, R. C. Srimal, and R. K. Maheshwari, Wound Repair Regen., 6, 167 (1998).CrossRefGoogle Scholar
  28. (28).
    D. Gopinath, M. R. Ahmed, K. Gomathi, K. Chitra, P. K. Sehgal, and R. Jayakumar, Biomaterials, 25, 1911 (2004).CrossRefGoogle Scholar
  29. (29).
    X. Wang, Y. Jiang, Y.-W. Wang, M.-T. Huang, C.-T. Ho, and Q. Huang, Food Chem., 108, 419 (2008).CrossRefGoogle Scholar
  30. (30).
    M. Gou, K. Men, H. Shi, M. Xiang, J. Zhang, J. Song, J. Long, Y. Wan, F. Luo, X. Zhao, and Z. Qian, Nanoscale, 3, 1558 (2011).CrossRefGoogle Scholar
  31. (31).
    P. Ratanajiajaroen, A. Watthanaphanit, H. Tamura, S. Tokura, and R. Rujiravanit, Eur. Polym. J., 48, 512 (2012).CrossRefGoogle Scholar
  32. (32).
    O. Suwantong, P. Opanasopit, U. Ruktanonchai, and P. Supaphol, Polymer, 48, 7546 (2007).CrossRefGoogle Scholar
  33. (33).
    T. T. T. Nguyen, O. H. Chung, and J. S. Park, Carbohydr. Polym., 86, 1799 (2011).CrossRefGoogle Scholar
  34. (34).
    C.-M. Hsu and S. Shivkumar, Macromol. Mater. Eng., 289, 334 (2004).CrossRefGoogle Scholar
  35. (35).
    J. Zeng, L. Yang, Q. Liang, X. Zhang, H. Guan, X. Xu, X. Chen, and X. Jing, J. Control. Release, 105, 43 (2005).CrossRefGoogle Scholar
  36. (36).
    J. H. Sung, M. R. Hwang, J. O. Kim, J. H. Lee, Y. I. Kim, J. H. Kim, S.W. Chang, S. G. Jin, J. A. Kim, W. S. Lyoo, S. S. Han, S. K. Ku, C. S. Yong, and H. G. Choi, Int. J. Pharm., 392, 232 (2010).CrossRefGoogle Scholar
  37. (37).
    M. Notoya, H. Nishimura, J. T. Woo, K. Nagai, Y. Ishihara, and H. Hagiwara, Eur. J. Pharmacol., 534, 55 (2006).CrossRefGoogle Scholar
  38. (38).
    A. Scharstuhl, H. A. Mutsaers, S. W. Pennings, W. A. Szarek, F. G. Russel, and F. A. Wagener, J. Cell. Mol. Med., 13, 712 (2009).CrossRefGoogle Scholar
  39. (39).
    Y. K. Rao, S. H. Fang, and Y. M. Tzeng, J. Ethnopharmacol., 114, 78 (2007).CrossRefGoogle Scholar
  40. (40).
    J. E. Dunphy, L. C. Edwards, and K. N. Udupa, Ann. Surg., 144, 304 (1956).CrossRefGoogle Scholar

Copyright information

© The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht 2014

Authors and Affiliations

  • Hieu Trung Bui
    • 1
  • Ok Hee Chung
    • 2
  • Joseph Dela Cruz
    • 3
  • Jun Seo Park
    • 1
    Email author
  1. 1.Department of Chemical Engineering and Research Center of Chemical TechnologyHankyong National UniversityGyeonggiKorea
  2. 2.Department of PhysicsSunchon National UniversityChunnamKorea
  3. 3.College of Veterinary Medicine University of the Philippines Los BanosManilaPhilippines

Personalised recommendations