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Amino acid-modified bioreducible poly(amidoamine) dendrimers: Synthesis, characterization and In vitro evaluation

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Abstract

Poly(amidoamine) (PAMAM) dendrimers are synthetic polymers commonly used as carriers in gene and drug delivery. PAMAMs have the ability to transfect DNA, but their transfection efficiency is currently insufficient for clinical use. Here, we demonstrate the synthesis and evaluation of cationic dendrimers consisting of a cystamine core PAMAM generation 3 (cPAM G3) and amino acids. Introduction of histidine (His) and arginine (Arg) residues to cPAM G3 resulted in high transfection efficiency and low cytotoxicity. cPAM G3-His-Arg formed stable polyplexes at a weight ratio of 6:1, and the mean polyplex diameter was 99.03±1.68 nm. Nano-sized polyplexes increased in diameter up to 132.93±1.79 nm when treated with a reducing agent, dithiothreitol (DTT). cPAM G3-His-Arg showed much higher transfection efficiency than native cPAM G3 and polyethylenimine (PEI, 25KD). In addition, cPAM G3-His-Arg displayed negligible toxicity, even at high polymer concentrations. Finally, confocal laser microscopy results showed that cPAM G3-His-Arg effectively internalized plasmid DNA into the cells. Therefore, we believe that cPAM G3-His-Arg could be a promising bioreducible vector for non-viral gene delivery with high gene transfection efficiency and low cytotoxicity.

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Authors and Affiliations

  1. Department of Biochemistry, Chungnam National University, Daejeon, 305-764, Korea

    Gwang Sig Yu, Yun Mi Bae, Ji Young Kim & Joon Sig Choi

  2. Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan, 614-735, Korea

    Jin Han

  3. Department of Internal Medicine, Sanggye Paik Hospital, Cardiovascular and Metabolic Disease Center, Inje University, Seoul, 100-032, Korea

    Kyung Soo Ko

  4. Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Korea

    Joon Sig Choi

Authors
  1. Gwang Sig Yu
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  2. Yun Mi Bae
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  3. Ji Young Kim
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  4. Jin Han
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  5. Kyung Soo Ko
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Correspondence to Kyung Soo Ko or Joon Sig Choi.

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The first two authors contributed equally to this work.

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Yu, G.S., Bae, Y.M., Kim, J.Y. et al. Amino acid-modified bioreducible poly(amidoamine) dendrimers: Synthesis, characterization and In vitro evaluation. Macromol. Res. 20, 1156–1162 (2012). https://doi.org/10.1007/s13233-012-0164-5

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  • DOI: https://doi.org/10.1007/s13233-012-0164-5

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