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Synthesis and characterization of chemiluminescent conducting polyluminol via biocatalysis

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Abstract

The enzyme catalyzed polymerization of luminol (5-amino-2, 3-dihydroxy-1, 4-phthalazinedione) in the presence of polystyrenesulphonate (SPS) is introduced. Polymerization was achieved using horseradish peroxidase (HRP) as a biocatalyst in a phosphate buffer at pH 8. A green, water-soluble conducting PLUM/SPS complex was obtained. The physico-chemical properties of the product, including possible photoluminescence of the PLUM/SPS complex, were studied. The PLUM/SPS complex was shown to be a chemiluminescent (CL) material that is highly sensitive towards Fe3+ ions. The quantum yield of the PLUM chemiluminescence was determined to be moderate (0.09 ± 0.01) compared to other chemiluminescent compounds. UV-visible, FTIR, 1H NMR, GPC and conductivity measurements confirmed that the electroactive form of polyluminol (PLUM) was formed and complexed to the SPS.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Shahid Beheshti University, G. C., 1983963113, Tehran, Iran

    Mohammad Reza Nabid, Soheil Salman Taheri, Roya Sedghi & Seyed Jamal Tabatabaei Rezaei

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  1. Mohammad Reza Nabid
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  2. Soheil Salman Taheri
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  3. Roya Sedghi
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  4. Seyed Jamal Tabatabaei Rezaei
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Correspondence to Mohammad Reza Nabid.

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Nabid, M.R., Taheri, S.S., Sedghi, R. et al. Synthesis and characterization of chemiluminescent conducting polyluminol via biocatalysis. Macromol. Res. 19, 280–285 (2011). https://doi.org/10.1007/s13233-011-0302-5

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