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Berry Polyphenols Inhibit Digestive Enzymes: a Source of Potential Health Benefits?

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Food Digestion

Abstract

This paper provides an overview of work that suggests that polyphenol components from berries can inhibit crucial enzymes involved in starch and lipid digestion and potentially influence blood glucose levels and fat digestion. Polyphenols from certain berries have been found to inhibit lipase activity in vitro at low levels. By screening berries with differing polyphenol composition for their inhibitory effectiveness, certain polyphenol classes were implicated as active components. The inhibition was caused at low levels, certainly achievable in the gut after intake of a small amount of berries and approached inhibition achieved by the pharmaceutical lipase inhibitor, orlistat. Polyphenols from berries also have the potential to modulate starch digestion as they inhibit both α-amylase activity and α-glucosidase activity in vitro at low levels. Different berries showed very different levels of effectiveness against the two enzymes and a comparison of their polyphenol composition indicated which components were the most effective inhibitors. For α-amylase, tannins (ellagitannins and proanthocyanidins) were found to be the most effective components but their effectiveness may be modulated by other polyphenols. The active components in the inhibition of α-glucosidase were less obvious and a range of different polyphenol classes may be effective. In both cases, the berry components could act additively with the pharmaceutically used inhibitor, acarbose, and there is potential for berry components to substitute for acarbose or reduce the dose required for effective glycaemic control.

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References

  1. Anon (2002) Joint WHO/FAO expert consultation on diet, nutrition and the prevention of chronic diseases WHO Technical Report Series, No. 916, Geneva, Switzerland

  2. Kahkonen M, Hopia A, Heinonen M (2001) Berry phenolics and their antioxidant activity. J Agric Food Chem 49:4076–4082

    Article  CAS  Google Scholar 

  3. Halliwell B (1996) Antioxidants in human health and disease. Annu Rev Nutr 16:33–50

    Article  CAS  Google Scholar 

  4. Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79:727–747

    CAS  Google Scholar 

  5. Kay CD, Kroon PA, Cassidy A (2009) The bioactivity of dietary anthocyanins is likely to be mediated by their degradation products. Mol Nutr Food Res 53:S92–S101

    Article  Google Scholar 

  6. Williamson G, Clifford MN (2010) Colonic metabolites of berry polyphenols: the missing link to biological activity? Br J Nutr 104:S48–S66

    Article  CAS  Google Scholar 

  7. McDougall GJ, Shpiro F, Dobson P, Smith P, Blake A, Stewart D (2005) Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase. J Agric Food Chem 53:2760–2766

    Article  CAS  Google Scholar 

  8. McDougall GJ, Kulkarni NN, Stewart D (2009) Berry polyphenols inhibit pancreatic lipase activity in vitro. Food Chem 115:193–199

    Article  CAS  Google Scholar 

  9. Grussu D, Stewart D, McDougall GJ (2011) Berry polyphenols inhibit α-amylase in vitro: identifying active components in rowanberry and raspberry. J Agric Food Chem 59:2324–2331

    Article  CAS  Google Scholar 

  10. Whitson J, McDougall GJ, Ross HA, Lund VA, Hamilton CA, Dominiczak AF, Stewart D (2010) Bioactive berry components: potential modulators of health benefits. Funct Plant Sci Biotechnol 4:34–39

    Google Scholar 

  11. Maatta-Riihinen KR, Kamal-Eldin A, Matiila PH, Gonzalez-Paramas AM, Törrönen R (2004) Distribution and contents of phenolic compounds in eighteen Scandinavian berry species. J Agric Food Chem 52:4477–4486

    Article  Google Scholar 

  12. Moreno DA, Ilic N, Poulev A, Brasaemleet D, Fried SK, Raskin I (2003) Inhibitory effects of grape seed extract on lipases. Nutrition 19:876–879

    Article  CAS  Google Scholar 

  13. Sugiyama H, Akazome Y, Shoji T, Yamaguchi A, Yasue M, Kanda T, Ohtake Y (2007) Oligomeric procyanidins in apple polyphenol are main active components for inhibition of pancreatic lipase and triglyceride absorption. J Agric Food Chem 55:4604–4609

    Article  CAS  Google Scholar 

  14. McDougall GJ, Ross HA, Ikeji M, Stewart D (2008) Berry extracts exert different antiproliferative effects against cervical and colon cancer cells grown in vitro. J Agric Food Chem 56:3016–3023

    Article  CAS  Google Scholar 

  15. Kylli P, Nohynek L, Puupponen-Pimiä R, Westerlund-Wikström B, McDougall GJ, Stewart D, Heinonen M (2010) Rowanberry phenolics: compositional analysis and bioactivities. J Agric Food Chem 58:11985–11992

    Article  CAS  Google Scholar 

  16. Pinto MD, de Carvalho JE, Lajolo FM, Genovese MI, Shetty K (2010) Evaluation of anti-proliferative, anti-type 2 diabetes, and anti-hypertension potentials of ellagitannins from strawberries (Fragaria x ananassa Duch.) using in vitro models. J Med Food 13:1027–1035

    Article  Google Scholar 

  17. Desseaux V, Kouliekolo R, Moreau Y, Santimone M, Marchis-Mouren G (2002) Mechanism of porcine pancreatic α-amylase: inhibition of amylase and maltopentaose hydrolysis by various inhibitors. Biologia 57:163–170

    CAS  Google Scholar 

  18. Akkarachiyasit S, Charoenlertkul P, Yibchok-anun S, Adisakwattana S (2010) Inhibitory activities of cyanidin and its glycosides and synergistic effect with acarbose against intestinal α-glucosidase and pancreatic α-amylase. Int J Mol Sci 11:3387–3396

    Article  CAS  Google Scholar 

  19. McDougall GJ, Martinussen I, Stewart D (2008) Towards fruitful metabolomics: high throughput analyses of polyphenol composition in berries using direct infusion mass spectrometry. J Chromatogr B 871:362–369

    Article  CAS  Google Scholar 

  20. Slimestad R, Solheim H (2002) Anthocyanins from black currants (Ribes nigrum L.). J Agric Food Chem 50:3228–3231

    Article  CAS  Google Scholar 

  21. Li YQ, Zhou FC, Gao F, Bian JS, Shan F (2009) Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of alpha-glucosidase. J Agric Food Chem 57:11463–11468

    Article  CAS  Google Scholar 

  22. Karle K, Kraus M, Scheppach W, Ackermann M, Ridder F, Richling E (2006) Studies on apple and blueberry fruits constituents: do the polyphenols reach the colon after ingestion? Mol Nutr Food Res 50:418–423

    Article  Google Scholar 

  23. Gonzanlez-Barrio R, Borges G, Mullen W, Crozier A (2010) Bioavailability of anthocyanins and ellagitannins following consumption of raspberries by healthy humans and subjects with an ileostomy. J Agric Food Chem 58:3933–3939

    Article  Google Scholar 

  24. Hofmann T, Glabasnia A, Schwarz B, Wisman KN, Gangwer KA, Hagerman AE (2006) Protein binding and astringent taste of a polymeric procyanidin, 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose, castalagin and grandinin. J Agric Food Chem 54:9503–9509

    Article  CAS  Google Scholar 

  25. McDougall GJ, Stewart D (2005) The inhibitory effects of berry extracts on digestive enzymes. Biofactors 23:189–195

    Article  CAS  Google Scholar 

  26. McDougall GJ, Kulkarni NN, Stewart D (2008) Current developments on the inhibitory effects of berry polyphenols on digestive enzymes. Biofactors 34:73–80

    Article  Google Scholar 

  27. Pal S, Naissides M, Mamo J (2004) Polyphenolics and fat absorption. Int J Obes 28:324–326

    Article  CAS  Google Scholar 

  28. Vadillo M, Ardévol A, Fernández-Larrea J, Pujadas G, Bladé C, Salvadó MJ, Arola L, Blay M (2006) Moderate red wine consumption partially prevents weight gain in rats fed a hyperlipidic diet. J Nutr Biochem 17:139–142

    Article  Google Scholar 

  29. Wolfram S, Wang Y, Thielecke F (2006) Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res 50:176–187

    Article  CAS  Google Scholar 

  30. Juhel C, Armand M, Pafumi Y, Rosier C, Vandermander J, Lairon D (2000) Green tea extract (AR25®) inhibit lipolysis of triglycerides in gastric and duodenal medium in vitro. J Nutr Biochem 11:45–51

    Article  CAS  Google Scholar 

  31. Hsu C-L, Yen G-C (2007) Phenolic compounds: evidence for inhibitory effects against obesity and their underlying molecular signaling mechanisms. Mol Nutr Food Res 52:53–61

    Article  Google Scholar 

  32. Griffiths DW (1986) The inhibition of digestive enzymes by polyphenolic compounds. Adv Exp Med Biol 199:509–516

    Article  CAS  Google Scholar 

  33. Tsuda T (2008) Regulation of adipocyte function by anthocyanins: possibility of preventing the metabolic syndrome. J Agric Food Chem 56:642–646

    Article  CAS  Google Scholar 

  34. Takikawa M, Inoue S, Horio F, Tsuda T (2010) Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice. J Nutr 140:527–533

    Article  CAS  Google Scholar 

  35. Hogan S, Zhang L, Li JR, Sun S, Canning C, Zhou KQ (2010) Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase. Nutr Metab 7:71–78

    Article  Google Scholar 

  36. Törrönen R, Sarkkinen E, Tapola N, Hautaniemi E, Kilpi K, Niskanen L (2010) Berries modify the postprandial plasma glucose response to sucrose in healthy subjects. Br J Nutr 103:1094–1097

    Google Scholar 

  37. Rosenstock JA, Brown A, Fischer J, Jain A, Littlejohn T, Nadeau D, Sussman T, Taylor A, Krol A, Magner J (1998) Efficacy and safety of acarbose in metformin-treated patients with type 2 diabetes. Diabetes Care 21:2050–2055

    Article  CAS  Google Scholar 

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Acknowledgements

We acknowledge funding from the Scottish Government Rural and Environment Science and Analytical Services Division.

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Correspondence to Gordon J. McDougall.

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Fig. S1

Effect of bovine serum albumin (BSA) on inhibition of amylase by rowanberry PACs. The values are averages of triplicate assays ± standard error. Two repeat experiments are shown. Rowanberry PACs were added at 4.5 μg GAE/ml and BSA at 100 μg/ml. Control assays without PACs but with BSA were run to assess any protective effect of BSA on amylase activity. Two experiments (1 and 2) are shown. Different letters (ad) denote significantly different samples at p < 0.05 (Student’s t test). (DOC 40 kb)

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Boath, A.S., Grussu, D., Stewart, D. et al. Berry Polyphenols Inhibit Digestive Enzymes: a Source of Potential Health Benefits?. Food Dig. 3, 1–7 (2012). https://doi.org/10.1007/s13228-012-0022-0

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  • DOI: https://doi.org/10.1007/s13228-012-0022-0

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