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Lichenicolous fungi of the genus Abrothallus (Dothideomycetes: Abrothallales ordo nov.) are sister to the predominantly aquatic Janhulales

Fungal Diversity volume 64, pages 295–304 (2014)Cite this article

Abstract

This study provides new insights on the phylogenetic position of the lichenicolous fungal genus Abrothallus based on six molecular markers (nuSSU, nuLSU, mtSSU, RPB1, RPB2 and TEF-α). In a broad-scale analysis, we detected high support for inclusion of the genus within Dothideomycetes. A further analysis provided support for Abrothallus as a member of the subclass Pleosporomycetidae as a sister group of Jahnulales, an order of aquatic Dothideomycetes. Given the exclusive characters of this group of apotheciate fungi within the Dothidiomycetes, a new monotypic order Abrothallales is here introduced together with the new family Abrothallaceae. In a multi-locus analysis (based on the six loci indicated above plus ITS) restricted to 12 putative Abrothallus species, two clearly separated clades were observed: one comprising species growing on lichens of the families Parmeliaceae and Ramalinaceae, and the second including species that live on lichens of the order Peltigerales and the family Cladoniaceae.

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Acknowledgments

We thank Tiiu Tõrra, Ede Leppik and Martin Kukwa for collecting the specimens and the curator of NY for granting specimens loan. AS would like to thank Kadri Vaidla, Kadi Jairus, Peeter Laas for performing the lab work. We would like to thank the staff of the microscopy facility of the Museo Nacional de Ciencias Naturales (CSIC, Madrid) for their technical assistance, and Ana Burton for revising the English. SPO and AdR were supported by the grant CTM2012-38222-C02-02 from the Spanish Ministry of Economy and Competitiveness. We thank Maria José Malo (Madrid) for her technical assistance during lab work. The work by AS was supported by a grant awarded by the Estonian Science Foundation no. GP1LM7321, target-financing project SF0180012s09 and the European Regional Development Fund (Centre of Excellence FIBIR).

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Authors and Affiliations

  1. Departamento Biología Ambiental, Museo Nacional de Ciencias Naturales (CSIC), c/Serrano 115 dpdo, 28006, Madrid, Spain

    Sergio Pérez-Ortega & Asunción de los Ríos

  2. Institute of Ecology and Earth Sciences, University of Tartu, 40 Lai str., 51005, Tartu, Estonia

    Ave Suija

  3. Facultad de Farmacia, Departamento Biología Vegetal II, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain

    Ana Crespo

Authors
  1. Sergio Pérez-Ortega
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  2. Ave Suija
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  3. Ana Crespo
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  4. Asunción de los Ríos
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Correspondence to Sergio Pérez-Ortega.

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Fig. S1

Six-locus phylogeny (50 % majority rule consensus tree) depicting phylogenetic relationships among classes of Ascomycota including Abrothallus species. Branches in bold indicate PP ≥ 95 % and ML bootstrap values ≥ 75 %. (JPEG 89 kb)

High resolution image (TIFF 4354 kb)

Table S1

Accession numbers corresponding to taxa from different classes of Ascomycota used in the phylogenetic analyses. (DOCX 23 kb)

Table S2

Accession numbers corresponding to taxa from Dothideomycetes and Arthoniomycetes used in the phylogenetic analyses. (DOC 217 kb)

Table S3

Nucleotide substitution models used for each partition in the three different analyses run in this study. (DOCX 10.7 KB)

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Pérez-Ortega, S., Suija, A., Crespo, A. et al. Lichenicolous fungi of the genus Abrothallus (Dothideomycetes: Abrothallales ordo nov.) are sister to the predominantly aquatic Janhulales. Fungal Diversity 64, 295–304 (2014). https://doi.org/10.1007/s13225-013-0269-y

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Keywords

  • Lichenicolous
  • Multi-locus analysis
  • Taxonomy
  • Pleosporomycetidae
  • Jahnulales