Fungal Diversity

, Volume 64, Issue 1, pp 205–231 | Cite as

Molecular phylogeny of the genus Sticta (lichenized Ascomycota: Lobariaceae) in Colombia

  • Bibiana Moncada
  • Robert Lücking
  • Alejandra Suárez


We present a molecular phylogenetic study of the lichen genus Sticta focusing on Colombia, using the ITS fungal barcoding gene for a total of 370 ingroup OTUs, with 322 newly generated sequences. The topology resulting from a maximum likelihood approach does not support current species concepts in Sticta, which use a morphological concept, but in contrast shows that similar morphodemes evolved multiple times independently within the genus. As a consequence, currently applied names such as S. fuliginosa and S. weigelii comprise numerous (up to more than 20) unrelated species-level lineages, which can be distinguished also phenotypically using previously unrecognized characters such as lobe configuration, lobe surface structure, tomentum type, and anatomy of the basal membrane of the cyphellae. We conclude that the genus Sticta contains about four to five times the number of species currently recognized. In Colombia alone, approximately 150 species of Sticta are present.


Lichen-forming fungi Morphological species concept Parallel evolution 


  1. Aptroot A (2008) Sticta alpinotropica, a new saxicolous lichen species from the alpine zone of Mt. Wilhelm, Papua New Guinea. Lichenologist 40:419–422Google Scholar
  2. Aptroot A, Sparrius LB, Lai MJ (2002) New Taiwan macrolichens. Mycotaxon 84:281–292Google Scholar
  3. Armaleo D, Clerc P (1991) Lichen chimeras: DNA analysis suggests that one fungus forms two morphodemes. Exp Mycol 15:1–10CrossRefGoogle Scholar
  4. Ashlock PD (1984) Monophyly: its meaning and importance. In: Duncan T, Stuessy TF (eds) Cladistics: perspectives on the reconstruction of evolutionary history. Columbia University Press, New York, pp 39–46Google Scholar
  5. Bartók K (1994) The family Stictaceae in Romania. Contrib Bot Cluj-Napoca 1993–1994:47–57Google Scholar
  6. Berger S, Stamatakis A, Lücking R (2011) Morphology-based phylogenetic binning of the lichen genera Graphis and Allographa (Ascomycota: Graphidaceae) using molecular site weight calibration. Taxon 60:1450–1457Google Scholar
  7. Blanco O, Crespo A, Divakar PK, Esslinger TL, Hawksworth DL, Lumbsch HT (2004a) Melanelixia and Melanohalea, two new genera segregated from Melanelia (Parmeliaceae) based on molecular and morphological data. Mycol Res 108:873–884PubMedCrossRefGoogle Scholar
  8. Blanco O, Crespo A, Elix JA, Hawksworth DL, Lumbsch HT (2004b) A molecular phylogeny and a new classification of parmelioid lichens containing Xanthoparmelia-type lichenan (Ascomycota: Lecanorales). Taxon 53:959–975CrossRefGoogle Scholar
  9. Brodo IM, Sharnoff SD, Sharnoff S (2001) Lichens of North America. Yale University Press, New HavenGoogle Scholar
  10. Büdel B, Meyer A, Salazar N, Zellner H, Zotz G, Lange OL (2000) Macrolichens of montane rain forests in Panama, Province Chiriqui. Lichenologist 32:539–551CrossRefGoogle Scholar
  11. Burgaz AR, Martínez I (2003) Flora Liquenológica Ibérica. Peltigerales: Lobariaceae, Nephromataceae, Peltigeraceae. Sociedad Española de Liquenología (SEL), MurciaGoogle Scholar
  12. Chen J, Wang Z, Wang L (1994) The lichen genus Sticta from Yunnan of China. Acta Mycol Sinica 13:29–33Google Scholar
  13. Crawford DJ (2010) Progenitor-derivative species pairs and plant speciation. Taxon 59:1413–1423Google Scholar
  14. Culberson WL (1969) The use of chemistry in the systematics of the lichens. Taxon 18:152–166CrossRefGoogle Scholar
  15. Degnan JH, Rosenberg NA (2009) Gene tree discordance, phylogenetic inference and the multispecies coalescent. Trends Ecol Evol 24:332–340PubMedCrossRefGoogle Scholar
  16. Delise D (1825) Histoire de Lichens. Genre Sticta. Mém Soc Linn Normandie [Calvados] 2:1–167Google Scholar
  17. Duclaux G (1997) Likenoj de Okcidenta Europa. Les Stictacees: les genres Lobaria et Sticta d’après G. Clauzade & C. Roux. Bull d’Inform Assoc Fr Lichénol 22:7–11Google Scholar
  18. Elix JA, Johnston J, Parker JL (1988) A computer program for the rapid identification of lichen substances. Mycotaxon 31:89–99Google Scholar
  19. Farkas EE (2003) Contributions to the lichen flora of East Africa Pseudocyphellaria and Sticta. Acta Acad Paed Agriensis Sect Biol 24:235–255Google Scholar
  20. Funk DJ, Omland KE (2003) Species -level paraphyly and polyphyly. Ann Rev Ecol Evol Syst 34:397–423CrossRefGoogle Scholar
  21. Galloway DJ (1994) Studies on the lichen genus Sticta (Schreber) Ach.: I. Southern South American species. Lichenologist 26:223–282Google Scholar
  22. Galloway DJ (1997) Studies on the lichen genus Sticta (Schreber) Ach. IV. New Zealand species. Lichenologist 29:105–168Google Scholar
  23. Galloway DJ (1998a) Studies on the lichen genus Sticta (Schreber) Ach.: V. Australian species. Trop Bryol 15:117–160Google Scholar
  24. Galloway DJ (1998b) Edvard Vainio and the family Lobariaceae, with special reference to the taxonomic history of Sticta. In: Marcelli MP, Ahti T (eds) Recollecting Edvard August Vainio. CETESB, São Paulo, pp 61–84Google Scholar
  25. Galloway DJ (2001) Sticta. In: McCarthy PM (ed) Flora of Australia. Volume 58A, Lichens 3:78–97. ABRS, CSIRO AustraliaGoogle Scholar
  26. Galloway DJ (2006) Notes on the holotype of Sticta damaecornis f. weigelii Ach. (= Sticta weigelii). Lichenologist 38:89–92CrossRefGoogle Scholar
  27. Galloway DJ (2007) Flora of New Zealand. Lichens. Revised second edition including lichen-forming and lichenicolous fungi. Manaaki Whenua Press, LincolnGoogle Scholar
  28. Galloway DJ, Thomas MA (2004) Sticta. In: Nash TH III, Ryan BD, Diederich P, Gries C, Bungartz F (eds) Lichen Flora of the Greater Sonoran Desert Region 2:513–524. Lichens Unlimited, Arizona State University, TempeGoogle Scholar
  29. Galloway DJ, Stenroos S, Ferraro LI (1995) Flora Criptogámica de Tierra del Fuego. Lichenes Peltigerales: Lobariaceae y Stictaceae, vol 6. Consejo Nacional de Investigaciones Cientificas y Técnicas de la República Argentina, Buenos Aires, Fasc. 6Google Scholar
  30. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rust. Mol Ecol 2:113–118PubMedCrossRefGoogle Scholar
  31. Goward T, McCune B, Meidinger D (1994) The Lichens of British Columbia. Illustrated Keys. Part 1 – Foliose and Squamulose Species. Special Report Series. Research Program, vol 8. B.C. Ministry of Forests, VictoriaGoogle Scholar
  32. Gregory-Wodzicki K (2000) Uplift history of the Central and Northern Andes: a review. Bull Geol Soc Am 112:1091–1105CrossRefGoogle Scholar
  33. Gulyas G, Sramko G, Molnar A, Rudnoy S, Illyes Z, Balazs T, Zoltan B (2005) Nuclear ribosomal DNA ITS paralogs as evidence of recent hybridization in genus Ophrys (Orchidaceae). Acta Biol Crac Ser Bot 47:61–67Google Scholar
  34. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
  35. Harris RC (1984) Sticta, an “easy” genus becomes more difficult. Evansia 1:7–8Google Scholar
  36. Hawksworth DL (1976) Lichen chemotaxonomy. In: Brown DH, Hawksworth DL, Bailey RH (eds) Lichenology: progress and problems. Academic, London, pp 139–184Google Scholar
  37. Hebert PD, Cywinska A, Ball SL, de Waard JR (2003) Biological identifications through DNA barcodes. Proc Roy Soc Lond Ser B Biol Sci 270:313–321CrossRefGoogle Scholar
  38. Heiðmarsson S, Mattsson JE, Moberg R, Nordin A, Santesson R, Tibell L (1997) Classification of lichen photomorphs. Taxon 46:519–520CrossRefGoogle Scholar
  39. Hillis D, Bull J (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic. Syst Biol 42:182–192Google Scholar
  40. Högnabba F, Stenroos S, Thell A (2009) Phylogenetic relationship and evolution of photobiont associations in the Lobariaceae (Peltigerales, Lecanoromycetes, Ascomycota). Biblioth Lichenol 100:157–187Google Scholar
  41. Hoorn C, Wesselingh FP, Ter Steege T, Bermudez MA, Mora A, Sevink J, Sanmartín I, Sanchez-Meseguer A, Anderson CL, Figueiredo F, Jaramillo C, Riff D, Negri FR, Hooghiemstra H, Lundberg J, Stadler T, Sarkinen T, Antonelli A (2010) Amazonia through time: Andean uplift, climate change, landscape evolution and biodiversity. Science 330:927–931PubMedCrossRefGoogle Scholar
  42. Hörandl E (2006) Paraphyletic versus monophyletic taxa – evolutionary versus cladistic classifications. Taxon 55:564–570CrossRefGoogle Scholar
  43. Hörandl E, Stuessy TF (2010) Paraphyletic groups as natural units of biological classification. Taxon 59:1641–1653Google Scholar
  44. Hughes KW, Petersen RH (2001) Apparent recombination or gene conversion in the ribosomal ITS region of a Flammulina (Fungi, Agaricales) hybrid. Mol Biol Evol 18:94–96PubMedCrossRefGoogle Scholar
  45. Jørgensen PM, Tønsberg T (2007) Lobariaceae. In: Nordic Lichen Flora, volume 3, Cyanolichens. Nordic Lichen Society, Uddevalla, pp 77–86Google Scholar
  46. Joshi M, Awasthi DD (1982) The lichen family Stictaceae in India and Nepal. Biol Mem 7:165–190Google Scholar
  47. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucl Acids Res 30:3059–3066PubMedCrossRefGoogle Scholar
  48. Katoh K, Asimenos G, Toh H (2009) Multiple alignment of DNA sequences with MAFFT. Meth Mol Biol 537:39–64CrossRefGoogle Scholar
  49. Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008) Dictionary of the fungi, 10th edn. CAB International, WallingfordGoogle Scholar
  50. Ko KS, Jung HS (2002) Three nonorthologous ITS1 types are present in a polypore fungus Trichaptum abietinum. Mol Phylogenet Evol 23:112–122PubMedCrossRefGoogle Scholar
  51. Laundon JR (1995) On the classification of lichen photomorphs. Taxon 44:387–389CrossRefGoogle Scholar
  52. Lücking R (1996) Taxonomic studies in foliicolous species of the genus Porina. I. The Porina rufula aggregate. Bot Acta 109:248–260Google Scholar
  53. Lücking R (2008) Foliicolous lichenized fungi. Fl Neotrop Monogr 103:1–866Google Scholar
  54. Lücking R (2012) One species is many: unrecognized levels of diversification of tropical macrolichens in neotropical paramos. In: ATBC 2012: 49th Annual Meeting Bonito-MS, Brasil. Abstracts: S03.OC.01Google Scholar
  55. Lücking R, Kalb K, Essene A (2012) The power of ITS: using megaphylogenies of barcoding genes to reveal inconsistencies in taxonomic identifications of genbank submissions. The 7th IAL Symposium “Lichens: From Genome to Ecosystems in a Changing World”, January 2012, Bangkok (Thailand). Book of Abstracts: 3B-1-O2Google Scholar
  56. Lumbsch HT (2002) Analysis of phenolic products in lichens for identification and taxonomy. In: Kranner I, Beckett RP, Varma AK (eds) Protocols in lichenology. Culturing, biochemistry, ecophysiology and use in biomonitoring. Springer, Berlin, pp 281–295Google Scholar
  57. Lumbsch HT, Parnmen S, Rangsiruji A, Elix JA (2010) Phenotypic disparity and adaptive radiation in the genus Cladia (Lecanorales, Ascomycota). Austral Syst Bot 23:239–247CrossRefGoogle Scholar
  58. Lumbsch HT, Ahti T, Altermann S, Amo De Paz G, Aptroot A, Arup U, Bárcenas Peña A, Bawingan PA, Benatti MN, Betancourt L, Björk CR, Boonpragob K, Brand M, Bungartz F, Cáceres MES, Candan M, Chaves JL, Clerc P, Common R, Coppins BJ, Crespo A, Dal-Forno M, Divakar PK, Duya MV, Elix JA, Elvebakk A, Fankhauser JD, Farkas E, Itatí Ferraro L, Fischer E, Galloway DJ, Gaya E, Giralt M, Goward T, Grube M, Hafellner J, Hernández JE, Herrera Campos MA, Kalb K, Kärnefelt I, Kantvilas G, Killmann D, Kirika P, Knudsen K, Komposch H, Kondratyuk S, Lawrey JD, Mangold A, Marcelli MP, McCune B, Messuti MI, Michlig A, Miranda González R, Moncada B, Naikatini A, Nelsen MP, Øvstedal DO, Palice Z, Papong K, Parnmen S, Pérez-Ortega S, Printzen C, Rico VJ, Rivas Plata E, Robayo J, Rosabal D, Ruprecht U, Salazar Allen N, Sancho L, Santos De Jesus L, Santos Vieira T, Schultz M, Seaward MRD, Sérusiaux E, Schmitt I, Sipman HJM, Sohrabi M, Søchting U, Søgaard MZ, Sparrius LB, Spielmann A, Spribille T, Sutjaritturakan J, Thammathaworn A, Thell A, Thor G, Thüs H, Timdal E, Truong C, Türk R, Umaña Tenorio L, Upreti DK, Van den Boom P, Vivas Rebuelta M, Wedin M, Will-Wolf S, Wirth V, Wirtz N, Yahr R, Yeshitela K, Ziemmeck F, Wheeler T, Lücking R (2011) One hundred new species of lichenized fungi: a signature of undiscovered global diversity. Phytotaxa 18:1–127Google Scholar
  59. Makryi TV (2008) Lichens of the genus Sticta (Lobariaceae) in Russia. Bot Z 93:304–316Google Scholar
  60. McDonald T, Miadlikowska J, Lutzoni F (2003) The lichen genus Sticta in the Great Smoky Mountains: a phylogenetic study of morphologial, chemical, and molecular data. Bryologist 106:61–79CrossRefGoogle Scholar
  61. Meade B, Conrad C (2008) Andean growth and the deceleration of South American subduction: time evolution of a coupled orogen-subduction system. Science 321:1054–1058CrossRefGoogle Scholar
  62. Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE): 1–8. New OrleansGoogle Scholar
  63. Moncada B, Lücking R (2012) Ten new species of Sticta and counting: Colombia as a hot spot for unrecognized diversification in a “conspicuous” macrolichen genus. Phytotaxa 74:1–29Google Scholar
  64. Moncada B, Lücking R, Betancourt L (2013a) Phylogeny of the Lobariaceae (lichenized Ascomycota: Peltigerales), with a reappraisal of the genus Lobariella. Lichenologist 45:203–263Google Scholar
  65. Moncada B, Ardila I, Simijaca D (2013b) Dos nuevas especies crípticas del género Sticta (Ascomycota liquenizados: Lobariaceae) de los Andes Colombianos. Acta Biol Colomb (submitted)Google Scholar
  66. Moncada B, Betancourt-Macuase L, Lücking R (2013c) Nuevas especies del género Sticta (Ascomycota liquenizados: Lobariaceae) del morfotipo fuliginosa sensu lato de Colombia. Rev Biol Trop (submitted)Google Scholar
  67. Moncada B, Coca LF, Lücking R (2013d) Neotropical members of Sticta (lichenized Ascomycota: Lobariaceae) forming photosymbiodemes, with the description of seven new species. Bryologist (submitted)Google Scholar
  68. Moncada B, Coca LF, Lücking R (2013e) Cinco nuevas especies de Sticta (Ascomycota liquenizados: Lobariaceae) con superficie tomentosa de Colombia. Cryptogamie Mycol (submitted)Google Scholar
  69. Moncada B, Lücking R, Coca LF (2013f) Six new apotheciate species of Sticta (lichenized Ascomycota: Lobariaceae) from the Colombian Andes. Lichenologist (submitted)Google Scholar
  70. Moncada B, Lücking R, Suárez A (2013g) Nuevas especies del género Sticta (Ascomycota liquenizados: Lobariaceae) del morfotipo limbata sensu lato de Colombia. Caldasia (submitted)Google Scholar
  71. Moncada B, Suárez A, Lücking R (2013h) Nuevas especies del género Sticta (Ascomycota liquenizados: Lobariaceae) de los morfotipos weigelii y sylvativa sensu lato de Colombia. Caldasia (submitted)Google Scholar
  72. Moncada B, Aguirre-CJ, Lücking R (2013i) Ecogeografía del género Sticta (Ascomycota liquenizados: Lobariaceae) en Colombia. Revista de Biología Tropical (in press)Google Scholar
  73. Mugambi GK, Huhndorf SM (2009) Parallel evolution of hysterothecial ascomata in ascolocularous fungi (Ascomycota, Fungi). Syst Biodivers 7:453–464CrossRefGoogle Scholar
  74. Nylander W (1859) Lichenes Exotici, Lichenes in regionibus exoticis quibusdam vigentes exponit synopticis enumerationibus. Ann Sci Nat Bot 11:205–264Google Scholar
  75. Nylander W (1861) Additamentum ad Lichenographiam Andium Boliviensium. Ann Sci Nat Bot 15:365–382Google Scholar
  76. Nylander W (1865) Enumeration synoptica Sticteorum. Flora 48:296–299Google Scholar
  77. Nylander W (1868) Conspectus synopticus Sticteorum. Bull Soc Linn Norm 2:498–505Google Scholar
  78. Orange A, James PW, White FJ (2001) Microchemical methods for the Identification of Lichens. British Lichen Society, LondonGoogle Scholar
  79. Øvstedal DG, Gremmen NJM (2010) New lichen species from Tristan da Cunha and Gough Island. Folia Cryptog Est 47:43–49Google Scholar
  80. Parnmen S, Rangsiruji A, Mongulsuk P, Boonpragob K, Elix JA, Lumbsch HT (2010) Morphological disparity in Cladoniaceae: the foliose genus Heterodea evolved from fruticose Cladia species (Lecanorales, Ascomycota). Taxon 59:841–849Google Scholar
  81. Parnmen S, Lücking R, Lumbsch HT (2012) Phylogenetic classification at generic level in the absence of distinct phylogenetic patterns of phenotypical variation: a case study in Graphidaceae (Ascomycota), PlosOne (submitted)Google Scholar
  82. Penn O, Privman E, Landan G, Graur D, Pupko T (2010a) An alignment confidence score capturing robustness to guide-tree uncertainty. Mol Biol Evol 27:1759–1767PubMedCrossRefGoogle Scholar
  83. Penn O, Privman E, Ashkenazy H, Landan G, Graur D, Pupko T (2010b) GUIDANCE: a web server for assessing alignment confidence scores. Nucl Acids Res 38:W23–W28PubMedCrossRefGoogle Scholar
  84. Renner B, Galloway DJ (1982) Phycosymbiodemes in Pseudoocyphellaria in New Zealand. Mycotaxon 16:197–231Google Scholar
  85. Rivas Plata E, Lumbsch HT (2011) Parallel evolution and phenotypic disparity in lichenized fungi: a case study in the lichen-forming fungal family Graphidaceae (Ascomycota: Lecanoromycetes: Ostropales). Mol Phyl Evol 61:45–63CrossRefGoogle Scholar
  86. Rivas Plata E, Lücking R, Lumbsch HT (2012a) A new classification for the family Graphidaceae (Ascomycota: Lecanoromycetes: Ostropales). Fung Div 52:107–121CrossRefGoogle Scholar
  87. Rivas Plata E, Mason-Gamer R, Ashley M, Lücking R, Lumbsch HT (2012b) Molecular phylogeny and systematics of the Ocellularia-clade (Ascomycota: Ostropales: Graphidaceae). Taxon 61:1161–1179Google Scholar
  88. Rivas Plata E, Parnmen S, Staiger B, Mangold A, Frisch A, Weerakoon G, Hernández M, JE, Cáceres MES, Kalb K, Sipman HJM, Common RS, Nelsen MP, Lücking R, Lumbsch HT (2013) A molecular phylogeny of Graphidaceae (Ascomycota: Lecanoromycetes: Ostropales) including 428 species. MycoKeys (in press)Google Scholar
  89. Rosenberg NA (2003) The shapes of neutral gene genealogies in two species: probabilities of monophyly, paraphyly, and polyphyly in a coalescent model. Evolution 57:1465–1477PubMedGoogle Scholar
  90. Roumeguère C (1868) Cryptogamie illustrée, ou Histoire des familles naturelles des plantes acotylédones d’Europe coordonnée suivant les dernieres classifications et complétée par les recherches scientifiques les plus récentes. Famille des Lichens, contenant 927 figures, représentant, pour chaque genre, la plante de grandeur naturelle et l’anatomie de ses différents organes de végétation et de reproduction, dessinés au microscope composé. Paris, ToulouseGoogle Scholar
  91. Sanders WB (2001) Composite lichen thalli of Sticta sp. from Brazil, with morphologically similar lobes containing either a chlorobiont or a cyanobiont layer. Symbiosis 31:47–55Google Scholar
  92. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci U S A 109:6241–6246PubMedCentralPubMedCrossRefGoogle Scholar
  93. Sheard JW (1978) The taxonomy of the Ramalina siliquosa species aggregate (lichenized Ascomycetes). Can J Bot 56:916–938CrossRefGoogle Scholar
  94. Singh KP, Sinha GP (2010) Indian lichens: an annotated checklist. Botanical Survey of India. Ministry of Environment and Forests, Salt Lake CityGoogle Scholar
  95. Smith CW, Aptroot A, Coppins BJ, Fletcher A, Gilbert OL, James PW, Wolseley PA (eds) (2009) The Lichens of Great Britain and Ireland. British Lichen Society, LondonGoogle Scholar
  96. Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. Freeman, San FranciscoGoogle Scholar
  97. Soltis P, Soltis D (2003) Applying the bootstrap in phylogeny reconstruction. Stat Sci 18:256–267CrossRefGoogle Scholar
  98. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690PubMedCrossRefGoogle Scholar
  99. Stamatakis A, Hoover P, Rougemont J (2008) A fast bootstrapping algorithm for the RAxML web-Servers. Syst Biol 57:758–771PubMedCrossRefGoogle Scholar
  100. Stocker-Wörgötter E (2002) Resynthesis of photosymbiodemes. In: Kranner I, Beckett RP, Varma AK (eds) Protocols in lichenology. Culturing, biochemistry, ecophysiology and use in biomonitoring. Springer, Berlin, pp 47–60Google Scholar
  101. Suárez A, Lücking R (2013) Sticta viviana (lichenized Ascomycota: Peltigerales: Lobariaceae), a new species from Colombian paramos. Lichenologist 45:153–157CrossRefGoogle Scholar
  102. Swinscow TDV, Krog H (1988) Macrolichens of East Africa. British Museum (Natural History), LondonGoogle Scholar
  103. Syring J, Farrell K, Businsky R, Cronn R, Liston A (2007) Widespread genealogical nonmonophyly in species of Pinus subg. Strobus. Syst Biol 56:163–181PubMedCrossRefGoogle Scholar
  104. Takahashi K, Deguchi H (2004) Taxonomic revision of the genus Sticta in Japan. J Plant Res Suppl 117:41Google Scholar
  105. Takahashi K, Li-Song W, Tsubota H, Deguchi H (2006) Photosymbiodemes Sticta wrightii and Dendriscocaulon sp. (lichenized Ascomycota) from Yunnan, China. J Hattori Bot Lab 100:783–796Google Scholar
  106. Tehler A, Irestedt M (2007) Parallel evolution of lichen growth forms in the family Roccellaceae (Arthoniales, Ascomycota). Cladistics 23:432–454CrossRefGoogle Scholar
  107. Tønsberg T, Goward T (2001) Sticta oroborealis sp. nov. and other Pacific North American lichens forming dendriscocauloid cyanotypes. Bryologist 104:12–23CrossRefGoogle Scholar
  108. Tschermak-Woess E (1995) The taxonomic position of the green phycobiont of Sticta canariensis (Ach.) Bory ex Delise and its extraordinary modification in the lichenized state. Biblioth Lichenol 58:433–438Google Scholar
  109. White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. Academic, San Diego, pp 315–322Google Scholar
  110. Wiklund E, Wedin M (2003) The phylogenetic relationships of the cyanobacterial lichens in the Lecanorales suborder Peltigerineae. Cladistics 19:419–431CrossRefGoogle Scholar
  111. Wirth V (1995) Flechtenflora. Bestimmung und ökologische Kennzeichnung der Flechten Südwestdeutschlands und angrenzender Gebiete. Ulmer, StuttgartGoogle Scholar
  112. Won H, Renner SS (2005) The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum. Mol Phylogenet Evol 36:581–597PubMedCrossRefGoogle Scholar

Copyright information

© Mushroom Research Foundation 2013

Authors and Affiliations

  • Bibiana Moncada
    • 1
  • Robert Lücking
    • 2
  • Alejandra Suárez
    • 1
  1. 1.Licenciatura en BiologíaUniversidad Distrital Francisco José de CaldasHerbarioColombia
  2. 2.Department of BotanyThe Field MuseumChicagoUSA

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