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Endophytic fungi in European aspen (Populus tremula) leaves—diversity, detection, and a suggested correlation with herbivory resistance

Abstract

Fungal endophytes are found in most seed plants, but their ecological function mainly remains elusive, except in pooid (or clavicipitalean) systems. The diversity and dynamics of endophytes in non clavicipitalean plants make studies of their ecological function challenging. This paper describes the advantage of using molecular techniques to survey the ecological function of endophytes in Populus tremula clones. About 1,000 endophyte isolates were distinguished using traditional methods; these isolates represented approximately 100 morphologically distinct groups. We generated 73 DNA-sequences (18S and ITS rDNA) from these groups and determined 33 distinct taxa. They represented the Basidiomycota and Ascomycota, including diverse Sordariomycetes and Dothideomycetes, and three sequences that were identified, by their ITS sequences, as a species of Epicoccum (a genus of supposedly unknown relation within the Ascomycota) were placed within the Pleosporales, in the 18S phylogeny. Primer pairs were designed for eleven of the fungi. Of these, three primers produced bands for a subset of Aspen samples. The primer pairs allowed endophytes in field samples to be readily identified, with a detection limit of 0.15 percent fungal DNA. The presence of fungi in Aspen clones was related to field damage by herbivores and the pathogen Venturia tremula. A negative association was found in two separate surveys between Aureobasidium sp. and herbivore damage, but we found no evidence that endophyte presence was related to a history of Venturia symptoms. This approach promises to enhance greatly the scope for qualitative and quantitative detection of endophyte communities, and to improve our ability to elucidate the ecological function of non clavicipitalean endophytes.

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Acknowledgements

This study was inspired by a workshop held by Dr. Mark Vicari in Umeå in 2005 financed by the Edlunds Brothers foundation to BRA. Financial support was received from the Swedish Research Council, the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (MW and SJ) and the Kempe foundation (BRA and SJ).

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Correspondence to Benedicte R. Albrectsen.

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Suppl. Table 2

Summary of endophyte names for isolates in aspen 14/1 2008 (classification according to AFTOL) (DOC 42 kb)

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Albrectsen, B.R., Björkén, L., Varad, A. et al. Endophytic fungi in European aspen (Populus tremula) leaves—diversity, detection, and a suggested correlation with herbivory resistance. Fungal Diversity 41, 17–28 (2010). https://doi.org/10.1007/s13225-009-0011-y

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Keywords

  • Aureobasidium
  • Primer design
  • Species richness
  • Phylogeny
  • Seasonality
  • Gene activity
  • Populus tremula
  • Non clavicipitalean system