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Study of Effect of Vitamin D Supplementation on the Clinical, Hormonal and Metabolic Profile of the PCOS Women

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Insulin resistance is one of the most common features of polycystic ovary syndrome, and some studies suggest that vitamin D deficiency may have role in insulin resistance.


To study the effect of vitamin D supplementation on the clinical, hormonal and metabolic profile of the PCOS women.

Study Design

Randomized, placebo-controlled, interventional, double-blind study.

Materials and Methods

PCOS women were evaluated and enrolled after considering inclusion and exclusion criteria. They were randomized by block randomization with sealed envelope system done in two groups. In the study group (n = 25), patients were supplemented with vitamin D 60,000 IU weekly for 12 weeks, whereas control group (n = 25) was given placebo weekly for the same period. Both the groups were compared pre- and post-supplementation for variables like clinical profile, biochemical profile and metabolic profile. Statistical analysis was performed by the SPSS program for Windows, version 10.1 (SPSS, Chicago, IL).


In the study (n = 50), PCOS patients were enrolled; 34 patients (68%) were vitamin D deficient (≤20 ng/ml) out of which 10 patients (29%) were severely deficient (<10 ng/ml). Twelve patients (24%) were vitamin D insufficient showing high prevalence of vitamin D deficiency in the PCOS women. The difference in mean serum fasting glucose pre- and post-supplementation of vitamin D in study group was found to be statistically significant with p value of 0.041. There was significant difference seen in insulin resistance (IR) (2.38 ± 4.88–1.00 ± 0.58, p = 0.003), serum fasting insulin (10.34 ± 20.00–5.00 ± 3.25, p = 0.021), and increase in insulin sensitivity determined by QUICKI (0.37 ± 0.04–0.394 ± 0.009, p = 0.001) after supplementation with vitamin D.


The study concluded that there was a beneficial effect of vitamin D supplementation on ovulatory dysfunctions and blood pressure. Post-supplementation, there were decrease in insulin resistance and increase in insulin sensitivity. In the study decreased serum fasting insulin level and fasting blood sugar after vitamin D supplementation suggest underlying role of vitamin D in glucose homeostasis.

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This study was not funded by any outsource.

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Correspondence to Nupur Gupta.

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All authors declare that they have no conflict of interest.

Research Involving Human Participants

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Taru Gupta is Professor and Academic Head, Department of Obstetrics and Gynaecology, ESI PGIMSR Basaidarapur, New Delhi, India; Mukta Rawat is a Postgraduate Student in Department of Obstetrics and Gynaecology, ESI PGIMSR Basaidarapur, New Delhi, India; Nupur Gupta is a Assistant Professor, Obstetrics and Gynaecology, ESI PGIMSR Basaidarapur, New Delhi, India; Sarika Arora is Professor, Biochemistry, ESI PGIMSR Basaidarapur, New Delhi, India.

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Gupta, T., Rawat, M., Gupta, N. et al. Study of Effect of Vitamin D Supplementation on the Clinical, Hormonal and Metabolic Profile of the PCOS Women. J Obstet Gynecol India 67, 349–355 (2017).

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