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First outbreak reported caused by Erysipelothrix species strain 2 in turkeys from poultry-producing farms in Brazil

Annals of Microbiology volume 69, pages 1211–1215 (2019)Cite this article

Abstract

Purpose

The aims of this study were to report the first isolation of Erysipelothrix sp. strain 2 (ES2) associated with clinical signs of diseases as well as mortality in turkeys and identify the antimicrobial resistance of the isolates.

Methods

We evaluated 118 farms for bacteriological analysis and TaqMan real-time PCR to identify the microorganism in different organs. After this, we made the epidemiological analysis between the positive flocks and the mortality mean. We performed the sequencing of the 16S rRNA region and the assessment of antimicrobial resistance.

Results

We have identified 18 (15.25%) as ES2-positive flocks, without any other species from the same genus being found. After analysing the organ samples, we found liver as the organ of choice for the isolation of the ES2. The sequencing of 16S rRNA region of ES2 identified high homology with E. tonsillarum and E. rhusiopathiae, suggesting that it is not the best-suited target to identify this species. We have found a positive association between isolation of the bacteria in organs and flocks’ mortality. Positive flocks had a mortality mean rate of 6.87%, which is significantly greater than 3.76% in negative flocks. Ill turkeys had gross lesions of generalized septicaemia. The bacterial isolates showed high resistance to fosfomycin and trimethoprim/sulfamethoxazole and sensibility to norfloxacin, amoxicillin and lincomycin/spectinomycin.

Conclusion

This is the first study in the world that addressed ES2 as the causative agent of erysipelas in turkey.

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Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for their financial support.

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Authors and Affiliations

  1. Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, 38402-018, Brazil

    Patrícia G. Hoepers, Thais F. M. dos Reis, Daise A. Rossi, Edson C. V. Junior & Belchiolina B. Fonseca

  2. Universidade de Uberaba (UNIUBE), Uberaba, MG, 38055-500, Brazil

    Eliane P. Mendonça

  3. Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Priscilla K. Koerich

  4. BRF Brasil Food, São Paulo, Brazil

    Tiago V. J. França

  5. UDESC-Universidade do estado de Santa Catarina, Cedima, Centro de Diagnóstico de Microbiologia Animal, Florianopolis, SC, 88035-901, Brazil

    João P. Zuffo

Authors
  1. Patrícia G. Hoepers
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  2. Thais F. M. dos Reis
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  3. Eliane P. Mendonça
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  4. Daise A. Rossi
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  5. Priscilla K. Koerich
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  6. Tiago V. J. França
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  7. João P. Zuffo
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  8. Edson C. V. Junior
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  9. Belchiolina B. Fonseca
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Corresponding author

Correspondence to Belchiolina B. Fonseca.

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Conflicts of interest

The authors declare that they have no conflict of interests.

Research involving human animals

The necropsied birds of this work were naturally and recently dead, that by Brazilian Law No. 11,794 of October 8, 2008, there is no need for an evaluation by the Ethics Committee in animal research (because it deals with the handling of dead animals). Even so, we have communicated the Committee on Ethics in the Use of Animals of the Federal University of Uberlandia that certified our work with the number A004/19.

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Hoepers, P.G., dos Reis, T.F.M., Mendonça, E.P. et al. First outbreak reported caused by Erysipelothrix species strain 2 in turkeys from poultry-producing farms in Brazil. Ann Microbiol 69, 1211–1215 (2019). https://doi.org/10.1007/s13213-019-01505-3

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  • DOI: https://doi.org/10.1007/s13213-019-01505-3

Keywords

  • Poultry
  • Erysipelas
  • Septicaemia
  • 16S rRNA region sequencing
  • Antimicrobial resistance