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Optimization of date syrup as a novel medium for lovastatin production by Aspergillus terreus ATCC 20542 and analyzing assimilation kinetic of carbohydrates

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Abstract

Lovastatin is a statin drug, which lowers cholesterol level in blood due to inhibition of (S)-3-hydroxy-3-methylglutaryl-CoA reductase. Date syrup is a rich medium for microbial growth and metabolite production. The main carbohydrates present in the date syrup are glucose and fructose. In this study, date syrup was used as a complex and bioresource medium for lovastatin production by Aspergillus terreus in the submerged cultivation. Optimization of the date syrup medium in order to achieve the highest titers of lovastatin and biomass was carried out. Four factors were studied by response surface methodology including concentration of date syrup carbohydrates, yeast extract concentration, pH, and rotation speed of the shaker. Optimal conditions for these factors found were as follows: concentration of date syrup carbohydrates, 64 g/l; yeast extract concentration, 15 g/l; pH, 6.5; and agitation speed, 150 rpm. It gave lovastatin concentration of 105.6 mg/l. Next, batch cultures in the optimal conditions were performed in a 2.5-l working volume bioreactor and led to the lovastatin titer of 241.1 mg/l during 12 days. Aspergillus terreus showed diauxic growth in the optimized medium with a shift from glucose to fructose assimilation during the run. Glucose and fructose assimilation kinetic parameters revealed that more lovastatin is produced during glucose assimilation, while more biomass was formed during fructose assimilation.

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Acknowledgements

The authors would you like to thank Dr. Hosseini from Nano biotechnology division of university of Tehran for providing facilities in experiments analysis and also Roial technology group. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. School of Advanced Technologies in Medicine, Department of Medical Nanotechnology, Tehran University of Medical Science, Tehran, Iran

    Farshid Jaberi Ansari

  2. Faculty of New Sciences and Technologies, Department of Life Science Engineering, University of Tehran, North kargar St., P.O. Box: 14395-1561, Tehran, Iran

    Hassan Jalili

  3. Department of Bioprocess Engineering, Technical University of Lodz, ul. Wolczanska 213, 90-924, Lodz, Poland

    Marcin Bizukojc

  4. Ecole Nationale Supérieure de Chimie de Rennes / Université de Rennes 1, CNRS, UMR 6226, 11 allée de Beaulieu, CS 50837, 35708, Rennes, France

    Abdeltif Amrane

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  1. Farshid Jaberi Ansari
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  2. Hassan Jalili
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  4. Abdeltif Amrane
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Correspondence to Hassan Jalili.

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Ansari, F.J., Jalili, H., Bizukojc, M. et al. Optimization of date syrup as a novel medium for lovastatin production by Aspergillus terreus ATCC 20542 and analyzing assimilation kinetic of carbohydrates. Ann Microbiol 68, 351–363 (2018). https://doi.org/10.1007/s13213-018-1342-2

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  • DOI: https://doi.org/10.1007/s13213-018-1342-2

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