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Annals of Microbiology

, Volume 68, Issue 5, pp 295–304 | Cite as

Characterization of As(III) oxidizing Achromobacter sp. strain N2: effects on arsenic toxicity and translocation in rice

  • Anna Corsini
  • Milena Colombo
  • Claudio Gardana
  • Sarah Zecchin
  • Paolo Simonetti
  • Lucia Cavalca
Original Article
  • 109 Downloads

Abstract

Achromobacter sp. strain N2 was isolated from a pyrite-cinder-contaminated soil and presented plant growth promoting traits (ACC deaminase activity, production of indole-3-acetic and jasmonic acids, siderophores secretion, and phosphate solubilization) and arsenic transformation abilities. Achromobacter sp. strain N2 was resistant to different metals and metalloids, including arsenate (100 mM) and arsenite (5 mM). The strain was resistant to ionic stressors (i.e., arsenate and NaCl), whereas bacterial growth was impaired by osmotic stress. Strain N2 was able to oxidize 1.0 mmol L−1 of arsenite to arsenate in 72 h. This evidence was supported by the retrieval of an arsenite oxidase AioA gene highly homologous to arsenite oxidases of Achromobacter and Alcaligenes species. Rice seeds of Oryza sativa (var. Loto) were bio-primed with ACCD-induced and non-induced cells in order to evaluate the effect of inoculation on rice seedlings growth and arsenic uptake. The bacterization with ACCD-induced cells significantly improved seed germination and seedling heights if compared with the seeds inoculated with non-induced cells and non-primed seeds. Enhanced arsenic uptake was evidenced in the presence of ACCD-induced cells, suggesting a role of ACCD activity on the mitigation of the toxicity of arsenic accumulated by the plant. This kind of responses should be taken into account when proposing PGP strains for improving plant growth in arsenic-rich soils.

Keywords

Arsenic Arsenite oxidase Plant growth promotion ACC deaminase Rice 

Notes

Funding information

The research was supported by Ministry of University and Research program PRIN (2010JBNLJ7-004).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  1. 1.Department of Food, Environmental and Nutritional Sciences—DeFENSUniversità degli Studi di MilanoMilanItaly

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