The response of LuxS/AI-2 quorum sensing in Lactobacillus fermentum 2-1 to changes in environmental growth conditions
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Quorum sensing (QS) is a communication mechanism based on the production of autoinducers in order to regulate cooperative behavior and physiological traits among bacteria. Autoinducer-2 (AI-2) is mediated by luxS and pfs genes and is used for both intra- and interspecies communication. The effects of environmental stresses, such as pH, temperature, osmotic, and nutrient stresses, were investigated on the LuxS/AI-2 QS system in Lactobacillus fermentum 2-1. The AI-2 activity in L. fermentum 2-1 was induced by stress responses to acid shock, high temperature, and nutrient inadequacy while a decrease in activity was correlated with high pH, low temperature, and osmotic stress. Maximum transcription of luxS and pfs was achieved after L. fermentum 2-1 was exposed to acid shock, low temperature, high concentration of NaCl, and diluted MRS broth. The results indicate that various stresses affected the LuxS/AI-2 QS system, which may be involved in the resistance process of the L. fermentum 2-1.
KeywordsQuorum sensing Lactobacillus fermentum 2-1 Autoinducer-2 Transcription Environmental stresses
This project was supported by the Program of National Natural Science Foundation of China (No. 31360396).
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Conflict of interest
The authors declare that they have no conflict of interest.
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