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Diversity and characterization of Azotobacter isolates obtained from rice rhizosphere soils in Taiwan

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Abstract

Azotobacter species, free-living nitrogen-fixing bacteria, have been used as biofertilizers to improve the productivity of non-leguminous crops, including rice, due to their various plant growth-promoting traits. The purposes of this study were to characterize Azotobacter species isolated from rice rhizospheres in Taiwan and to determine the relationship between the species diversity of Azotobacter and soil properties. A total of 98 Azotobacter isolates were isolated from 27 paddy fields, and 16S rRNA gene sequences were used to identify Azotobacter species. The characteristics of these Azotobacter strains were analyzed including carbon source utilization and plant growth-promoting traits such as nitrogen fixation activity, indole acetic acid production, phosphate-solubilizing ability, and siderophore secretion. Of the 98 strains isolated in this study, 12 were selected to evaluate their effects on rice growth. Four species of Azotobacter were identified within these 98 strains, including A. beijerinckii, A. chroococcum, A. tropicalis, and A. vinelandii. Of these four species, A. chroococcum was predominant (51.0%) but A. beijerinckii had the highest level of nucleotide diversity. Strains within individual Azotobacter species showed diverse profiles in carbon source utilization. In addition, the species diversity of Azotobacter was significantly related to soil pH, Mn, and Zn. Members of the same Azotobacter species showed diverse plant growth-promoting traits, suggesting that the 98 strains isolated in this study may not equally effective in promoting rice growth. Of the 12 strains evaluated, A. beijerinckii CHB 461, A. chroococcum CHB 846, and A. chroococcum CHB 869 may be used to develop biofertilizers for rice cultivation because they significantly promoted rice growth. This study contributes to the selection of suitable Azotobacter strains for developing biofertilizer formulations and soil management strategies of Azotobacter for paddy fields.

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Acknowledgments

This research was supported by a grant from the Ministry of Science and Technology, Taiwan, R.O.C. (NSC 102-2313-B-005-034-MY2) to C. -H. Huang. We thank R.-F. Wang and S.-R. Ye for their technical assistance.

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Authors and Affiliations

  1. Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung, Taiwan

    Syuan-Lu Chen, Meng-Ke Tsai, Yuh-Ming Huang & Cheng-Hua Huang

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  1. Syuan-Lu Chen
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  2. Meng-Ke Tsai
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Correspondence to Cheng-Hua Huang.

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Chen, SL., Tsai, MK., Huang, YM. et al. Diversity and characterization of Azotobacter isolates obtained from rice rhizosphere soils in Taiwan. Ann Microbiol 68, 17–26 (2018). https://doi.org/10.1007/s13213-017-1312-0

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  • DOI: https://doi.org/10.1007/s13213-017-1312-0

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