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Annals of Microbiology

, Volume 67, Issue 5, pp 359–369 | Cite as

RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato

  • Veerubommu ShanmugamEmail author
  • Vandana Sharma
  • Poonam Bharti
  • Poonam Jyoti
  • Sudesh Kumar Yadav
  • Rashmi Aggarwal
  • Shekhar Jain
Original Article

Abstract

The necessity to develop new strategies for the control of Fusarium wilt of tomato signifies the identification of pathogencity genes and ascertaining their role to use them as molecular tools for fungicide development or to develop transgenics. Semi-quantitative gene expression studies have identified two pathogenicity genes, FOW2 and chsV, reported as ZnII)2Cys6-type transcription regulator and class V chitin synthase, respectively, as potential ones for being secreted all the time. The roles of these genes in the pathogenicity of Fusarium oxysporum and F. solani have been established by RNA interference (RNAi)-induced silencing (knockdown). The silencing vector encoding hairpin RNA of each of the gene fragment was constructed in a two-step PCR-based cloning, and introduced into the fungal genomic DNA. Silencing of either of the genes resulted in less virulent fungal phenotypes with altered physiological characteristics like sporulation and growth on solid media and a reduction in mRNA expression. The results therefore demonstrate the applicability of these pathogenicity genes as useful molecular targets for exploitation in Fusarium wilt control in tomato.

Keywords

RNAi Silencing FOW2 chsV Fusarium solani Tomato 

Notes

Acknowledgements

The authors are grateful to the Director, CSIR-Institute of Himalayan Bioresource Technology, Palampur and Director, ICAR-Indian Agricultural Research Institute, for support and encouragement during the course of this investigation. This work was supported by Department of Biotechnology, Government of India and Indian Council of Agricultural Research through the Phytofura programme.

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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2017

Authors and Affiliations

  • Veerubommu Shanmugam
    • 1
    Email author
  • Vandana Sharma
    • 2
  • Poonam Bharti
    • 3
    • 4
  • Poonam Jyoti
    • 3
  • Sudesh Kumar Yadav
    • 3
  • Rashmi Aggarwal
    • 1
  • Shekhar Jain
    • 1
  1. 1.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia
  2. 2.Indian Institute of TechnologyRoorkeeIndia
  3. 3.Plant Metabolic Engineering Laboratory, Biotechnology DivisionCSIR-Institute of Himalayan Bioresource TechnologyPalampurIndia
  4. 4.Academy of Scientific and Innovative ResearchNew DelhiIndia

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