Characterization of fructophilic lactic microbiota of Apis mellifera from the Caucasus Mountains
- 332 Downloads
Microbial symbionts of honeybee colony are considered as promising tools to support the honeybee population welfare. The majority of existing honeybee microbiota studies is focused on genetic description of the honeybee-associated microbiome fingerprints. The lack of a deeper knowledge on the bacterial community colonizing the honeybee niche, which may be helpful in encouraging industrial applications of this microbiota, led us to undertake this study. The biodiversity of the cultivable fructophilic lactic acid bacteria (FLAB) isolated from adult honeybee intestine and beebread samples was studied. Phenotypic properties of probiotic interest, such as the adhesive potential using in vitro models and adhesion determinants, were also investigated. Antibiotic resistance profiles as reliable markers to evaluate the impact of long-term and current exposure of honeybees to antibiotics were phenotypically determined on the isolated lactic acid bacteria (LAB). The mannose-specific adhesion and high cell surface hydrophobicity found in the studied FLAB isolates sheds light on the effective adaptation of microbiota to specific ecologic niche. It is the first report of phenotypically detected antibiotic resistance profiles of honeybee endogenous bacteria and the first account of minimum inhibitory concentration (MIC) values for four antibiotics used in beekeeping practice.
KeywordsFructophilic lactic acid bacteria Honeybees Microbial adhesion Caucasus
The author I.J. would like to express his gratitude to the Shota Rustaveli National Science Foundation (SRNSF) for partial support allowing his PhD training in Italy. The author also expresses his gratitude to the Service of Science and Technology of the French Embassy in Tbilisi for the fellowship allowing his PhD training in France.
- Giraffa G, Neviani E (2000) Molecular identification and characterization of food-associated lactobacilli. Ital J Food Sci 12:403–423Google Scholar
- Gurgulova K, Panchev I, Stanchev P (2003) A study on antibacterial activity of Riphampizyn against bee disease microorganisms. Uludag Bee J 11:40–41Google Scholar
- Mercenier A, Lenoir-Wijnkoop I, Sanders ME (2008) Physiological and functional properties of probiotics. Bull Int Dairy Fed 429:2–6Google Scholar
- Murray KD, Aronstein KA, Eischen F (2009) Promiscuous DNA and terramycin resistance in American foulbrood bacteria. Am Bee J 149:577–581Google Scholar
- Pilatic H (2012) Pesticides and honey bees. State of the Science. Report from the Pesticide Action Network North AmericaGoogle Scholar
- Salminen S, van Loveren H (2012) Probiotics and prebiotics: health claim substantiation. Microb Ecol Health Dis 23:18568Google Scholar
- Tirado R, Simon G, Johnston P (2013) Bees in decline. A review of factors that put pollinators and agriculture in Europe at risk. Greenpeace Research Laboratories Technical Report (Review)Google Scholar
- Vauterin L, Vauterin P (1992) Computer-aided objective comparison of electrophoresis patterns for grouping and identification of microorganisms. Eur Microbiol 1:37–41Google Scholar
- Wu M, Sugimura Y, Taylor D, Yoshiyama M (2013) Honeybee gastrointestinal bacteria for novel and sustainable disease control strategies. J Dev Sust Agric 8:85–90Google Scholar