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Lactobacilli possess inhibitory activity against dipeptidyl peptidase-4 (DPP-4)

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Abstract

Dipeptidyl peptidase-4 (DPP-4) plays an important role in the enzymatic inactivation of incretin hormones. In this context, drugs that inhibit DPP-4 have been developed and clinically approved as therapies for type 2 diabetes. As the primary substrates of DPP-4 are produced in the intestinal lining, we investigated whether lactobacilli colonizing the gut could inhibit this enzyme. Fifteen Lactobacillus strains (Lb 1–15) from human infant faecal samples were isolated, identified, extracted and screened for inhibitory activity against DPP-4. Activity was compared against Lactobacillus reference strains (Ref 1–7), a Gram-positive control (Ctrl 1) and two Gram-negative controls (Ctrl 2–3). A range of DPP-4 inhibitory activity was observed (10–32 %; p < 0.05–0.001). Strains of L. plantarum (12–25 %) and L. fermentum (14 %) had significant inhibitory activity. However, we noted that Escherichia coli (Ctrl 2) and Salmonella Typhimurium (Ctrl 3) had the greatest inhibitory activity (30–32 %). Contrastingly, some isolates (Lb 12–15) and reference cultures (Ref 1–4), instead of inhibiting DPP-4, actually enhanced it, perhaps indicating the presence of X-prolyl-dipeptidyl-amino-peptidase (PepX). This provides a future rationale for using probiotic bacteria or their components for management of type 2 diabetes via DPP-4 inhibition.

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Acknowledgments

The authors acknowledge the PhD studentship provided to Harsh Panwar (India) as part of a 1-year Split-site Scholarship awarded by the Commonwealth Scholarship Commission (UK) (INCN-2011-43), tenable at Queen’s University Belfast (UK).

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Authors and Affiliations

  1. Advanced ASSET Centre, Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, Belfast, BT9 5AG, UK

    Harsh Panwar, Danielle Calderwood, Irene R. Grant & Brian D. Green

  2. Dairy Microbiology Division, College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

    Harsh Panwar

  3. Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India

    Sunita Grover

  4. Commonwealth Scholar, Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, Belfast, UK

    Harsh Panwar

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  1. Harsh Panwar
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  2. Danielle Calderwood
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  3. Irene R. Grant
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  4. Sunita Grover
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  5. Brian D. Green
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Correspondence to Brian D. Green.

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Panwar, H., Calderwood, D., Grant, I.R. et al. Lactobacilli possess inhibitory activity against dipeptidyl peptidase-4 (DPP-4). Ann Microbiol 66, 505–509 (2016). https://doi.org/10.1007/s13213-015-1129-7

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  • DOI: https://doi.org/10.1007/s13213-015-1129-7

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