Skip to main content
SpringerLink
Log in

Incidence of Arcobacter spp. in fresh seafood from retail markets in Mumbai, India

  • Original Article
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

The occurrence of Campylobacter-like bacteria in fresh fish sold in retail markets of Mumbai was studied. Microaerophilic bacteria isolated following enrichment in Bolton broth and selective plating on Preston blood agar were subjected to biochemical identification followed by polymerase chain reaction to detect Campylobacter, Arcobacter and Helicobacter genera. Arcobacter spp. was isolated from 10 (25 %) of the 40 seafood samples analysed. Based on the comparison of partial 16S rDNA gene sequence, the Arcobacter isolates of this study were identified as Arcobacter butzleri, A. skirrowii and A. cibarius, with A. butzleri being the predominant species. The presence of arcobacter represents a health risk and requires further study to determine the sources of contamination of seafood and the pathogenic potential of Arcobacter spp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Anderson KF, Kiehlbauch JA, Anderson DC, McClure HM, Wachsmuth IK (1993) Arcobacter (Campylobacter) butzleri-associated diarrheal illness in a nonhuman primate population. Infect Immun 61:2220–2223

    PubMed Central  PubMed  CAS  Google Scholar 

  • Collado L, Figueras MJ (2011) Taxonomy, epidemiology, and clinical relevance of the genus Arcobacter. Clin Microbiol Rev 24:174–192

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Collado L, Guarro J, Figueras MJ (2009) Prevalence of Arcobacter in meat and shellfish. J Food Prot 72:1102–1106

    PubMed  Google Scholar 

  • De Boer E, Tilburg JJ, Woodward DL, Lior H, Johnson WM (1996) A selective medium for the isolation of Arcobacter from meats. Lett Appl Microbiol 23:64–66

    Article  PubMed  Google Scholar 

  • Dore MP, Sepulveda AR, El-Zimaity H, Yamaoka Y, Osato MS, Mototsugu K, Nieddu AM, Realdi G, Graham DY (2001) Isolation of Helicobacter pylori from sheep—implications for transmission to humans. Am J Gastroenterol 96:1396–1401

    PubMed  CAS  Google Scholar 

  • Ellis WA, Neill SD, O’Brien JJ, Ferguson HW, Hanna J (1977) Isolation of Spirillum/Vibrio-like organisms from bovine fetuses. Vet Rec 100:451–452

    Article  PubMed  CAS  Google Scholar 

  • Fera MT, Maugeri TL, Gugliandolo C, Beninati C, Giannone M, La Camera E, Carbone M (2004) Detection of Arcobacter spp. in the coastal environment of the Mediterranean Sea. Appl Environ Microbiol 70:1271–1276

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • González I, García T, Antolín A, Hernández PE, Martín R (2000) Development of a combined PCR-culture technique for the rapid detection of Arcobacter spp. in chicken meat. Lett Appl Microbiol 30:207–212

    Article  PubMed  Google Scholar 

  • Griggs DJ, Peake L, Johnson MM, Ghori S, Mott A, Piddock LJV (2009) Beta-lactamase-mediated beta-lactam resistance in Campylobacter species: prevalence of Cj0299 (bla OXA-61) and evidence for a novel beta-Lactamase in C. jejuni. Antimicrob Agents Chemother 53:3357–3364

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Harmon KM, Wesley IV (1996) Identification of Arcobacter isolates by PCR. Lett Appl Microbiol 23:241–244

    Article  PubMed  CAS  Google Scholar 

  • Ho HTK, Lipman LJA, Gaastra W (2006) Arcobacter, what is known and unknown about a potential foodborne zoonotic agent! Vet Microbiol 115:1–13

    Article  PubMed  Google Scholar 

  • ICMSF (International Commission on Microbiological Specifications for Foods) (2002) Microorganisms in foods 7- microbial testing in food safety management. Kluwer/Plenum, New York

    Book  Google Scholar 

  • Jiang ZD, Dupont HL, Brown EL, Nandy RK, Ramamurthy T, Sinha A, Ghosh S, Guin S, Gurleen K, Rodrigues S, Chen JJ, McKenzie R, Steffen R (2010) Microbial etiology of travelers’ diarrhea in Mexico, Guatemala, and India: importance of enterotoxigenic Bacteroides fragilis and Arcobacter species. J Clin Microbiol 48:1417–1419

    Article  PubMed Central  PubMed  Google Scholar 

  • Johnson LG, Murano EA (1999) Comparison of three protocols for the isolation of Arcobacter from poultry. J Food Prot 62:610–614

    PubMed  CAS  Google Scholar 

  • Kabeya H, Maruyama S, Morita Y, Kubo M, Yamamoto K, Arai S, Izumi T, Kobayashi Y, Katsube Y, Mikami T (2003) Distribution of Arcobacter species among livestock in Japan. Vet Microbiol 93(2):153–158

  • Kiehlbauch JA, Brenner DJ, Nicholson MA, Baker CN, Patton CM, Steigerwalt AG, Wachsmuth IK (1991) Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. J Clin Microbiol 29:376–385

  • Kownhar H, Shankar EM, Rajan R, Vengatesan A, Rao UA (2007) Prevalence of Campylobacter jejuni and enteric bacterial pathogens among hospitalized HIV infected versus non-HIV infected patients with diarrhoea in southern India. Scand J Infect Dis 39:862–866

    Article  PubMed  Google Scholar 

  • Lee C, Agidi S, Marion JW, Lee J (2012) Arcobacter in Lake Erie beach waters: an emerging gastrointestinal pathogen linked with human-associated fecal contamination. Appl Environ Microbiol 78:5511–5519

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Lerner J, Brumberger V, Preac-Mursic V (1994) Severe diarrhea associated with Arcobacter butzleri. Eur J Clin Microbiol 13:660–662

    Article  CAS  Google Scholar 

  • Levican A, Figueras MJ (2013) Performance of five molecular methods for monitoring Arcobacter spp. BMC Microbiol 13:220

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Levican A, Collado L, Aguilar C, Yustes C, Diéguez AL, Romalde JL, Figueras MJ (2012) Arcobacter bivalviorum sp. nov. and Arcobacter venerupis sp. nov., new species isolated from shellfish. Syst Appl Microbiol 35:133–138

    Article  PubMed  CAS  Google Scholar 

  • Levican A, Collado L, Figueras MJ (2013) Arcobacter cloacae sp. nov. and Arcobacter suis sp. nov., two new species isolated from food and sewage. Syst Appl Microbiol 36:22–27

    Article  PubMed  CAS  Google Scholar 

  • Levican A, Collado L, Yustes C, Aguilar C, Figueras MJ (2014) Higher water temperature and incubation under aerobic and microaerobic conditions increase the recovery and diversity of Arcobacter spp. from shellfish. Appl Environ Microbiol 80:385–389

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Linton D, Lawson AJ, Owen RJ, Stanley J (1997) PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. J Clin Microbiol 35:2568–2572

    PubMed Central  PubMed  CAS  Google Scholar 

  • Marshall BJ, Warren JR (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1:1311–1315

    Article  PubMed  CAS  Google Scholar 

  • Marshall SM, Melito PL, Woodward DL, Johnson WM, Rodgers FG, Mulvey MR (1999) Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rDNA gene. J Clin Microbiol 37:4158–4160

    PubMed Central  PubMed  CAS  Google Scholar 

  • Merga JY, Royden A, Pandey AK, Williams NJ (2014) Arcobacter spp. isolated from untreated domestic effluent. Lett Appl Microbiol 59:122–126

    Article  PubMed  CAS  Google Scholar 

  • Morita Y, Maruyama S, Kabeya H, Boonmar S, Nimsuphan B, Nagai A, Kozawa K, Nakajima T, Mikami T, Kimura H (2004) Isolation and phylogenetic analysis of Arcobacter spp. in ground chicken meat and environmental water in Japan and Thailand. Microbiol Immunol 48:527–533

    Article  PubMed  CAS  Google Scholar 

  • Neill SD, Campbell JN, O’brien JJ, Weatherup STC, Ellis WA (1985) Taxonomic position of Campylobacter cryaerophila sp. nov. Int J Syst Bacteriol 35:342–356

    Article  Google Scholar 

  • Nieva-Echevarria B, Martinez-Malaxetxebarria I, Girbau C, Alonso R, Fernández-Astorga A (2013) Prevalence and genetic diversity of Arcobacter in food products in the north of Spain. J Food Prot 76:1447–1450

    Article  PubMed  Google Scholar 

  • On SL (2001) Taxonomy of Campylobacter, Arcobacter, Helicobacter and related bacteria: current status, future prospects and immediate concerns. Symp Ser Soc Appl Microbiol 30:1S–15S

  • Patyal A, Rathore RS, Mohan HV, Dhama K, Kumar A (2011) Prevalence of Arcobacter spp. in humans, animals and foods of animal origin including sea food from India. Transbound Emerg Dis 58:402–410

    Article  PubMed  CAS  Google Scholar 

  • Pejchalová M, Dostalíková E, Slámová M, Brozková I, Vytrasová J (2008) Prevalence and diversity of Arcobacter spp. in the Czech Republic. J Food Prot 71:719–727

    PubMed  Google Scholar 

  • Rice EW, Rodgers MR, Wesley IV, Johnson CH, Tanner SA (1999) Isolation of Arcobacter butzleri from ground water. Lett Appl Microbiol 28:31–35

    Article  PubMed  CAS  Google Scholar 

  • Schroeder-Tucker L, Wesley IV, Kiehlbauch JA, Larson DJ, Thomas LA, Erickson GA (1996) Phenotypic and ribosomal RNA characterization of Arcobacter species isolated from porcine aborted fetuses. J Vet Diagn Investig 8:186–195

    Article  CAS  Google Scholar 

  • Scullion R, Harrington CS, Madden RH (2006) Prevalence of Arcobacter spp. in raw milk and retail raw meats in Northern Ireland. J Food Prot 69:1986–1990

    PubMed  Google Scholar 

  • Taylor DN, Kiehlbauch JA, Tee W, Pitarangsi C, Echeverria P (1991) Isolation of group 2 aerotolerant Campylobacter species from Thai children with diarrhea. J Infect Dis 163:1062–1067

    Article  PubMed  CAS  Google Scholar 

  • Van Driessche E, Houf K (2007) Characterization of the Arcobacter contamination on Belgian pork carcasses and raw retail pork. Int J Food Microbiol 118:20–26

    Article  PubMed  CAS  Google Scholar 

  • Vandamme P, Falsen E, Rossau R, Hoste B, Segers P, Tytgat R, De Ley J (1991) Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 41:88–103

    Article  PubMed  CAS  Google Scholar 

  • Vandamme P, Harrington CS, Jalava K, On SL (2000) Misidentifying helicobacters: the Helicobacter cinaedi example. J Clin Microbiol 38:2261–2266

    PubMed Central  PubMed  CAS  Google Scholar 

  • Yan JJ, Ko WC, Huang AH, Chen HM, Jin YT, Wu JJ (2000) Arcobacter butzleri bacteremia in a patient with liver cirrhosis. J Formos Med Assoc 99:166–169

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank the Director & Vice-Chancellor, Central Institute of Fisheries Education (CIFE), Mumbai for help and suggestions.

Author information

Authors and Affiliations

  1. QC Laboratory, Post Harvest Technology Department, Central Institute of Fisheries Education (CIFE), Mumbai, 400061, India

    Srinu Rathlavath, Saurabh Mishra, Sanath Kumar & Binaya Bhusan Nayak

Authors
  1. Srinu Rathlavath
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saurabh Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sanath Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Binaya Bhusan Nayak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Binaya Bhusan Nayak.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rathlavath, S., Mishra, S., Kumar, S. et al. Incidence of Arcobacter spp. in fresh seafood from retail markets in Mumbai, India. Ann Microbiol 66, 165–170 (2016). https://doi.org/10.1007/s13213-015-1092-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13213-015-1092-3

Keywords

Search

Navigation