Skip to main content
SpringerLink
Log in

A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice

  • Original Article
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

Biodegradable polymers of polyhydroxyalkanoates (PHAs) are produced and accumulated in various microorganisms, especially in bacteria. In this work, a cheap agricultural sugar cane juice (SCJ) was used as a carbon source by Alcaligenes latus during its growth coupled with the production of PHAs via batch/ repeated batch fermentation processes. In batch fermentation, the maximum PHAs and PHAs content of 2.20 ± 0.14 g/L and 53.65 % were obtained within 24 h while in the case of four cycles of repeated batch fermentation, the PHAs productivity was reached at about 0.21 g/L·h that was 2.33 fold when compared to the first batch fermentation (0.09 g/L·h). Interestingly, the duration in each cycle or in batch operation during repeated batch fermentation was shorter than that found in the batch fermentation and PHAs productivity was also improved. After PHAs recovery, the physicochemical properties of nuclear magnetic resonance (NMR) and a fourier transform infrared (FTIR) spectrogram were characterized and revealed to be in full agreement with the standard polymer of polyhydroxybutyrate (PHB).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Braunegg G, Bogensberger B (1985) For the kinetics of growth and the storage of poly-D (−)-3-hydroxybutyric acid by Alcaligenes latus. Acta Biotechnol 5:339–345

    Article  CAS  Google Scholar 

  • El-Sayed AA, Abdelhady HM, Abdel Hafez AM, Khodair TA (2009) Batch production of polyhydroxybutyrate (PHB) by Ralstonia eutropha and Alcaligenes latus using bioreactor different culture strategies. J Appl Sci Res 5:556–564

    CAS  Google Scholar 

  • Grothe E, Moo-Young M, Chisti Y (1999) Fermentation optimization for the production of (hydroxybutyric acid) microbial thermoplastic. Enzym Microb Technol 25:132–141

    Article  CAS  Google Scholar 

  • Hangii UJ (1990) Pilot scale production PHB with Alcaligenes latus. In: Dawes EA (ed) Novel biodegradable microbial polymer. Kluwer Academic Publishers, Dordrecht, pp 65–70

    Chapter  Google Scholar 

  • Hrabak O (1992) Industrial production poly-β-hydroxybutyrate. FEMS Microbiol Rev 103:251–256

    CAS  Google Scholar 

  • Huang WC, Chen SJ, Chen TL (2008) Production of hyaluronic acid by repeated batch fermentation. Biochem Eng J 40:460–464

    Article  CAS  Google Scholar 

  • Kaewkannetra P, Tanonkeo P, Tanamool V, Imai T (2008) Biorefinery of squeeze sweet sorghum juice into value added product of biopolymer. J Biotechnol 136:412

    Article  Google Scholar 

  • Khanna S, Srivastava AK (2005a) Recent advances in microbial polyhydroxyalkanoates. Process Biochem 40:607–619

    Article  CAS  Google Scholar 

  • Khanna S, Srivastava AK (2005b) Repeated batch cultivation of Ralstonia eutropha for poly (β- hydroxybutyrate) production. Biotechnol Lett 27:1401–1403

    Article  CAS  PubMed  Google Scholar 

  • Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI (1994) Production of poly (3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol Bioeng 43:892–898

    Article  CAS  PubMed  Google Scholar 

  • Kim SW, Kim P, Lee HS, Kim JH (1996) High production of poly-β-hydroxybutyrate (PHB) from Methylobacterium organophilum under potassium limitation. Biotechnol Lett 18:25–30

    Article  CAS  Google Scholar 

  • Kumalaningsih S, Nur H, Nur A (2011) Optimization of polyhydroxyalkanoates (PHA) production from liquid bean curd waste by Alcaligenes latus bacteria. J Agric Food Technol 1:63–67

    Google Scholar 

  • Kunasundari B, Sudesh K (2011) Isolation and recovery of microbial polyhydroxyalkanoates. eXPRESS Polym Lett 5:620–634

    Article  Google Scholar 

  • Lee SY (1996a) Bacterial polyhydroxyalkanoates. Biotechnol Bioeng 49:1–14

    Article  CAS  PubMed  Google Scholar 

  • Lee SY (1996b) Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnol 14:431–438

    Article  CAS  Google Scholar 

  • Lee SY (1997) E. coli moves into the plastic age. Nat Biotechnol 15:17–18

  • Lee SY, Chang HN (1995) Production of poly (3-hydroxybutyric acid) by recombinant Escherichia coli strains: genetic and fermentation studies. Can J Microbiol 41:207–215

    Article  CAS  PubMed  Google Scholar 

  • Lee SY, Yim KS, Chang HN, Chang YK (1994) Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly (3-hydroxybutyric acid) in Escherichia coli. J Biotechnol 32:203–211

    Article  CAS  PubMed  Google Scholar 

  • Morimura S, Ling ZY, Kida K (1997) Ethanol production by repeated batch fermentation at high temperature in a molasses medium containing a high concentration of total sugar by a thermotolerant flocculating yeast with improved salt tolerance. J Ferment Bioeng 83:271–274

    Article  CAS  Google Scholar 

  • Page WJ, Cornish A (1993) Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-β-hydroxybutyrate. Appl Environ Microbiol 59:4236–4244

    PubMed Central  CAS  PubMed  Google Scholar 

  • Pandey A, Soccol CR, Nigam P, Soccol VT (2000) Biotechnological potential of agro-industrial residues. I: sugarcane bagasse. Bioresour Technol 74:69–80

    Article  CAS  Google Scholar 

  • Park YS, Toda K, Fukaya M, Okumura H, Kawamura Y (1991) Production of a high concentration acetic acid by Acetobacter aceti using a repeated fed-batch culture with cell recycling. Appl Microbiol Biotechnol 35:149–153

    CAS  Google Scholar 

  • Poirier Y, Nawrath C, Somerville C (1995) Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plant. Biotechnology (NY) 13:142–150

    Article  CAS  Google Scholar 

  • Santhanam A, Sasidharan S (2010) Microbial production of polyhydroxyalkanotes (PHA) from Alcaligenes spp. and Pseudomonas oleovorans using different carbon sources. Afr J Biotechnol 9:3144–3150

    CAS  Google Scholar 

  • Suwannasing W, Mooamart S, Keawkannetra P (2011) Yields of polyhydroxyalkanoates (PHAs) during batch fermentation of sugar cane juice by Alcaligenes latus and Alcaligenes eutrophus. J Life Sci 5:960–966

    CAS  Google Scholar 

  • Suzuki T, Yamane T, Shimizu S (1986) Mass production of poly-β-hydroxybutyric acid produced in fed-batch culture with controlled carbon/nitrogen feeding. Appl Microbiol Biotechnol 24:370–374

    Article  CAS  Google Scholar 

  • Tanamool V, Imai T, Danvirutai P, Kaewkannetra P (2013) An alternative approach to the fermentation of sweet sorghum juice into biopolymer of poly-β-hydroxyalkanoates (PHAs) by newly isolated, Bacillus aryabhattai PKV01. Biotechnol Bioprocess Eng 18:65–74

    Article  CAS  Google Scholar 

  • Yamane T (1993) Yield of poly-D-(−)-3-hydroxybytyrate from various carbon sources a theoretical study. Biotechnol Bioeng 41:165–170

    Article  CAS  PubMed  Google Scholar 

  • Yamane T, Fukunaga M, Lee YW (1996) Increased PHB productivity by high cell density fed batch culture of Alcaligenes latus, a growth-associated PHB producer. Biotechnol Bioeng 50:197–202

    Article  CAS  PubMed  Google Scholar 

  • Yezza A, Halasz A (2007) Production of polyhydroxybutyrate (PHB) by Alcaligenes latus from maple sap. Biotechnol Prod Proc Eng 77:269–274

    CAS  Google Scholar 

  • Yin P, Yahiro K, Ishigaki T, Park Y, Okabez M (1998) L(+)-Lactic acid production by repeated batch culture of Rhizopus oryzae in air lift bioreactor. J Ferment Bioeng 85:96–100

Download references

Acknowledgments

One of the authors (P. Singhaboot) would like to sincerely acknowledge the Graduate School of Khon Kaen University, Khon Kaen, Thailand for a financial contribution (The Research Fund for Supporting Lecturer to Admit a High Potential Student to Study and Research on His Expert Program : Contract project no. 531T101) and a travel abroad bursary, while P. Kaewkannetra also would like to sincerely thank the Thailand Research Fund (TRF) (Contract no.DBG 5380013) and the National Research University (NRU) Project of Khon Kaen University for matching funds.

Author information

Authors and Affiliations

  1. Graduate School of Khon Kaen University, Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand

    Pakjirat Singhaboot

  2. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand

    Pakawadee Kaewkannetra

Authors
  1. Pakjirat Singhaboot
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pakawadee Kaewkannetra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pakawadee Kaewkannetra.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Singhaboot, P., Kaewkannetra, P. A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice. Ann Microbiol 65, 2081–2089 (2015). https://doi.org/10.1007/s13213-015-1046-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13213-015-1046-9

Keywords

Search

Navigation