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Genetic relationships and virulence factors in Staphylococcus aureus isolated from raw poultry in South Brazil

Annals of Microbiology volume 65, pages 1933–1940 (2015)Cite this article

Abstract

The genetic relationships in Staphylococcus aureus isolated from raw poultry obtained from various Brazilian broiler chicken processors were analyzed using repetitive extragenic palindromic-polymerase chain reaction (rep-PCR). The distribution of accessory gene regulator (agr) groups was determined, and the presence of biofilm-associated genes and phenotypic features, including biofilm formation and proteolytic, lipolytic, and β-hemolytic activities, was assessed. Isolates were grouped into three major clusters based on rep-PCR fingerprints typed with RW3A primer. The agr group I was the most common genotype identified (86.21 %), followed by groups II (10.34 %) and III (3.45 %). All strains were positive for the sasG gene; the next most frequent genes were icaA (93.1 %) and atlA (51.72 %). Twenty-six of the 29 isolates were biofilm producers. In this study, 96.55 %, 72.41 %, and 62.06 % of the isolates displayed lipolytic, β-hemolytic, and proteolytic activity, respectively. In conclusion, the rep-PCR results suggested a clonal relationship among the S. aureus isolated from raw poultry produced by different broiler chicken processors. Our results also showed that most isolates belonged to agr group I. The presence of biofilm-forming S. aureus strains in raw poultry, their ability to harbor biofilm-associated genes, and the spoilage features that they exhibit are indicative of their pathogenic potential, and may represent a serious problem in the food processing industry.

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Acknowledgments

We thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico do Brasil (CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) of the Brazilian government for the support received.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul. Rua Sarmento Leite, n° 500-Campus Central, 90050-170, Porto Alegre, Brazil

    Jaqueline Becker Pinto, Fernanda Cristina Possamai Rossatto & Ana Paula Guedes Frazzon

  2. Department of Microbiology, The Ohio State University, 484 W 12th Avenue, Columbus, OH, 43210, USA

    Paula Dalcin Martins

Authors
  1. Jaqueline Becker Pinto
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  2. Fernanda Cristina Possamai Rossatto
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  3. Paula Dalcin Martins
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  4. Ana Paula Guedes Frazzon
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Correspondence to Ana Paula Guedes Frazzon.

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Pinto, J.B., Rossatto, F.C.P., Martins, P.D. et al. Genetic relationships and virulence factors in Staphylococcus aureus isolated from raw poultry in South Brazil. Ann Microbiol 65, 1933–1940 (2015). https://doi.org/10.1007/s13213-014-1031-8

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  • DOI: https://doi.org/10.1007/s13213-014-1031-8

Keywords

  • Staphylococcus aureus
  • Raw poultry
  • agr group
  • rep-PCR
  • Virulence factor