Abstract
The objective of this research was to saccharify cassava flour by acid-acid and acid-enzyme hydrolysis and further conversion of the resulting sugar into ethanol by fermenting with the immobilized (in Ca-alginate) cells of Saccharomyces cerevisiae. The saccharification resulted in higher total sugar recovery by acid-enzyme hydrolysis (72.88 %) than by enzyme-enzyme hydrolysis (58.1 %). Further study on ethanol production was carried out using the hydrolysate obtained from acid-enzyme hydrolysis. The growth of the yeast started in the log phage and maximum ethanol (189 ± 3.1 g ethanol/kg flour) production was achieved with 94.74 ± 2.187 % sugar conversion during the stationary phase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amerine MA, Ough CS (1984) Wine and must analysis. Wiley, New York
Bailey JE, Ollis DF (1986) Biochemical engineering fundamentals. McGraw Hill, New York
Behera S, Mohanty RC, Ray RC (2010a) Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis. Appl Energy 87:2352–2355
Behera S, Kar S, Mohanty RC, Ray RC (2010b) Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomces cerevisiae cells immobilized in agar agar and Ca-alginate matrices. Appl Energy 87:96–100
Behera S, Mohanty RC, Ray RC (2011) Ethanol production from mahula (Madhuca latifolia L.) flowers with immobilized cells of Saccharomyces cerevisiae in Luffa cylindrica L. sponge discs. Appl Energy 88:212–215
Bodalo A, Bastida J, Gomez JL, Alcarz I, Asaza ML (1996) Immobilization of Pseudomonas sp. BA2 by entrapment in calcium alginate and its application for the production of L-alanine. Enzym Microb Technol 19:176–180
John RP, Nampoothiri KM, Pandey A (2007) Production of L (+) lactic acid from cassava starch hydrolysate by immobilized Lactobacillus delbrueckii. J Basic Microbiol 47:25–30
Kar S, Ray RC (2008) Statistical optimization of α-amylase production by Streptomyces erumpens MTCC 7317 cells in calcium alginate beads using response surface methodology. Pol J Microbiol 57(1):49–57
Kar S, Chaudhury P, Ray RC (2004) Comparative study of bioethanol production from sweet potato (Ipomoea batatas L.) and cassava (Manihot esculenta Crantz.) by acid enzyme hydrolysis. In: Root and Tuber crops: nutrition, food security and sustainable environment, Proceeding of the Symposium held on 29–31 October, 2004 at Regional Centre of Central Tuber Crops Research Institute, Bhubaneswar, India, pp. 276–281
Kongkiattikajorn J, Yoonan K (2006) Conversion of cassava industry waste to fermentable sugars. In: The 2nd Int. Conf. on “Sustainable energy and environment, 21–23 November, 2006, Bangkok, Thailand
Mahadevan A, Sridhar R (1999) Methods in physiological plant pathology, 5th edn. Sivakami Publication, Madras
Nduele M, Ludwig A, Van OM (1993) The use of cassava starch in the formulation of gelatin capsules. J Pharm Belg 48:325–334
Ray RC (2004) Extracellular amylase (s) production by fungi Botryodiplodia theobromae and Rhizopus oryzae grown on cassava starch residue. J Environ Biol 25:489–495
Ray RC, Swain MR (2011) Bio-ethanol, bioplastics and other fermented industrial products from cassava starch and flour. In: Pace CM (ed) Cassava: farming, uses and economic impacts. Nova Science Publishers Inc, Hauppauge
Ray RC, Mohapatra S, Panda S, Kar S (2008) Solid substrate fermentation of cassava fibrous residue for production of α-amylase, lactic acid and ethanol. J Environ Biol 29:111–115
Roble ND, Ogbonna JC, Tanaka H (2003) L-lactic acid production from raw cassava starch in a circulating loop bioreactor with cells immobilized in loofa (Luffa cylindrica). Biotechnol Lett 25:1093–1098
Selvakumar P, Ashakumary L, Panday A (1994) Microbial fermentations with immobilized cells. J Sci Ind Res 55:443–449
Singh D, Dahiya JS, Nigam P (1995) Simultaneous raw starch hydrolysis and ethanol fermentation by glucoamylase from Rhizoctonia solani and Saccharomyces cerevisiae. J Basic Microbiol 35:117–121
Swain MR, Kar S, Sahoo AK, Ray RC (2007) Ethanol fermentation of mahula (Madhuca latifolia L.) flowers using free and immobilized yeast Saccharomyces cerevisiae. Microb Res 162:93–98
Thongchul N, Navankasattusas S, Yang ST (2010) Production of lactic acid and ethanol by Rhizopus oryzae integrated with cassava pulp hydrolysis. Bioprocess Biosyst Eng 33:407–416
Ueda S, Zenin CT, Monteiro DA, Park YK (1981) Production of ethanol from raw cassava starch by a non-conventional fermentation method. In: John Wiley and Sons Inc. Biotechnol Bioeng 23:291–299
Vijayagopal K, Balagopalan C (1989) Fermentation of cassava starch hydrolysate with immobilised cells of Saccharomyces cerevisiae. Starch-Starke 41:271–275
Vuilleumier S (1993) Worldwide production of highfructose syrup and crystalline fructose. Am J Clin Nutr 58:733S–736S
Woiciechowski AL, Nitsche S, Pandey A, Soccol CR (2002) Acid and enzymatic hydrolysis to recover reducing sugars from cassava bagasse: an economic study. Braz Arch Biol Technol 45:393–400
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Behera, S., Ray, R.C. Batch ethanol production from cassava (Manihot esculenta Crantz.) flour using Saccharomyces cerevisiae cells immobilized in calcium alginate. Ann Microbiol 65, 779–783 (2015). https://doi.org/10.1007/s13213-014-0918-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13213-014-0918-8